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The map label for this gene is phoB [H]

Identifier: 45659090

GI number: 45659090

Start: 4007977

End: 4008678

Strand: Direct

Name: phoB [H]

Synonym: LIC13269

Alternate gene names: 45659090

Gene position: 4007977-4008678 (Clockwise)

Preceding gene: 45659089

Following gene: 45659091

Centisome position: 93.71

GC content: 38.32

Gene sequence:

>702_bases
ATGAAAAATCAATCAGGGAACCCAGGGCAAAAAGTTCTCGTCGTAGACGATGAGGAAGATATAGCCGAGCTGATCCGATT
TCATTTAGAAGAAAACGGTTACCAAGTAGATACATGCCAAAATGGATTAGAAGTACTTCCAAAATTGGAAAAAAATACTC
CTGACTTAATCATCTTAGATCTTATGCTTCCCGGAATCGGAGGTATGGATCTTTGCAAGAAAATCAAAGAAAAGTATTCT
ATGCCGATCATTATGGTTACCGCAAAATCTGGAGAAACCGAAGCAGTTCTCGGTTTGGAATTAGGGGCGGACGATTATGT
TCGTAAACCATTTAGCACAAGGGAATTAATCGCCAGGGTACGCTCCGTTTTAAGAAGAATCAAAGATGAAGAAGAAGATG
AATTCGAAGGAAATATCACGATTGGAAATATCTTTCTCAATCTGAAAGCGCATAAAGCCTTCATAAATAACTCTGAAGTC
GATTTAACTTTAATAGAGTATAAAATACTAAATCTTTTTATGACCAATCCAGGAGTCGCCTTTACTAGAGATAAACTATT
GGATCGAGTCTGGGGAAAAGATATTTACGTTACCGATAGAGCAGTAGACGTAAATATCAAAAGACTACGCGATAAACTCG
GAGACGAAAAGGAAAGACTAGAAACGATACGCGGAATCGGCTATAGATTCAATGAGGCGTAG

Upstream 100 bases:

>100_bases
GATGATAAGACCTACCCGGTGCTCTTGCAGGCTCCGATTTTTTAAAAAGAAAATCGGTTTGTAAAAAACTACGATAAACC
GGTAAAAAAGTACAGGAATC

Downstream 100 bases:

>100_bases
TTTATTTTCAAAACTTCTTCTTAGTAATTGGCTACTTCTACTTGTACTTCTTGTGGTCGCCGGTATTGCTCTTTTCGTAG
AGAAGTTCGTTCATTCTGAT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLIILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRIKDEEEDEFEGNITIGNIFLNLKAHKAFINNSEV
DLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA

Sequences:

>Translated_233_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLIILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRIKDEEEDEFEGNITIGNIFLNLKAHKAFINNSEV
DLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
>Mature_233_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLIILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRIKDEEEDEFEGNITIGNIFLNLKAHKAFINNSEV
DLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=39.4736842105263, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1788394, Length=223, Percent_Identity=37.6681614349776, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=39.5555555555556, Blast_Score=158, Evalue=2e-40,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=37.4449339207048, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=36.8888888888889, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=35.2941176470588, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1790552, Length=219, Percent_Identity=32.8767123287671, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=34.8017621145374, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=32.8828828828829, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787229, Length=223, Percent_Identity=32.7354260089686, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=33.1838565022422, Blast_Score=117, Evalue=4e-28,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=31.5789473684211, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=30.0448430493274, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=29.7413793103448, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790299, Length=122, Percent_Identity=36.0655737704918, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=29.9065420560748, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26573; Mature: 26573

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLIILD
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCEEEEE
LMLPGIGGMDLCKKIKEKYSMPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARV
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
RSVLRRIKDEEEDEFEGNITIGNIFLNLKAHKAFINNSEVDLTLIEYKILNLFMTNPGVA
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEHEEEECCCCEEEEEHHEEEEHHEECCCCCC
FTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
HHHHHHHHHHCCCCEEEECCEEECHHHHHHHHHCCHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLIILD
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCCCEEEEE
LMLPGIGGMDLCKKIKEKYSMPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARV
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
RSVLRRIKDEEEDEFEGNITIGNIFLNLKAHKAFINNSEVDLTLIEYKILNLFMTNPGVA
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEHEEEECCCCEEEEEHHEEEEHHEECCCCCC
FTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
HHHHHHHHHHCCCCEEEECCEEECHHHHHHHHHCCHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]