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The map label for this gene is hycE

Identifier: 45659240

GI number: 45659240

Start: 4199055

End: 4200455

Strand: Direct

Name: hycE

Synonym: LIC13423

Alternate gene names: 45659240

Gene position: 4199055-4200455 (Clockwise)

Preceding gene: 45659239

Following gene: 45659241

Centisome position: 98.17

GC content: 38.62

Gene sequence:

>1401_bases
ATGCGCAGCGTAACTGGTTTCTATCCTAGAAAAGGAAAAAAAGGTTACAACCGTTTCTGGCTTTCAAACGAAGGAATAGA
ACATGAAATCGTAGAATACTCCGAAAAGGAAGCTTTTGAAGATACAGCAAATCCGATTTGGATTCTCAGACATTCCTTAG
GAGTAGATCAGGGAGAAGAAGATTATTCTTCTTATGATTTTGAAAGATATATTACCGCCGATCGCAAAACAGATCTGGAA
AAATTTGTAACTAAAAAAGGTATTAAAGATTTAGTATATAAGGGATTAAAAGTACCAATTCCAGCAAGTCATTATTCCCA
CGCTGTCGGGCCGATACACGCGGGTGTAATTGAGCCGGGACATTTCAGGTTTATCGTAAAAGGTGAAGAGATTCGAAGTT
TGGATATTAGACTCGGATTTCAAAAAAGAGGTTTGATCGATTTAATCAAAGGAAAAGGTCCCAAAGATTCTATACCTTAT
GCCGAAGCGATTTCGGGAGATAGTACAATTTCCTACGCGATTGCATTTTCCAGGATTTTTGAAGAAGCCCAAAACATCGC
AGTTCCTCAAGAATTGGATTTTGCTAGATTAGTACTTTTAGAACTGGAAAGAGTCGCAACTCATATAGGAGACATGGGAG
CTATTGCGGGAGATATAGGCTATTACCCTCTACAAGGTGTATGTTCATTACAAAGAGGAATTCCTCTTGGAGTAATGGAA
GCGTTAACTGGAAATCGATTTGGAAGAGGAGCTTTGTCTCCAGGTAAAATCTTTTTTAGAAAAGGACTCGATCGAAATAA
ATTAAAAGAACTTTCAGAAAGAATTCGTTCTGTGACAGAAGAAGTCGTAGGACATTTTGAACGCGCCGCCGGAAGATCTA
CAAATCGAGAAAGACTACAATCTTGCGGAGTCATTCGACAAAAACAAGTTAGACATCTTGGATTTATAGGTATGGTGGAA
AAATGTACCGGACTAAATAGAGATTTACGTAATTTAGAAAAATCTTATGCATTTAGTTCCCCAATTTCACTAGAGTTAAA
CAGAGATCAAATGAGAGGAGACGCGTGGGCCAGATTCTATCTTCGTTATGAAGAATTAAAAAATAGCAGCAAGTGGCTTG
TAGAAGCGATTCCTAAATTGGAAGCAGCGCTCGATCTTAAGTTTGGAAAATCAAACCCAGTCAAAAAAGGAATGTATTAC
TCCGCAGTAGAAGGTTGGAGAGGACCTGTACTTGTATCCTTGGATTTGGATTCAAACGGTACGATCGAAAACACTTATAT
CCGAGAAGTTTCCGTTCCCAATTGGCACGCATTAGAATTAGCGGTACGAGGGGAATATATAGGGGACTTTCCACTAAATA
ACAAATCCTTCAATCTGAGTTACGTAGGAGTTGATTTATGA

Upstream 100 bases:

>100_bases
TACTTTACAGATCCGTATGACAAACTCTCTTTTACTTTTTATAATGGTGTTGGGTCTCGGTATATGGGGTTTTTATCATC
TACTGCAAGGAGAATTTTGA

Downstream 100 bases:

>100_bases
AAAGTATATTCGAAATATTGAATATTATTCGTCCCGCAAAGACGATGAACTATAAAAAAGTATCCCCTCTAAATCCAAAT
GCGAGAGGAATTCCTATTCC

Product: hydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRSVTGFYPRKGKKGYNRFWLSNEGIEHEIVEYSEKEAFEDTANPIWILRHSLGVDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRSLDIRLGFQKRGLIDLIKGKGPKDSIPY
AEAISGDSTISYAIAFSRIFEEAQNIAVPQELDFARLVLLELERVATHIGDMGAIAGDIGYYPLQGVCSLQRGIPLGVME
ALTGNRFGRGALSPGKIFFRKGLDRNKLKELSERIRSVTEEVVGHFERAAGRSTNRERLQSCGVIRQKQVRHLGFIGMVE
KCTGLNRDLRNLEKSYAFSSPISLELNRDQMRGDAWARFYLRYEELKNSSKWLVEAIPKLEAALDLKFGKSNPVKKGMYY
SAVEGWRGPVLVSLDLDSNGTIENTYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL

Sequences:

>Translated_466_residues
MRSVTGFYPRKGKKGYNRFWLSNEGIEHEIVEYSEKEAFEDTANPIWILRHSLGVDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRSLDIRLGFQKRGLIDLIKGKGPKDSIPY
AEAISGDSTISYAIAFSRIFEEAQNIAVPQELDFARLVLLELERVATHIGDMGAIAGDIGYYPLQGVCSLQRGIPLGVME
ALTGNRFGRGALSPGKIFFRKGLDRNKLKELSERIRSVTEEVVGHFERAAGRSTNRERLQSCGVIRQKQVRHLGFIGMVE
KCTGLNRDLRNLEKSYAFSSPISLELNRDQMRGDAWARFYLRYEELKNSSKWLVEAIPKLEAALDLKFGKSNPVKKGMYY
SAVEGWRGPVLVSLDLDSNGTIENTYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
>Mature_466_residues
MRSVTGFYPRKGKKGYNRFWLSNEGIEHEIVEYSEKEAFEDTANPIWILRHSLGVDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRSLDIRLGFQKRGLIDLIKGKGPKDSIPY
AEAISGDSTISYAIAFSRIFEEAQNIAVPQELDFARLVLLELERVATHIGDMGAIAGDIGYYPLQGVCSLQRGIPLGVME
ALTGNRFGRGALSPGKIFFRKGLDRNKLKELSERIRSVTEEVVGHFERAAGRSTNRERLQSCGVIRQKQVRHLGFIGMVE
KCTGLNRDLRNLEKSYAFSSPISLELNRDQMRGDAWARFYLRYEELKNSSKWLVEAIPKLEAALDLKFGKSNPVKKGMYY
SAVEGWRGPVLVSLDLDSNGTIENTYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Escherichia coli, GI1788832, Length=370, Percent_Identity=25.4054054054054, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789076, Length=362, Percent_Identity=23.2044198895028, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24638644, Length=284, Percent_Identity=23.2394366197183, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52611; Mature: 52611

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVTGFYPRKGKKGYNRFWLSNEGIEHEIVEYSEKEAFEDTANPIWILRHSLGVDQGEE
CCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DYSSYDFERYITADRKTDLEKFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPG
HHHCCCHHHHEECCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCC
HFRFIVKGEEIRSLDIRLGFQKRGLIDLIKGKGPKDSIPYAEAISGDSTISYAIAFSRIF
CEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
EEAQNIAVPQELDFARLVLLELERVATHIGDMGAIAGDIGYYPLQGVCSLQRGIPLGVME
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHH
ALTGNRFGRGALSPGKIFFRKGLDRNKLKELSERIRSVTEEVVGHFERAAGRSTNRERLQ
HHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SCGVIRQKQVRHLGFIGMVEKCTGLNRDLRNLEKSYAFSSPISLELNRDQMRGDAWARFY
HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCHHHHHHHH
LRYEELKNSSKWLVEAIPKLEAALDLKFGKSNPVKKGMYYSAVEGWRGPVLVSLDLDSNG
HHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCC
TIENTYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
CEECEEEEEECCCCCEEEEEEEECCEECCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRSVTGFYPRKGKKGYNRFWLSNEGIEHEIVEYSEKEAFEDTANPIWILRHSLGVDQGEE
CCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCH
DYSSYDFERYITADRKTDLEKFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPG
HHHCCCHHHHEECCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCC
HFRFIVKGEEIRSLDIRLGFQKRGLIDLIKGKGPKDSIPYAEAISGDSTISYAIAFSRIF
CEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
EEAQNIAVPQELDFARLVLLELERVATHIGDMGAIAGDIGYYPLQGVCSLQRGIPLGVME
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHH
ALTGNRFGRGALSPGKIFFRKGLDRNKLKELSERIRSVTEEVVGHFERAAGRSTNRERLQ
HHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SCGVIRQKQVRHLGFIGMVEKCTGLNRDLRNLEKSYAFSSPISLELNRDQMRGDAWARFY
HCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHHCHHHHHHHH
LRYEELKNSSKWLVEAIPKLEAALDLKFGKSNPVKKGMYYSAVEGWRGPVLVSLDLDSNG
HHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHCCCCCCEEEEEECCCCC
TIENTYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
CEECEEEEEECCCCCEEEEEEEECCEECCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]