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The map label for this gene is gyrB

Identifier: 45659167

GI number: 45659167

Start: 4104748

End: 4106883

Strand: Direct

Name: gyrB

Synonym: LIC13348

Alternate gene names: 45659167

Gene position: 4104748-4106883 (Clockwise)

Preceding gene: 45659166

Following gene: 45659168

Centisome position: 95.97

GC content: 37.41

Gene sequence:

>2136_bases
ATGAGCACTGACAAAACCAAAGTAAAAGAAAAATCATCCCAAGAAAGAAATTTTAAAAAGTTATCTAACGTAGAACACGT
TCGAATGAGAACCGGTATGTGGTTAGGACAAAATTCCGCTTCCACGTTCGAACAACATTTTTTCCGCAAGAACAACGAAG
GAAAATACGAAATCGTTCACGAAGAATTAGAAGACGTTCCCGCCAAATTGAAATGTTTGGACGAGGCTTGTATGAACGCG
GTGGATGAATACCGTAAAAATCAAAAAGACAAATCAATTCCAGAAAAAGATAAGATGTCCAAATTGATCGTTCAACTTTC
CTCAGATCGCAAATGTGTAACAATTGCGGACAACGGCCGAGGAATCCCAGCCACAAACGCTGAAGGTGTTTATCTTCATT
TAATGTATGGTGAAAATTTTGACGATCACGTGAAACAAGATCACGTCGCAGGTCAAAATGGTGTTGGTATCTCTCTCGTA
AGAATGGTCTCCAACTACTTCAAAGTAAAAACTGTGAATAACGGAAGTTCTTTTAAAAAATTATTCACCGTTCATGATGA
CGTTAAAAAACAAATCCGTTCCTACAAACTTTCTAAAGAAGATACAGAGCGTGTGTTTCTCTATTTTGATGAACACGGAA
AATTTACGGATTGTAATCTTCTTACCAAAGACCAAATCGACAAACTCAGTCCTTTATTAAAAAAAACGAATATGCAAGAG
TTGATCGAAAAGGCTTCTAAAGAAGATCATGGGACTTCTGTAGAATTTGAACTCAATCCTAAATATTTTAACAACCTGGA
TATTTCGTTTAACGTAGATTTAATGAAACAATATCTACAAGACATTGCAATGACCAATCCGGGTTTAGAAGTTCAATTTG
TCTTTAAAGGTAAAAAGGAAAAATATAAGTTCAAAAAAGGACTCGATGAGATTTTTTCTCATTCTGATCTTGTTTATTAC
AAAATGGACTATGTCGCTCCGGGTTCCGCCTCTCAACTTCATCTTGAAACTTATCTTGTAATTGGTCAGAATAAAAACCT
AACTTGGGTAAATTCTATCTTTGCACCTCAAGGCGGTTCTGCGATAGAATATCTGGAAAACAGAATCTGTGACGAGGTTC
GTAAAAAAAGTCAAATTGTAGCTCTTGAGAAAAAACTCAAAACGAGTTCTACTCGTAACGACGTAAGAAATTGTTTTCAC
ATGTATGTGAACGCTAGACTTTTAAACCCACGTTTTAAATCTCAGGATAAATCCTATCTGATCAACGATCTAAACGAAGA
CATCCGTAACGCCGTAGATAAACATCTGGATAAGTTTATCAAAAAAACTGGGCTTTTAGAAGAGATCAAACTTCAGATGG
AAAAACGCACTCAACTCAAAGCTTTTGAAGACGCCCAAAAAGGTTTGAAAAAAGCGAGCAGGATGAACATTCCAAAACTC
ATGCCTCCTACTGGAAAACAAGGTGATCCTGGAAGAGTGTTGTTTGTTGCAGAAGGAGATTCTGCAATTGCGGGTCTTCG
TCCGGCAAGAAACCCTAAATTGCACGGTTTATTTCCTTTACGAGGGAAACCACTCAACTGCAAAGGCTTGAGCATAGCAA
AAGCAATCGCAAACGAAGAATTAAAAAACATAGTCGCAATCGTCGGTCTTCCTTTAGATCAAAAAGTAAAATCTTTAGAT
GAACTGAATTACGAAAAGGTAAGTATCATCACAGATGCAGATTTTGATGGTTATGCGATTCGTTCTTTGATGCTTTCTTT
TTTTTATGAGTACTGGCCAGAGCTTTTCGAACTTGGTTTTATTCATATTTCCAGCGCTCCTCTTTACGAAGTGGACGTTA
AGTTCGGAGATAAGAAAAAAGAAACCATCTTCTGTATCGATGACAAGGACTACGAAGATCTAATCAAACGAGTGGAAAAA
GGCGGCGGAGAAATCACTCGTAAAAAAAGAAACAAGGGTCTTGGTGAAACCGGTAAAGAAGCGATGAAGTTTGCGGTCGA
AGAATGTATGACCAAGATAACAATCGGAAACAAAAAAGAAGCCTCTAAAATCCAAAGCCTTTGGTTTCATAAGGATTATG
CAGAACAAAGAAGGGACGCAATTTCAGAATACGCAATGAGCGTAATCGAGGATTGA

Upstream 100 bases:

>100_bases
CTACTCTGGCTCGTTATCAAGGATTGGTCAAAGAAGATAAAAAATTGACCGAGTTTATGATCATAGAGCTGGAAGAACTT
CAAAACAAATGGGATAAAGT

Downstream 100 bases:

>100_bases
CCTCGCATTGCGTAATGGTTTCATCATTTTCGTTGCCGCTCTTTCATCGTTTTTGTTGTCGTGATTTTAAAATGTATCGG
CTGCTCGGATTCGAATGAAC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MSTDKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNA
VDEYRKNQKDKSIPEKDKMSKLIVQLSSDRKCVTIADNGRGIPATNAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLV
RMVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDTERVFLYFDEHGKFTDCNLLTKDQIDKLSPLLKKTNMQE
LIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYY
KMDYVAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFH
MYVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLDKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
MPPTGKQGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSLD
ELNYEKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETIFCIDDKDYEDLIKRVEK
GGGEITRKKRNKGLGETGKEAMKFAVEECMTKITIGNKKEASKIQSLWFHKDYAEQRRDAISEYAMSVIED

Sequences:

>Translated_711_residues
MSTDKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNA
VDEYRKNQKDKSIPEKDKMSKLIVQLSSDRKCVTIADNGRGIPATNAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLV
RMVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDTERVFLYFDEHGKFTDCNLLTKDQIDKLSPLLKKTNMQE
LIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYY
KMDYVAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFH
MYVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLDKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
MPPTGKQGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSLD
ELNYEKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETIFCIDDKDYEDLIKRVEK
GGGEITRKKRNKGLGETGKEAMKFAVEECMTKITIGNKKEASKIQSLWFHKDYAEQRRDAISEYAMSVIED
>Mature_710_residues
STDKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVHEELEDVPAKLKCLDEACMNAV
DEYRKNQKDKSIPEKDKMSKLIVQLSSDRKCVTIADNGRGIPATNAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVR
MVSNYFKVKTVNNGSSFKKLFTVHDDVKKQIRSYKLSKEDTERVFLYFDEHGKFTDCNLLTKDQIDKLSPLLKKTNMQEL
IEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKEKYKFKKGLDEIFSHSDLVYYK
MDYVAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGSAIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHM
YVNARLLNPRFKSQDKSYLINDLNEDIRNAVDKHLDKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKLM
PPTGKQGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEELKNIVAIVGLPLDQKVKSLDE
LNYEKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGFIHISSAPLYEVDVKFGDKKKETIFCIDDKDYEDLIKRVEKG
GGEITRKKRNKGLGETGKEAMKFAVEECMTKITIGNKKEASKIQSLWFHKDYAEQRRDAISEYAMSVIED

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=652, Percent_Identity=26.0736196319018, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI19913408, Length=700, Percent_Identity=26.5714285714286, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI48994957, Length=630, Percent_Identity=22.0634920634921, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789408, Length=622, Percent_Identity=21.3826366559486, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17535065, Length=714, Percent_Identity=23.249299719888, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=585, Percent_Identity=26.1538461538462, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=257, Percent_Identity=25.6809338521401, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=381, Percent_Identity=25.9842519685039, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136538, Length=623, Percent_Identity=26.0032102728732, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 81525; Mature: 81394

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVH
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCEEEHH
EELEDVPAKLKCLDEACMNAVDEYRKNQKDKSIPEKDKMSKLIVQLSSDRKCVTIADNGR
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
GIPATNAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVRMVSNYFKVKTVNNGSSFKK
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCHHHH
LFTVHDDVKKQIRSYKLSKEDTERVFLYFDEHGKFTDCNLLTKDQIDKLSPLLKKTNMQE
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCHHH
LIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKE
HHHHHCCCCCCCEEEEEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHH
KYKFKKGLDEIFSHSDLVYYKMDYVAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGS
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEEEECCCCCEEEEHEECCCCCH
AIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHMYVNARLLNPRFKSQDKSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
INDLNEDIRNAVDKHLDKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPPTGKQGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEE
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LKNIVAIVGLPLDQKVKSLDELNYEKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGF
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCE
IHISSAPLYEVDVKFGDKKKETIFCIDDKDYEDLIKRVEKGGGEITRKKRNKGLGETGKE
EEECCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
AMKFAVEECMTKITIGNKKEASKIQSLWFHKDYAEQRRDAISEYAMSVIED
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STDKTKVKEKSSQERNFKKLSNVEHVRMRTGMWLGQNSASTFEQHFFRKNNEGKYEIVH
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCEEECCCCHHHHHHHHHHCCCCCCEEEHH
EELEDVPAKLKCLDEACMNAVDEYRKNQKDKSIPEKDKMSKLIVQLSSDRKCVTIADNGR
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCC
GIPATNAEGVYLHLMYGENFDDHVKQDHVAGQNGVGISLVRMVSNYFKVKTVNNGSSFKK
CCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEEECCCCHHHH
LFTVHDDVKKQIRSYKLSKEDTERVFLYFDEHGKFTDCNLLTKDQIDKLSPLLKKTNMQE
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCHHH
LIEKASKEDHGTSVEFELNPKYFNNLDISFNVDLMKQYLQDIAMTNPGLEVQFVFKGKKE
HHHHHCCCCCCCEEEEEECCHHHCCCCEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHH
KYKFKKGLDEIFSHSDLVYYKMDYVAPGSASQLHLETYLVIGQNKNLTWVNSIFAPQGGS
HHHHHHHHHHHHCCCCEEEEEEEEECCCCCCEEEEEEEEEEECCCCCEEEEHEECCCCCH
AIEYLENRICDEVRKKSQIVALEKKLKTSSTRNDVRNCFHMYVNARLLNPRFKSQDKSYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHH
INDLNEDIRNAVDKHLDKFIKKTGLLEEIKLQMEKRTQLKAFEDAQKGLKKASRMNIPKL
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MPPTGKQGDPGRVLFVAEGDSAIAGLRPARNPKLHGLFPLRGKPLNCKGLSIAKAIANEE
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
LKNIVAIVGLPLDQKVKSLDELNYEKVSIITDADFDGYAIRSLMLSFFYEYWPELFELGF
HHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCE
IHISSAPLYEVDVKFGDKKKETIFCIDDKDYEDLIKRVEKGGGEITRKKRNKGLGETGKE
EEECCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHH
AMKFAVEECMTKITIGNKKEASKIQSLWFHKDYAEQRRDAISEYAMSVIED
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]