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The map label for this gene is murD

Identifier: 45658619

GI number: 45658619

Start: 3393136

End: 3394527

Strand: Direct

Name: murD

Synonym: LIC12788

Alternate gene names: 45658619

Gene position: 3393136-3394527 (Clockwise)

Preceding gene: 45658599

Following gene: 45658620

Centisome position: 79.33

GC content: 34.34

Gene sequence:

>1392_bases
ATGAAATTTCCAGAGTCCTTAAAAGGCCTAAAAATTCTCGTTTTAGGGGGAGGAATCTCCGGAAACTCGGCTCTTAATTT
TTTAATTTCCGAAAAAGCCCAGCCGATTCTTTGCGATCGAAATCAACCAGAAAGAACCGTTGTTCCCTTTTTTCCAGATA
ATATTCCCCCTCAGTCTCTTCCTGAAGTTTCATTGGTTATTAAATCTCCTGGAATTTTACCAACTCATCCAATTCTTTCT
TATGCTGCGGATAAAAAAATTCCGGTTGTATCTGAGATTGATTTAGGAAGATATTTTTTTAAAGGAAAAATTATAGGCAT
TACCGGAACCGACGGTAAATCTACTACAACTTCTTTAATTGCGCACCTTTTGAAAGAAAGTTTTCCCGATCTAAAAGAAG
GTGGAAATTTAGGAATTCCTTTTACCTCTTTTTGTAAAGAATCCATTTCCCTGGCCGTTCTAGAACTCTCTAGTTATCAA
CTTGAAGATTCATCTCCTCTTCATCTGGATGTGTCCGTTTTTCTGAATCTTGCTTCTGATCATTTAGAAAGACACAAAAC
GATGGAAAATTATTTCCAAGCTAAACTTAAAATTGCGGATCTATCTAATTCAAATCATACTTTGATAGTTTCTGAAAAAA
TCAAAGAAAGAATTTTAAATTCTATTTCTTATCAGTGTAAACTTCTTAGTTTCGGAAAAACTTCCGATTCAAATGCATTT
TTAGACGAAAACTCTTTGAAAATCAAAACTTCAAAGTTTGTATACGACATCTCTAAGTTTTATCTTCCAGGAACTCATAA
TAGAGAAAATCTCGCTGCGTCCATTCTCGCTGCAGAAGAAATTGGTGGAAAACCAGAATCAATCCAAACTAGGATTCCTC
TTTTTAGAGGTTTACCTCATCGTTTTCAAATCGCTGGAGAAAAATTAGGAATTTCGTTTATTAACGATTCTAAATCAACG
AACTTACATAGTATGCTCGCGGGAATGGCTACTTGGAAAAATATAGATCAGACTTGTTTAATTTTAGGCGGTAGACCTAA
GCAAGAAGACTTAAAACCACTTTACAATTTTCTAATCAAAGGAATTGGCTGTGTAGTTTTATTTGGAGAGGCCAGGGCCA
CTTGGGAAAGTGGAATTAAAAATATAATCGGAGAAAAGCTCTACTGCGTTGAAAATCTAAATGATACTTTTGAAATATTC
AAAAAAGGAAATATATTTCCCGTCCCCGGTTTAAACAAAGATATAATAATTCGTCTTTCCGATAGCATTTCAATTTCCTC
TTTTGTTTTTTCTCCGGCTTGTGCAAGTTTTGATCAATATAAGAATTTCGAAGAAAGGGGAAATCATTTTCTTTCCCTAG
TGAATGATTTTCTAGACCAAATTGATTCTTAA

Upstream 100 bases:

>100_bases
TACTTTATGATCGAGCAAGGGAATAATCCTTATAGTTTTTTGCTACTGACAACTATCGGGGGCGAAAATAACCTGTCAAT
CACTGTTCAACGATTTAAAA

Downstream 100 bases:

>100_bases
AATTTTATCTATTGAGTTATAAATATTAACGTTTTCTATCTTTAGTTGGGGGCTTACGGGGAATTTTTACATTTTCGAAA
TTTTACAGTAAAATCTAAAT

Product: UDP-n-acetylmuramoylalanine--d-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKFPESLKGLKILVLGGGISGNSALNFLISEKAQPILCDRNQPERTVVPFFPDNIPPQSLPEVSLVIKSPGILPTHPILS
YAADKKIPVVSEIDLGRYFFKGKIIGITGTDGKSTTTSLIAHLLKESFPDLKEGGNLGIPFTSFCKESISLAVLELSSYQ
LEDSSPLHLDVSVFLNLASDHLERHKTMENYFQAKLKIADLSNSNHTLIVSEKIKERILNSISYQCKLLSFGKTSDSNAF
LDENSLKIKTSKFVYDISKFYLPGTHNRENLAASILAAEEIGGKPESIQTRIPLFRGLPHRFQIAGEKLGISFINDSKST
NLHSMLAGMATWKNIDQTCLILGGRPKQEDLKPLYNFLIKGIGCVVLFGEARATWESGIKNIIGEKLYCVENLNDTFEIF
KKGNIFPVPGLNKDIIIRLSDSISISSFVFSPACASFDQYKNFEERGNHFLSLVNDFLDQIDS

Sequences:

>Translated_463_residues
MKFPESLKGLKILVLGGGISGNSALNFLISEKAQPILCDRNQPERTVVPFFPDNIPPQSLPEVSLVIKSPGILPTHPILS
YAADKKIPVVSEIDLGRYFFKGKIIGITGTDGKSTTTSLIAHLLKESFPDLKEGGNLGIPFTSFCKESISLAVLELSSYQ
LEDSSPLHLDVSVFLNLASDHLERHKTMENYFQAKLKIADLSNSNHTLIVSEKIKERILNSISYQCKLLSFGKTSDSNAF
LDENSLKIKTSKFVYDISKFYLPGTHNRENLAASILAAEEIGGKPESIQTRIPLFRGLPHRFQIAGEKLGISFINDSKST
NLHSMLAGMATWKNIDQTCLILGGRPKQEDLKPLYNFLIKGIGCVVLFGEARATWESGIKNIIGEKLYCVENLNDTFEIF
KKGNIFPVPGLNKDIIIRLSDSISISSFVFSPACASFDQYKNFEERGNHFLSLVNDFLDQIDS
>Mature_463_residues
MKFPESLKGLKILVLGGGISGNSALNFLISEKAQPILCDRNQPERTVVPFFPDNIPPQSLPEVSLVIKSPGILPTHPILS
YAADKKIPVVSEIDLGRYFFKGKIIGITGTDGKSTTTSLIAHLLKESFPDLKEGGNLGIPFTSFCKESISLAVLELSSYQ
LEDSSPLHLDVSVFLNLASDHLERHKTMENYFQAKLKIADLSNSNHTLIVSEKIKERILNSISYQCKLLSFGKTSDSNAF
LDENSLKIKTSKFVYDISKFYLPGTHNRENLAASILAAEEIGGKPESIQTRIPLFRGLPHRFQIAGEKLGISFINDSKST
NLHSMLAGMATWKNIDQTCLILGGRPKQEDLKPLYNFLIKGIGCVVLFGEARATWESGIKNIIGEKLYCVENLNDTFEIF
KKGNIFPVPGLNKDIIIRLSDSISISSFVFSPACASFDQYKNFEERGNHFLSLVNDFLDQIDS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=463, Percent_Identity=30.2375809935205, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_LEPIC (Q72NP4)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002705.1
- ProteinModelPortal:   Q72NP4
- GeneID:   2772440
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12788
- NMPDR:   fig|267671.1.peg.2705
- HOGENOM:   HBG529092
- OMA:   LWVDDSK
- ProtClustDB:   CLSK573665
- BioCyc:   LINT267671:LIC_12788-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 51299; Mature: 51299

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPESLKGLKILVLGGGISGNSALNFLISEKAQPILCDRNQPERTVVPFFPDNIPPQSL
CCCCCCCCCCEEEEEECCCCCCHHEEEEECCCCCCEEECCCCCCCEEECCCCCCCCCCCC
PEVSLVIKSPGILPTHPILSYAADKKIPVVSEIDLGRYFFKGKIIGITGTDGKSTTTSLI
CCEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEEEECCCCCHHHHHHH
AHLLKESFPDLKEGGNLGIPFTSFCKESISLAVLELSSYQLEDSSPLHLDVSVFLNLASD
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHEEEEEEECCEECCCCCCCEEEHHHHHHHHHH
HLERHKTMENYFQAKLKIADLSNSNHTLIVSEKIKERILNSISYQCKLLSFGKTSDSNAF
HHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCCCCCCCE
LDENSLKIKTSKFVYDISKFYLPGTHNRENLAASILAAEEIGGKPESIQTRIPLFRGLPH
ECCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHHCCCC
RFQIAGEKLGISFINDSKSTNLHSMLAGMATWKNIDQTCLILGGRPKQEDLKPLYNFLIK
CEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
GIGCVVLFGEARATWESGIKNIIGEKLYCVENLNDTFEIFKKGNIFPVPGLNKDIIIRLS
CCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEEECCCCCCCEEEEEE
DSISISSFVFSPACASFDQYKNFEERGNHFLSLVNDFLDQIDS
CCCEEHHHCCCHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFPESLKGLKILVLGGGISGNSALNFLISEKAQPILCDRNQPERTVVPFFPDNIPPQSL
CCCCCCCCCCEEEEEECCCCCCHHEEEEECCCCCCEEECCCCCCCEEECCCCCCCCCCCC
PEVSLVIKSPGILPTHPILSYAADKKIPVVSEIDLGRYFFKGKIIGITGTDGKSTTTSLI
CCEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCEEEEEEEEEEEECCCCCHHHHHHH
AHLLKESFPDLKEGGNLGIPFTSFCKESISLAVLELSSYQLEDSSPLHLDVSVFLNLASD
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHEEEEEEECCEECCCCCCCEEEHHHHHHHHHH
HLERHKTMENYFQAKLKIADLSNSNHTLIVSEKIKERILNSISYQCKLLSFGKTSDSNAF
HHHHHHHHHHHHHHEEEEEECCCCCCEEEEHHHHHHHHHHHHCCEEEEEECCCCCCCCCE
LDENSLKIKTSKFVYDISKFYLPGTHNRENLAASILAAEEIGGKPESIQTRIPLFRGLPH
ECCCCEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCHHHHCCCC
RFQIAGEKLGISFINDSKSTNLHSMLAGMATWKNIDQTCLILGGRPKQEDLKPLYNFLIK
CEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH
GIGCVVLFGEARATWESGIKNIIGEKLYCVENLNDTFEIFKKGNIFPVPGLNKDIIIRLS
CCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCEEECCCCCCCEEEEEE
DSISISSFVFSPACASFDQYKNFEERGNHFLSLVNDFLDQIDS
CCCEEHHHCCCHHHHCHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA