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The map label for this gene is liaS [H]

Identifier: 45658913

GI number: 45658913

Start: 3773916

End: 3774977

Strand: Direct

Name: liaS [H]

Synonym: LIC13087

Alternate gene names: 45658913

Gene position: 3773916-3774977 (Clockwise)

Preceding gene: 45658912

Following gene: 45658914

Centisome position: 88.23

GC content: 37.01

Gene sequence:

>1062_bases
ATGCAGGATTTGAAACGTAAGAATCGAAAACGTCTTACCACTACAACGATATTGAAACCAGGTCTTCCGCCTAAATTTTT
TCTCAATCTTCTTAAAGAGATTTCTCCCATCGTAGCAATTGACAACACTCAAACGATTCTGTTTGCAAACCAATCCTTTC
AAAAAGAATTTGCTGGAAGCTCTCGGAGTTTGATCGGTAAAAACCTATTTCGAATTCTGAACCTAAATCCGGTAGACCAC
GAAGAAATGGAATCAAACATCAATTTATCCAAATGGGGAAAAATACAAAATCAAGAATTTAGAAAACAAAAAATCTATTA
CGGTTACTCCGTATTTCGTTTTGGAGATCACATAGGAATTATTCTCAAAAACATTACAGAAAACAAAAGATTGGAAAGAA
AAATTGCAAGTCTTCATACCCAACTCCTACAATCCCAAGAAGAAGAAAGAATTAGACTTTCCGGAGAATTGCACGATGGA
GTTGGTCAAACCATCCTCGCGGCAAAACTCAACTTCCAAGCATACAATCGAAATCCTAAAGTCTATGGGGCACAGTTCGA
TACGGGACTTCTTTTGATAGATAAGGCAAGCCAAGAACTAAGAGATATTTATACAAATTTACATCCTTCCACACTCAGAG
AAATCGGCTTAGAAGCCGCAATTCGATCCTTAAGTTCGGATCTATTTCCGCCTATGGACATAGAAGCAGATTTAGACTTA
AATCTCAAATTGAATCTAGAGCAGACAACGGCCAATCAGGTTTTTAGAATCGTTCAGGAAATCTTTCAGAACGTGATTAA
ACATTCCCAAGCGAGTAAAATTTCTCTCAAGATTCAAGCCAAAGGAAGACAGTTGTTTATAGACGCTAAAGATAACGGCA
TCGGTTTTCAGGAAAGAAAGGTCAGAGCGAGGTCCTCCGGTTTTGGACTTGAAAATATCCGTAGAAGAGTAGAAGATTTA
AATGGTAAAATTAAGATTGATTCTTCTGCTGGGAAAGGAACTAAGTTTATCATCCGAATTCCTTTGGAAAAAAACAAAAA
CACTTACGCTAAAAAAACATGA

Upstream 100 bases:

>100_bases
TTTCCTGCATCGAACTGAATTCATTTTCCCAGGAATTTTTTCCTGGGAATTCTTTTTTTCTTTTCTTTCTTTGAGGTAAA
ACAAAAGTGTTTAACCTCTT

Downstream 100 bases:

>100_bases
AACCTACCGATACAAAAATATTTCTAGTCGATGATCACGCAATTCTTAGAGAAGGATTAAAAATGATTCTCTCCGGCCAA
CCCGGTTTCGAAATTTGTGG

Product: histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQDLKRKNRKRLTTTTILKPGLPPKFFLNLLKEISPIVAIDNTQTILFANQSFQKEFAGSSRSLIGKNLFRILNLNPVDH
EEMESNINLSKWGKIQNQEFRKQKIYYGYSVFRFGDHIGIILKNITENKRLERKIASLHTQLLQSQEEERIRLSGELHDG
VGQTILAAKLNFQAYNRNPKVYGAQFDTGLLLIDKASQELRDIYTNLHPSTLREIGLEAAIRSLSSDLFPPMDIEADLDL
NLKLNLEQTTANQVFRIVQEIFQNVIKHSQASKISLKIQAKGRQLFIDAKDNGIGFQERKVRARSSGFGLENIRRRVEDL
NGKIKIDSSAGKGTKFIIRIPLEKNKNTYAKKT

Sequences:

>Translated_353_residues
MQDLKRKNRKRLTTTTILKPGLPPKFFLNLLKEISPIVAIDNTQTILFANQSFQKEFAGSSRSLIGKNLFRILNLNPVDH
EEMESNINLSKWGKIQNQEFRKQKIYYGYSVFRFGDHIGIILKNITENKRLERKIASLHTQLLQSQEEERIRLSGELHDG
VGQTILAAKLNFQAYNRNPKVYGAQFDTGLLLIDKASQELRDIYTNLHPSTLREIGLEAAIRSLSSDLFPPMDIEADLDL
NLKLNLEQTTANQVFRIVQEIFQNVIKHSQASKISLKIQAKGRQLFIDAKDNGIGFQERKVRARSSGFGLENIRRRVEDL
NGKIKIDSSAGKGTKFIIRIPLEKNKNTYAKKT
>Mature_353_residues
MQDLKRKNRKRLTTTTILKPGLPPKFFLNLLKEISPIVAIDNTQTILFANQSFQKEFAGSSRSLIGKNLFRILNLNPVDH
EEMESNINLSKWGKIQNQEFRKQKIYYGYSVFRFGDHIGIILKNITENKRLERKIASLHTQLLQSQEEERIRLSGELHDG
VGQTILAAKLNFQAYNRNPKVYGAQFDTGLLLIDKASQELRDIYTNLHPSTLREIGLEAAIRSLSSDLFPPMDIEADLDL
NLKLNLEQTTANQVFRIVQEIFQNVIKHSQASKISLKIQAKGRQLFIDAKDNGIGFQERKVRARSSGFGLENIRRRVEDL
NGKIKIDSSAGKGTKFIIRIPLEKNKNTYAKKT

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=227, Percent_Identity=28.1938325991189, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787474, Length=206, Percent_Identity=24.7572815533981, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40377; Mature: 40377

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLKRKNRKRLTTTTILKPGLPPKFFLNLLKEISPIVAIDNTQTILFANQSFQKEFAGS
CCCHHHHHHHHCCHHEEECCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCHHHHHHCCC
SRSLIGKNLFRILNLNPVDHEEMESNINLSKWGKIQNQEFRKQKIYYGYSVFRFGDHIGI
CHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCCHHHHHHHHHCCEEEEECCCCHHH
ILKNITENKRLERKIASLHTQLLQSQEEERIRLSGELHDGVGQTILAAKLNFQAYNRNPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCHHCCCCCEEEEEEEEEEEECCCCC
VYGAQFDTGLLLIDKASQELRDIYTNLHPSTLREIGLEAAIRSLSSDLFPPMDIEADLDL
EEEEECCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
NLKLNLEQTTANQVFRIVQEIFQNVIKHSQASKISLKIQAKGRQLFIDAKDNGIGFQERK
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHH
VRARSSGFGLENIRRRVEDLNGKIKIDSSAGKGTKFIIRIPLEKNKNTYAKKT
HHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MQDLKRKNRKRLTTTTILKPGLPPKFFLNLLKEISPIVAIDNTQTILFANQSFQKEFAGS
CCCHHHHHHHHCCHHEEECCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCHHHHHHCCC
SRSLIGKNLFRILNLNPVDHEEMESNINLSKWGKIQNQEFRKQKIYYGYSVFRFGDHIGI
CHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHCCCCCCHHHHHHHHHCCEEEEECCCCHHH
ILKNITENKRLERKIASLHTQLLQSQEEERIRLSGELHDGVGQTILAAKLNFQAYNRNPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCHHCCCCCEEEEEEEEEEEECCCCC
VYGAQFDTGLLLIDKASQELRDIYTNLHPSTLREIGLEAAIRSLSSDLFPPMDIEADLDL
EEEEECCCCEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
NLKLNLEQTTANQVFRIVQEIFQNVIKHSQASKISLKIQAKGRQLFIDAKDNGIGFQERK
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCHHHHH
VRARSSGFGLENIRRRVEDLNGKIKIDSSAGKGTKFIIRIPLEKNKNTYAKKT
HHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]