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The map label for this gene is rfaF [H]

Identifier: 46445651

GI number: 46445651

Start: 28522

End: 29571

Strand: Reverse

Name: rfaF [H]

Synonym: pc0017

Alternate gene names: 46445651

Gene position: 29571-28522 (Counterclockwise)

Preceding gene: 46445655

Following gene: 46445650

Centisome position: 1.22

GC content: 36.67

Gene sequence:

>1050_bases
TTGTCAAAAAAAAATTTTTTATCTTTAAATCCGCAAAACATCATTATTCGTATGCCTAATTGGCTAGGAGATCTGGTGAT
GGCAACACCTATCCTTGCTGACTTAAAAAACTATTGGCCCCATGCAAAATTAACAGTCGTCTGCCAAGGATTATTGGGAG
CTGTCATTGAACATGATCCACATATCCACCATGTTATTTCCTTTGAACGTTCTAAAAGTTGGAAGCAATCACAAAAATGC
AATGAAAATTTGTTAATTTCTTTGAAGAAAGGAAATTATGATTTAGGTATTTTACTCACAAATTCATTTTCTTCTGCTTG
GAAATTTTGGCAGGGAAACGTTAAAAATAAAATCGGATATTCTACACATTGGCGTAGTTGGTTGCTTGATCATGCCATCC
CTTTTTCACCTAATTATACTCAACAACATCTTGTTGTAACCTATAAACAGTTATTAGAGCCTCTGGGTATTTCGCTCTCT
AATAACTCCCCAACGCTTTACCTCACAAAAAAAGAATTAGAGAATACTCAACAACAATTGATTTCTCATAGGGTTGAACC
TCAAGATCTAATCATTGGCATCAATCCTGGAGCTGCCTACGGGTCAGCAAAATGTTGGTTACCTGAAAGGTTTAAAGATC
TCACAGAAAAATTATTAACCAATCCTCGTACAAAAATTTTATTTTTTGGAGATAAAACAGGAACTCCTCTCGTCAATGAA
ATTTGCCAAGGGTTGCCAGACCGCGTCATTAATTTGGCTAGTAAAACTTCTATCAGAGAATTAATGGCCTATATCAAGCT
TTGCCATGTCTTTTTAACAAATGACAGTGGACCTATGCATGTAGCTGCTGCTTTAAATACTCCTCTTATTGCTTTATTTG
GATCCACAAGTGATATCGCAACAGGCCCCTATCAAGGAGGAACGGTCATCCATAAGCATGTTCCTTGTTCACCCTGTTAT
CGAAGAGAATGCCCCATTGATTTTCGGTGTATGAAACGCATTGAAGTCGAGGAAGTTTATCAGTCAATTTGTTCTTATCT
TCCTCTTTAA

Upstream 100 bases:

>100_bases
ATCATAGTCACATTTTAAAGCAGGTTTCGAAATTTATTTCTGTTTTTTGATATAAGTAAGACTTTAAAATTCGTCGCTTG
AATTAAAAAGGAAAATCGCT

Downstream 100 bases:

>100_bases
TTCATCAGCGAAGTTAAATTTTACGAATCTAACCTTTTAATTTTTTTTGCAGATTTATCGGTTAAATTGGCTAAAAAAAT
TAATTAAATTACAGTAATGG

Product: putative ADP-heptose--lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSKKNFLSLNPQNIIIRMPNWLGDLVMATPILADLKNYWPHAKLTVVCQGLLGAVIEHDPHIHHVISFERSKSWKQSQKC
NENLLISLKKGNYDLGILLTNSFSSAWKFWQGNVKNKIGYSTHWRSWLLDHAIPFSPNYTQQHLVVTYKQLLEPLGISLS
NNSPTLYLTKKELENTQQQLISHRVEPQDLIIGINPGAAYGSAKCWLPERFKDLTEKLLTNPRTKILFFGDKTGTPLVNE
ICQGLPDRVINLASKTSIRELMAYIKLCHVFLTNDSGPMHVAAALNTPLIALFGSTSDIATGPYQGGTVIHKHVPCSPCY
RRECPIDFRCMKRIEVEEVYQSICSYLPL

Sequences:

>Translated_349_residues
MSKKNFLSLNPQNIIIRMPNWLGDLVMATPILADLKNYWPHAKLTVVCQGLLGAVIEHDPHIHHVISFERSKSWKQSQKC
NENLLISLKKGNYDLGILLTNSFSSAWKFWQGNVKNKIGYSTHWRSWLLDHAIPFSPNYTQQHLVVTYKQLLEPLGISLS
NNSPTLYLTKKELENTQQQLISHRVEPQDLIIGINPGAAYGSAKCWLPERFKDLTEKLLTNPRTKILFFGDKTGTPLVNE
ICQGLPDRVINLASKTSIRELMAYIKLCHVFLTNDSGPMHVAAALNTPLIALFGSTSDIATGPYQGGTVIHKHVPCSPCY
RRECPIDFRCMKRIEVEEVYQSICSYLPL
>Mature_348_residues
SKKNFLSLNPQNIIIRMPNWLGDLVMATPILADLKNYWPHAKLTVVCQGLLGAVIEHDPHIHHVISFERSKSWKQSQKCN
ENLLISLKKGNYDLGILLTNSFSSAWKFWQGNVKNKIGYSTHWRSWLLDHAIPFSPNYTQQHLVVTYKQLLEPLGISLSN
NSPTLYLTKKELENTQQQLISHRVEPQDLIIGINPGAAYGSAKCWLPERFKDLTEKLLTNPRTKILFFGDKTGTPLVNEI
CQGLPDRVINLASKTSIRELMAYIKLCHVFLTNDSGPMHVAAALNTPLIALFGSTSDIATGPYQGGTVIHKHVPCSPCYR
RECPIDFRCMKRIEVEEVYQSICSYLPL

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=301, Percent_Identity=26.578073089701, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790062, Length=291, Percent_Identity=27.1477663230241, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 39523; Mature: 39392

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00226 IF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKNFLSLNPQNIIIRMPNWLGDLVMATPILADLKNYWPHAKLTVVCQGLLGAVIEHDP
CCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
HIHHVISFERSKSWKQSQKCNENLLISLKKGNYDLGILLTNSFSSAWKFWQGNVKNKIGY
CEEEEEEEHHHCCHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHCCCCCCCCCC
STHWRSWLLDHAIPFSPNYTQQHLVVTYKQLLEPLGISLSNNSPTLYLTKKELENTQQQL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCEEEEEHHHHHHHHHHH
ISHRVEPQDLIIGINPGAAYGSAKCWLPERFKDLTEKLLTNPRTKILFFGDKTGTPLVNE
HHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
ICQGLPDRVINLASKTSIRELMAYIKLCHVFLTNDSGPMHVAAALNTPLIALFGSTSDIA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEECCCCCCC
TGPYQGGTVIHKHVPCSPCYRRECPIDFRCMKRIEVEEVYQSICSYLPL
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKNFLSLNPQNIIIRMPNWLGDLVMATPILADLKNYWPHAKLTVVCQGLLGAVIEHDP
CCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
HIHHVISFERSKSWKQSQKCNENLLISLKKGNYDLGILLTNSFSSAWKFWQGNVKNKIGY
CEEEEEEEHHHCCHHHHHCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHCCCCCCCCCC
STHWRSWLLDHAIPFSPNYTQQHLVVTYKQLLEPLGISLSNNSPTLYLTKKELENTQQQL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEECCCCCEEEEEHHHHHHHHHHH
ISHRVEPQDLIIGINPGAAYGSAKCWLPERFKDLTEKLLTNPRTKILFFGDKTGTPLVNE
HHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHH
ICQGLPDRVINLASKTSIRELMAYIKLCHVFLTNDSGPMHVAAALNTPLIALFGSTSDIA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEECCCCCCC
TGPYQGGTVIHKHVPCSPCYRRECPIDFRCMKRIEVEEVYQSICSYLPL
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]