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The map label for this gene is ruvA

Identifier: 46445654

GI number: 46445654

Start: 32105

End: 32710

Strand: Direct

Name: ruvA

Synonym: pc0020

Alternate gene names: 46445654

Gene position: 32105-32710 (Clockwise)

Preceding gene: 46445653

Following gene: 46445657

Centisome position: 1.33

GC content: 36.96

Gene sequence:

>606_bases
ATGTTTGCATATATAAAAGGAGTATTAGCTTTTTTCAATCCTTCTCAAGCTATTGTTGATGTTCATGGTGTAGGATATTT
ACTTTTTATCCCTTGTCGCCTATTAGGGCAACTTCCCCAAATTGGGGAGCCAGTTCAATTTTATACCACTTATGTAGTTA
GAGAATTTTCTCACACGCTTTATGGATTTTTAAGTTACCAAGAACGCGATATTTTTGAAATTTTAATGAATGTTACAGGT
ATTGGTCCTAAGATGGCTTTAAGCTTAATCGGACATCTTTCGATGAGCGAGCTTCAAATAGCTGTTATGCGACAAGATTT
ATCTACACTCTGCCGTGTCCCAGGTGTTGGCAAGAAAACTGCGGAGCGTTTAATTGTAGAATTAAAAGATAAGTTAGCTG
CCATTGGGCATTTAGATACGTCTGATCATATAGAGCCTTTAACGCAAGATCCCAAATCCAAATCTGTTCAAGATGCCATG
TTGGCCCTTATAAATCTTGGTTATAATCAAACCACAGCCCAAAAAGCGATTAAACAAGGGATGAAAGAACTTCCAGAAGA
AATTGACTTAGCTCAATTAATTACCGTTGCTTTGAAACACGTATGA

Upstream 100 bases:

>100_bases
AGAAGATGCTGCAGATGCTTTGGCATTAGCTATTTGCCATGCACAAATGCCCATTTTAAAACAAAGTCAATATGAAACTT
GAGGAGCTAAATTAAATACT

Downstream 100 bases:

>100_bases
ATTGGAGCTTATGCCTAGGATATCAAATTGGATTTTCTACTATCCAAGAACAAATAGGGCAAATGGGAGTTTGATGTGAG
CGGGCTTGACAATGTTCTCT

Product: Holliday junction DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MFAYIKGVLAFFNPSQAIVDVHGVGYLLFIPCRLLGQLPQIGEPVQFYTTYVVREFSHTLYGFLSYQERDIFEILMNVTG
IGPKMALSLIGHLSMSELQIAVMRQDLSTLCRVPGVGKKTAERLIVELKDKLAAIGHLDTSDHIEPLTQDPKSKSVQDAM
LALINLGYNQTTAQKAIKQGMKELPEEIDLAQLITVALKHV

Sequences:

>Translated_201_residues
MFAYIKGVLAFFNPSQAIVDVHGVGYLLFIPCRLLGQLPQIGEPVQFYTTYVVREFSHTLYGFLSYQERDIFEILMNVTG
IGPKMALSLIGHLSMSELQIAVMRQDLSTLCRVPGVGKKTAERLIVELKDKLAAIGHLDTSDHIEPLTQDPKSKSVQDAM
LALINLGYNQTTAQKAIKQGMKELPEEIDLAQLITVALKHV
>Mature_201_residues
MFAYIKGVLAFFNPSQAIVDVHGVGYLLFIPCRLLGQLPQIGEPVQFYTTYVVREFSHTLYGFLSYQERDIFEILMNVTG
IGPKMALSLIGHLSMSELQIAVMRQDLSTLCRVPGVGKKTAERLIVELKDKLAAIGHLDTSDHIEPLTQDPKSKSVQDAM
LALINLGYNQTTAQKAIKQGMKELPEEIDLAQLITVALKHV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=187, Percent_Identity=35.2941176470588, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_PARUW (Q6MFA5)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007019.1
- ProteinModelPortal:   Q6MFA5
- SMR:   Q6MFA5
- STRING:   Q6MFA5
- GeneID:   2779576
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0020
- NMPDR:   fig|264201.1.peg.20
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- PhylomeDB:   Q6MFA5
- ProtClustDB:   CLSK2762040
- BioCyc:   CPRO264201:PC0020-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22343; Mature: 22343

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAYIKGVLAFFNPSQAIVDVHGVGYLLFIPCRLLGQLPQIGEPVQFYTTYVVREFSHTL
CHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YGFLSYQERDIFEILMNVTGIGPKMALSLIGHLSMSELQIAVMRQDLSTLCRVPGVGKKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AERLIVELKDKLAAIGHLDTSDHIEPLTQDPKSKSVQDAMLALINLGYNQTTAQKAIKQG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
MKELPEEIDLAQLITVALKHV
HHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MFAYIKGVLAFFNPSQAIVDVHGVGYLLFIPCRLLGQLPQIGEPVQFYTTYVVREFSHTL
CHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
YGFLSYQERDIFEILMNVTGIGPKMALSLIGHLSMSELQIAVMRQDLSTLCRVPGVGKKT
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AERLIVELKDKLAAIGHLDTSDHIEPLTQDPKSKSVQDAMLALINLGYNQTTAQKAIKQG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
MKELPEEIDLAQLITVALKHV
HHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA