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The map label for this gene is ruvC

Identifier: 46445653

GI number: 46445653

Start: 31580

End: 32086

Strand: Direct

Name: ruvC

Synonym: pc0019

Alternate gene names: 46445653

Gene position: 31580-32086 (Clockwise)

Preceding gene: 46445652

Following gene: 46445654

Centisome position: 1.31

GC content: 38.07

Gene sequence:

>507_bases
ATGAGCAATCAAGTTATTATTTTAGGATTAGACCCTGGGACAAAAATAACTGGTTTTGGAGTCATTCGCATTGAAGGCCA
TCAGTATGTTCCTGTCGATTACGGTTGTATTCGCCCTCCTTCTCATTATAAATTGTCTGAGCGTTATTTAGTCATATGTC
AAGGAGTTGAACAGTTAATCGATCAACATCAGCCTCATGCTGTCGTTGTGGAAACACAATACGTTTCTAAAAATGTTCAA
AGTGCTATGAAATTGGGAATGGCAAGAGGTGTTATCATGATTGCTGCTAAAAAAAGAGGAATTCCTATTTATGAATATGC
TCCCTCAAAAGCTAAATTAGCTGTTGTTGGGACAGGAAGAGCAAGCAAATATCAAGTTCAAGGCATGGTTCAGAGGTTAC
TTAATCTATCAATTCCTCCAACGCCAGAAGATGCTGCAGATGCTTTGGCATTAGCTATTTGCCATGCACAAATGCCCATT
TTAAAACAAAGTCAATATGAAACTTGA

Upstream 100 bases:

>100_bases
AGCCCGAGTCCGTTCAACATCCAAGCCAAGAAGACGAAGAAAAATATGATTTGTTTGGTATGAAATTTAGGCAAGATAAA
CCAAAACCAAATTCCCTTTC

Downstream 100 bases:

>100_bases
GGAGCTAAATTAAATACTATGTTTGCATATATAAAAGGAGTATTAGCTTTTTTCAATCCTTCTCAAGCTATTGTTGATGT
TCATGGTGTAGGATATTTAC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MSNQVIILGLDPGTKITGFGVIRIEGHQYVPVDYGCIRPPSHYKLSERYLVICQGVEQLIDQHQPHAVVVETQYVSKNVQ
SAMKLGMARGVIMIAAKKRGIPIYEYAPSKAKLAVVGTGRASKYQVQGMVQRLLNLSIPPTPEDAADALALAICHAQMPI
LKQSQYET

Sequences:

>Translated_168_residues
MSNQVIILGLDPGTKITGFGVIRIEGHQYVPVDYGCIRPPSHYKLSERYLVICQGVEQLIDQHQPHAVVVETQYVSKNVQ
SAMKLGMARGVIMIAAKKRGIPIYEYAPSKAKLAVVGTGRASKYQVQGMVQRLLNLSIPPTPEDAADALALAICHAQMPI
LKQSQYET
>Mature_167_residues
SNQVIILGLDPGTKITGFGVIRIEGHQYVPVDYGCIRPPSHYKLSERYLVICQGVEQLIDQHQPHAVVVETQYVSKNVQS
AMKLGMARGVIMIAAKKRGIPIYEYAPSKAKLAVVGTGRASKYQVQGMVQRLLNLSIPPTPEDAADALALAICHAQMPIL
KQSQYET

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=138, Percent_Identity=45.6521739130435, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PARUW (Q6MFA6)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007018.1
- ProteinModelPortal:   Q6MFA6
- SMR:   Q6MFA6
- STRING:   Q6MFA6
- GeneID:   2779417
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0019
- NMPDR:   fig|264201.1.peg.19
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- PhylomeDB:   Q6MFA6
- ProtClustDB:   PRK00039
- BioCyc:   CPRO264201:PC0019-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 18428; Mature: 18297

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQVIILGLDPGTKITGFGVIRIEGHQYVPVDYGCIRPPSHYKLSERYLVICQGVEQLI
CCCCEEEEECCCCCEEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
DQHQPHAVVVETQYVSKNVQSAMKLGMARGVIMIAAKKRGIPIYEYAPSKAKLAVVGTGR
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEEEEECCC
ASKYQVQGMVQRLLNLSIPPTPEDAADALALAICHAQMPILKQSQYET
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SNQVIILGLDPGTKITGFGVIRIEGHQYVPVDYGCIRPPSHYKLSERYLVICQGVEQLI
CCCEEEEECCCCCEEEEEEEEEECCCEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHH
DQHQPHAVVVETQYVSKNVQSAMKLGMARGVIMIAAKKRGIPIYEYAPSKAKLAVVGTGR
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCEEEEEECCC
ASKYQVQGMVQRLLNLSIPPTPEDAADALALAICHAQMPILKQSQYET
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA