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The map label for this gene is nsdHL

Identifier: 45658955

GI number: 45658955

Start: 3837388

End: 3838353

Strand: Reverse

Name: nsdHL

Synonym: LIC13133

Alternate gene names: 45658955

Gene position: 3838353-3837388 (Counterclockwise)

Preceding gene: 45658960

Following gene: 45658954

Centisome position: 89.74

GC content: 40.06

Gene sequence:

>966_bases
ATGAAAATTTTTATAACAGGAGCGTCCGGTTTTGTAGGAGAAGCAACAACCCGGACCTTATCAAAAAAACATACTATTTT
AGCGATGTCTCGATCTGAAAAAACGGATTTAGTCATATCCAACGCAGGAGGAAAACCGGTTCGATGTGAACTTAATTCGG
TAAATCCAAGTTCCCTACAAGGGATCGACGTGGTCATTCATAGTGCGGCTTATGTGGAGCAATGGGGACCTTTCCAAGAT
TTTTGGAAAATAAATGTGGAGGGGACTGCTCAGCTTTTAGAGGCGTCTCGCAAAGCGGGTGTTAAACGATTTATTTTTAT
CGGAACCGAAGCGGCACTTTTTTACGGACAACCGATGATCGATATAGATGAATCTTATCCTTATCCGGAAAATTCTCCTT
TTCCTTATTCTAAAACCAAAGCCGAAGCAGAAAAATTAGTACTAAAAGCCAATTCTTCCGAGATGCAGACGCTTTCAATT
CGTCCTCGTTTAATTTGGGGGCCGGGAGATAAAACGGTTCTTCCAATTCTTTTGAAGATGATTGCTGAAGGAAAATTTTC
TTGGATTGACAGCGGAAAAGCTCTAACTAGTACAACTCATATTTATAACTTAGTACATGCCATTGAACTGGCTCTTACCA
AAGGTCAAGGCGGTAGTGCTTATTTTGTAACGGATGATGAAATATTCAATTTTCGTAATTTTTTAGAGTCTCTACTAGCG
ACTCAGAAGGTAACGGCGCCAAATCGTTCGATTCCAGGATGGTTTGCTCGTTTTTTAGCAAGAATTGTCGAAGCGGTTTG
GAAATTATTTGGTATTAAAAATGAACCTCCTTTGACCCGTTTTAGCGCAAGTATCATGTCCAGAGATTGTACGATTAAAA
TAGATAATGCTAAAAAAGATTTAGGTTATTCTCCCTTATTGACGGTTCGCCAAGGGCTCGAAGGGATGCCGGTTCTAAAA
TCGTGA

Upstream 100 bases:

>100_bases
CTTTGTATATATTAATTAATATTAGAAAATCAATATTTATTAATATAATAAATAAACTTTAGAAAATAATAAATTATTTA
TATTTAAGGAGAGCAAAGAA

Downstream 100 bases:

>100_bases
CATTCAAAAGTTAGAATATAGAATCTGCTTTATATATTTCTGAGTTCTAAAGGATAATAATTATTCGGAACTACTTAATA
GAATATCCAACCCAAATGAT

Product: NAD(P)H steroid dehydrogenase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKIFITGASGFVGEATTRTLSKKHTILAMSRSEKTDLVISNAGGKPVRCELNSVNPSSLQGIDVVIHSAAYVEQWGPFQD
FWKINVEGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPENSPFPYSKTKAEAEKLVLKANSSEMQTLSI
RPRLIWGPGDKTVLPILLKMIAEGKFSWIDSGKALTSTTHIYNLVHAIELALTKGQGGSAYFVTDDEIFNFRNFLESLLA
TQKVTAPNRSIPGWFARFLARIVEAVWKLFGIKNEPPLTRFSASIMSRDCTIKIDNAKKDLGYSPLLTVRQGLEGMPVLK
S

Sequences:

>Translated_321_residues
MKIFITGASGFVGEATTRTLSKKHTILAMSRSEKTDLVISNAGGKPVRCELNSVNPSSLQGIDVVIHSAAYVEQWGPFQD
FWKINVEGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPENSPFPYSKTKAEAEKLVLKANSSEMQTLSI
RPRLIWGPGDKTVLPILLKMIAEGKFSWIDSGKALTSTTHIYNLVHAIELALTKGQGGSAYFVTDDEIFNFRNFLESLLA
TQKVTAPNRSIPGWFARFLARIVEAVWKLFGIKNEPPLTRFSASIMSRDCTIKIDNAKKDLGYSPLLTVRQGLEGMPVLK
S
>Mature_321_residues
MKIFITGASGFVGEATTRTLSKKHTILAMSRSEKTDLVISNAGGKPVRCELNSVNPSSLQGIDVVIHSAAYVEQWGPFQD
FWKINVEGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMIDIDESYPYPENSPFPYSKTKAEAEKLVLKANSSEMQTLSI
RPRLIWGPGDKTVLPILLKMIAEGKFSWIDSGKALTSTTHIYNLVHAIELALTKGQGGSAYFVTDDEIFNFRNFLESLLA
TQKVTAPNRSIPGWFARFLARIVEAVWKLFGIKNEPPLTRFSASIMSRDCTIKIDNAKKDLGYSPLLTVRQGLEGMPVLK
S

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=326, Percent_Identity=28.5276073619632, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI8393516, Length=326, Percent_Identity=28.5276073619632, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI116268111, Length=331, Percent_Identity=29.3051359516616, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI310132178, Length=340, Percent_Identity=28.8235294117647, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310113012, Length=340, Percent_Identity=28.8235294117647, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI239745448, Length=336, Percent_Identity=28.8690476190476, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI19923621, Length=345, Percent_Identity=23.1884057971014, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4504507, Length=353, Percent_Identity=24.929178470255, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260763931, Length=285, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4504509, Length=285, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081792, Length=326, Percent_Identity=28.8343558282209, Blast_Score=128, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI193211272, Length=332, Percent_Identity=27.4096385542169, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17570557, Length=322, Percent_Identity=27.9503105590062, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17509709, Length=275, Percent_Identity=27.6363636363636, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321437, Length=281, Percent_Identity=30.2491103202847, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 35529; Mature: 35529

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFITGASGFVGEATTRTLSKKHTILAMSRSEKTDLVISNAGGKPVRCELNSVNPSSLQ
CEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCC
GIDVVIHSAAYVEQWGPFQDFWKINVEGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMI
CCHHEEHHHHHHHHCCCCCHHEEEECCHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCE
DIDESYPYPENSPFPYSKTKAEAEKLVLKANSSEMQTLSIRPRLIWGPGDKTVLPILLKM
ECCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCEEEEEECCEEEECCCCCCHHHHHHHH
IAEGKFSWIDSGKALTSTTHIYNLVHAIELALTKGQGGSAYFVTDDEIFNFRNFLESLLA
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHH
TQKVTAPNRSIPGWFARFLARIVEAVWKLFGIKNEPPLTRFSASIMSRDCTIKIDNAKKD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
LGYSPLLTVRQGLEGMPVLKS
CCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKIFITGASGFVGEATTRTLSKKHTILAMSRSEKTDLVISNAGGKPVRCELNSVNPSSLQ
CEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCCCC
GIDVVIHSAAYVEQWGPFQDFWKINVEGTAQLLEASRKAGVKRFIFIGTEAALFYGQPMI
CCHHEEHHHHHHHHCCCCCHHEEEECCHHHHHHHHHHHCCCEEEEEEECCEEEEECCCCE
DIDESYPYPENSPFPYSKTKAEAEKLVLKANSSEMQTLSIRPRLIWGPGDKTVLPILLKM
ECCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCEEEEEECCEEEECCCCCCHHHHHHHH
IAEGKFSWIDSGKALTSTTHIYNLVHAIELALTKGQGGSAYFVTDDEIFNFRNFLESLLA
HHCCCEEEECCCCCHHHHHHHHHHHHHHHHEEECCCCCEEEEEECHHHHHHHHHHHHHHH
TQKVTAPNRSIPGWFARFLARIVEAVWKLFGIKNEPPLTRFSASIMSRDCTIKIDNAKKD
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCCCCC
LGYSPLLTVRQGLEGMPVLKS
CCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]