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The map label for this gene is hyfF [H]

Identifier: 45659239

GI number: 45659239

Start: 4197846

End: 4199054

Strand: Direct

Name: hyfF [H]

Synonym: LIC13422

Alternate gene names: 45659239

Gene position: 4197846-4199054 (Clockwise)

Preceding gene: 45659238

Following gene: 45659240

Centisome position: 98.15

GC content: 38.88

Gene sequence:

>1209_bases
ATGAATCTTTTCATACTAAATGGAATCGGAGTAGTCGTTTTATTTCTGATTTTACTCGCGTATATTTTAGCACCTACTAA
GGGTCAAAAAAAAATAGCAATGTGGTCTTTTCTAATGATCCTTTGTACTGGACTTACATTCGCGGCTTGGAACTTAGAAG
GATTTGCTCTTCAATGGGTTTTAATCGAAGCGACTACTTTTACGGGAGCTTTATTAGTATCTTCTAGTAGAACTTCGAAA
TTGTTTCCGATCGCATGGAAATTTCTTTTAATCAACTCTTTTGGACTCGGGATCGCATTTTTAGGAATCATTATTCTAAC
GGCAACGTTACACGGAATCGATCATCCCGTAGAAGTTCTAGCAGGCAAATTATCCGAACATCCAGAAAACCTATGGCTTG
AGATCGGGCTTTGGTTAGCGATTTTTGGTTATTCCGCAAAATTAGGTCTTTTCCCAAATCATGTGTGGGTCGTGGATACT
TATGGAGAAAGTCCAAGTCAAATCTCCGCCTTAATCGCAGCATTCGTTCCGGTTGCGGTAAGTTATGCGCTACGTCCATT
CATCCACATGGACCACCAATTGTATCCGACAACTTTCAGTGCTTCAGACGGATTGTTGATTTTAGGAATTATAACGATGT
TACAAAGTATCGTTGCTCTCTATCAAAGAAACGATCTCAGAATGATGACTGCGAAGGTTGCCTTATTTCACAGCGGTGCA
ATTGGAATTTTTCTTTGGATGGATTTACCCGATCCAGTTTTTAACTTCGTGATGGCCGGAAACATAGTTTTAAAAGCAGT
ATTGTTCTTAACGATGGGAATCGTAAGAATGGACGCCGGTAAAAGAGAATTGAGTAAGATTTTTCTTTCTGAAAAAATCA
ACAAGAAGGCACTTTCACTTTATACTGCGTCTTTGTTCGTCGCATTTGTACTTCCAGGTTCTCCGATTTTCGTAAATGAT
CTTTTACTTTTGAAAGTCGGTTGGGCTCAAGGCCAATGGTTCGTGATCACGGTTCCATTTTTAGGATTGATCTTTTTTGG
TGTGTTACTCTACAAAACAGTTCCACTTTTTAATTTAGAGGACAGGCCTTTTGCAAAAGAGTTTGCGAGTACTTTACAGA
TCCGTATGACAAACTCTCTTTTACTTTTTATAATGGTGTTGGGTCTCGGTATATGGGGTTTTTATCATCTACTGCAAGGA
GAATTTTGA

Upstream 100 bases:

>100_bases
TTGGTTCTTTCTTAGATGCAGTGTTCATCATTGGAGCGGCTGCAACTCTTAGAATCAGTATGGAAGTTTCCTCCGAAAAC
GAAAAAGAAGAGGTGTCCGT

Downstream 100 bases:

>100_bases
ATGCGCAGCGTAACTGGTTTCTATCCTAGAAAAGGAAAAAAAGGTTACAACCGTTTCTGGCTTTCAAACGAAGGAATAGA
ACATGAAATCGTAGAATACT

Product: hydrogenase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNLFILNGIGVVVLFLILLAYILAPTKGQKKIAMWSFLMILCTGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
LFPIAWKFLLINSFGLGIAFLGIIILTATLHGIDHPVEVLAGKLSEHPENLWLEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSASDGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLPDPVFNFVMAGNIVLKAVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFVLPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFAKEFASTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF

Sequences:

>Translated_402_residues
MNLFILNGIGVVVLFLILLAYILAPTKGQKKIAMWSFLMILCTGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
LFPIAWKFLLINSFGLGIAFLGIIILTATLHGIDHPVEVLAGKLSEHPENLWLEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSASDGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLPDPVFNFVMAGNIVLKAVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFVLPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFAKEFASTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF
>Mature_402_residues
MNLFILNGIGVVVLFLILLAYILAPTKGQKKIAMWSFLMILCTGLTFAAWNLEGFALQWVLIEATTFTGALLVSSSRTSK
LFPIAWKFLLINSFGLGIAFLGIIILTATLHGIDHPVEVLAGKLSEHPENLWLEIGLWLAIFGYSAKLGLFPNHVWVVDT
YGESPSQISALIAAFVPVAVSYALRPFIHMDHQLYPTTFSASDGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
IGIFLWMDLPDPVFNFVMAGNIVLKAVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSLYTASLFVAFVLPGSPIFVND
LLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLEDRPFAKEFASTLQIRMTNSLLLFIMVLGLGIWGFYHLLQG
EF

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=290, Percent_Identity=25.1724137931034, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 44675; Mature: 44675

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFILNGIGVVVLFLILLAYILAPTKGQKKIAMWSFLMILCTGLTFAAWNLEGFALQWV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEECCCCEEEEEE
LIEATTFTGALLVSSSRTSKLFPIAWKFLLINSFGLGIAFLGIIILTATLHGIDHPVEVL
EEEEEHHCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
AGKLSEHPENLWLEIGLWLAIFGYSAKLGLFPNHVWVVDTYGESPSQISALIAAFVPVAV
HHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SYALRPFIHMDHQLYPTTFSASDGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
HHHHHHHHHCCCEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IGIFLWMDLPDPVFNFVMAGNIVLKAVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSL
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
YTASLFVAFVLPGSPIFVNDLLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLE
HHHHHHHHHHCCCCCEECCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRPFAKEFASTLQIRMTNSLLLFIMVLGLGIWGFYHLLQGEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLFILNGIGVVVLFLILLAYILAPTKGQKKIAMWSFLMILCTGLTFAAWNLEGFALQWV
CCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHEEECCCCEEEEEE
LIEATTFTGALLVSSSRTSKLFPIAWKFLLINSFGLGIAFLGIIILTATLHGIDHPVEVL
EEEEEHHCEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHH
AGKLSEHPENLWLEIGLWLAIFGYSAKLGLFPNHVWVVDTYGESPSQISALIAAFVPVAV
HHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
SYALRPFIHMDHQLYPTTFSASDGLLILGIITMLQSIVALYQRNDLRMMTAKVALFHSGA
HHHHHHHHHCCCEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IGIFLWMDLPDPVFNFVMAGNIVLKAVLFLTMGIVRMDAGKRELSKIFLSEKINKKALSL
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHH
YTASLFVAFVLPGSPIFVNDLLLLKVGWAQGQWFVITVPFLGLIFFGVLLYKTVPLFNLE
HHHHHHHHHHCCCCCEECCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCC
DRPFAKEFASTLQIRMTNSLLLFIMVLGLGIWGFYHLLQGEF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA