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The map label for this gene is cobS

Identifier: 45659176

GI number: 45659176

Start: 4115268

End: 4116065

Strand: Direct

Name: cobS

Synonym: LIC13357

Alternate gene names: 45659176

Gene position: 4115268-4116065 (Clockwise)

Preceding gene: 45659175

Following gene: 45659177

Centisome position: 96.21

GC content: 34.96

Gene sequence:

>798_bases
TTGAATAAACTCCAAGAAGAATGGAACCGTTTCTGTGCTTCTTGGATGTTCAATACGAGACTTCCAATTCTTCCATTTTA
CGTTTATTCTGAATCTACACTTTCTAGATCTTCACGTTATTTCCCTTTAATCGGTTGGATCGTTTCTGCGGGAACATCTT
ACTCTACCTATTTTCTTTCTTGGATCTTACCAATCGAAATTTCCATAATCCTTGGAATGATCTTAAGCGTTTTGATTACG
GGGGGTTTTCACGAAGACGGACTTGCAGACGTTTGCGATGCCTTCGGAGGAGGCTGGAGTAAAGAAAAAATTTTAGAGAT
TATGAAAGATAGTAGAATCGGAACATTCGGTTCTATAGGTTTGATTCTTTCGTTAGGTCTTAAATATTTATTACTCGTAA
ATCTCTTTAAGATCTCTCCTTGGATCTTTTTATTTACCTCTTGGTTTTCACACTCCGCAAGTCGATGGTTTGCTTTACTT
TTAATGATGTTAATCCCTTACGCAAGAGAAAACGATCTATCCAAATCCAAACCGATGATCAAAAAACTACCTCCTTTTGA
CTTCGCTCTCTCTACATTTTTTGGATGTTTCCCCGCGGTTTATTTTTTGTACCAATTCCAAAACCAAATTCCAAATGTAC
TTTTGGGATTTTTTCTTTCTTCTATATTTGTTTTTTATTTTAGAAATTATTTTAATAAATGGATTGAGGGTTTTACGGGA
GATTGTCTTGGGTTTATCCAACAAGGAACTGAACTTTTATTCTATTTAGGAATTACAGTTTCATGGAACTCTATTTAA

Upstream 100 bases:

>100_bases
AAGGAACCGGATGTGCAATTGCATTTCCGATTCTTATATCCGCTGTGACTTTCTTAAAAGAAATGGCCTCTTTCGAGTCC
GCAAAAGTGGATCAAAAACT

Downstream 100 bases:

>100_bases
TCCGACATACTACTCCAGAAATTCCAAAAGGAATCTGTTACGGTCAAACGGACATACCTTTAATTTCCGACTTTAATTCA
GAATTTCAATTCCTTCTTCA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNKLQEEWNRFCASWMFNTRLPILPFYVYSESTLSRSSRYFPLIGWIVSAGTSYSTYFLSWILPIEISIILGMILSVLIT
GGFHEDGLADVCDAFGGGWSKEKILEIMKDSRIGTFGSIGLILSLGLKYLLLVNLFKISPWIFLFTSWFSHSASRWFALL
LMMLIPYARENDLSKSKPMIKKLPPFDFALSTFFGCFPAVYFLYQFQNQIPNVLLGFFLSSIFVFYFRNYFNKWIEGFTG
DCLGFIQQGTELLFYLGITVSWNSI

Sequences:

>Translated_265_residues
MNKLQEEWNRFCASWMFNTRLPILPFYVYSESTLSRSSRYFPLIGWIVSAGTSYSTYFLSWILPIEISIILGMILSVLIT
GGFHEDGLADVCDAFGGGWSKEKILEIMKDSRIGTFGSIGLILSLGLKYLLLVNLFKISPWIFLFTSWFSHSASRWFALL
LMMLIPYARENDLSKSKPMIKKLPPFDFALSTFFGCFPAVYFLYQFQNQIPNVLLGFFLSSIFVFYFRNYFNKWIEGFTG
DCLGFIQQGTELLFYLGITVSWNSI
>Mature_265_residues
MNKLQEEWNRFCASWMFNTRLPILPFYVYSESTLSRSSRYFPLIGWIVSAGTSYSTYFLSWILPIEISIILGMILSVLIT
GGFHEDGLADVCDAFGGGWSKEKILEIMKDSRIGTFGSIGLILSLGLKYLLLVNLFKISPWIFLFTSWFSHSASRWFALL
LMMLIPYARENDLSKSKPMIKKLPPFDFALSTFFGCFPAVYFLYQFQNQIPNVLLGFFLSSIFVFYFRNYFNKWIEGFTG
DCLGFIQQGTELLFYLGITVSWNSI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_LEPIC (Q72M34)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_003262.1
- GeneID:   2771056
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC13357
- HOGENOM:   HBG729392
- OMA:   WICLLEL
- ProtClustDB:   CLSK575171
- BioCyc:   LINT267671:LIC_13357-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 30572; Mature: 30572

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11c56014)-; HASH(0x13c535d8)-; HASH(0x13a8f9d0)-; HASH(0x13817134)-; HASH(0x13c7cc6c)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLQEEWNRFCASWMFNTRLPILPFYVYSESTLSRSSRYFPLIGWIVSAGTSYSTYFLS
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHCCCCHHHHHHH
WILPIEISIILGMILSVLITGGFHEDGLADVCDAFGGGWSKEKILEIMKDSRIGTFGSIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
LILSLGLKYLLLVNLFKISPWIFLFTSWFSHSASRWFALLLMMLIPYARENDLSKSKPMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
KKLPPFDFALSTFFGCFPAVYFLYQFQNQIPNVLLGFFLSSIFVFYFRNYFNKWIEGFTG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DCLGFIQQGTELLFYLGITVSWNSI
HHHHHHHHHHHEEHHHEEEEECCCH
>Mature Secondary Structure
MNKLQEEWNRFCASWMFNTRLPILPFYVYSESTLSRSSRYFPLIGWIVSAGTSYSTYFLS
CCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHCCCCHHHHHHH
WILPIEISIILGMILSVLITGGFHEDGLADVCDAFGGGWSKEKILEIMKDSRIGTFGSIG
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
LILSLGLKYLLLVNLFKISPWIFLFTSWFSHSASRWFALLLMMLIPYARENDLSKSKPMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
KKLPPFDFALSTFFGCFPAVYFLYQFQNQIPNVLLGFFLSSIFVFYFRNYFNKWIEGFTG
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
DCLGFIQQGTELLFYLGITVSWNSI
HHHHHHHHHHHEEHHHEEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA