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The map label for this gene is rlmN

Identifier: 45658616

GI number: 45658616

Start: 3389804

End: 3390865

Strand: Reverse

Name: rlmN

Synonym: LIC12785

Alternate gene names: 45658616

Gene position: 3390865-3389804 (Counterclockwise)

Preceding gene: 45658617

Following gene: 45658615

Centisome position: 79.28

GC content: 38.32

Gene sequence:

>1062_bases
ATGATTTCAGAAAACCTTGGAGAAAACCAAACCGAAAAAATACCGTTAAAAGGAAGAACTCTAAAAGAACTTTCTGAAAT
CATGATTACTCTGGGAGAAAAACCTTTCCGAGCAAAACAAATCTATCATGGTTTGTATGTAAATCGTTACGAAACCTGGG
ACCAATTTACTACATTCTCTAAAATCTTCAAAGAAAAACTCGAAGAACTTTGTTCTCTTACTCATCTTCAAGTTGTAAAA
CAACTTAAATCCGTGGACGGAACACAAAAATTCACGTTCACGTCCGAATCAGGAAATGGAAAAGAATTCGAAGCGGTTTG
GATTCCATCTGGAGACGGTGGAAGAAAAACGATCTGCATCTCTTCCCAGATAGGTTGTACTCTCAATTGTAAATTCTGTG
CAACTGCAAAATTAGAATTTCAAGGAAACCTAAAGGCACATGAGATCGTAGATCAGATATTACAGGTGGAAAAAATCGTA
GGAGACAAAGCGACTAACGTTGTATTTATGGGAATGGGGGAACCACTTCATAATTATTTCAACGTGATTCGTGCTGCCTC
CATCTTTCACGATCCGGATGCGCTCAATTTGGGAGCCAAAAGAATTACGATCTCAACTTCCGGAGTAGTAAACGGGATCA
GGCGTTTTATAGAAAATAAAGAACCTTATAATTTTGCAATTTCACTCAATCATCCGGATCCAAAGGGCAGATTACAAATC
ATGGATATCGAAGAGAAATTTTCTCTTCCCGAACTTTTACAAGCGGCCAAAGATTTTACGAGAGAGTTAAAAAGAAGAAT
CACTTTCGAGTACGTAATGATTCCTGGAGTGAATATGGGGTTTGAAAACGCGAATAAACTCGTAAAAATTGCTAAGTCAC
TTGATTGTAAAATTAACGTGATTCCTCTCAATACCGAATTTTTCGGTTGGAGAAGACCTACTCGAGAAGAAATCGCAGAG
TTTATTGCTCTTCTTGAACCCGCCGGAGTTCCTATACTTAATAGAAGGTCGCCTGGCAAAGATATTTTTGGAGCTTGTGG
AATGCTCGCTTCAAAAAGTTGA

Upstream 100 bases:

>100_bases
GTAAATTTGTAACGAAAGATTATTTTTTGTGATGAACTTATAGTTTGAAAAGAGTATCCGTTCCAGGGAGCTGAAAAAAA
ATCTACCAAGAAGGTCGTTT

Downstream 100 bases:

>100_bases
CCTACACTTTCGTTTCCGAAAAATGGGGCCATGAATCCAAAAAGAACTATTATTCTTTTTTTATATCTTTTACCTTTCGT
TTCCTGCCAGGAATATGTTC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MISENLGENQTEKIPLKGRTLKELSEIMITLGEKPFRAKQIYHGLYVNRYETWDQFTTFSKIFKEKLEELCSLTHLQVVK
QLKSVDGTQKFTFTSESGNGKEFEAVWIPSGDGGRKTICISSQIGCTLNCKFCATAKLEFQGNLKAHEIVDQILQVEKIV
GDKATNVVFMGMGEPLHNYFNVIRAASIFHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPKGRLQI
MDIEEKFSLPELLQAAKDFTRELKRRITFEYVMIPGVNMGFENANKLVKIAKSLDCKINVIPLNTEFFGWRRPTREEIAE
FIALLEPAGVPILNRRSPGKDIFGACGMLASKS

Sequences:

>Translated_353_residues
MISENLGENQTEKIPLKGRTLKELSEIMITLGEKPFRAKQIYHGLYVNRYETWDQFTTFSKIFKEKLEELCSLTHLQVVK
QLKSVDGTQKFTFTSESGNGKEFEAVWIPSGDGGRKTICISSQIGCTLNCKFCATAKLEFQGNLKAHEIVDQILQVEKIV
GDKATNVVFMGMGEPLHNYFNVIRAASIFHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPKGRLQI
MDIEEKFSLPELLQAAKDFTRELKRRITFEYVMIPGVNMGFENANKLVKIAKSLDCKINVIPLNTEFFGWRRPTREEIAE
FIALLEPAGVPILNRRSPGKDIFGACGMLASKS
>Mature_353_residues
MISENLGENQTEKIPLKGRTLKELSEIMITLGEKPFRAKQIYHGLYVNRYETWDQFTTFSKIFKEKLEELCSLTHLQVVK
QLKSVDGTQKFTFTSESGNGKEFEAVWIPSGDGGRKTICISSQIGCTLNCKFCATAKLEFQGNLKAHEIVDQILQVEKIV
GDKATNVVFMGMGEPLHNYFNVIRAASIFHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPKGRLQI
MDIEEKFSLPELLQAAKDFTRELKRRITFEYVMIPGVNMGFENANKLVKIAKSLDCKINVIPLNTEFFGWRRPTREEIAE
FIALLEPAGVPILNRRSPGKDIFGACGMLASKS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=350, Percent_Identity=37.1428571428571, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_LEPIC (Q72NP7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002702.1
- ProteinModelPortal:   Q72NP7
- GeneID:   2770467
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12785
- NMPDR:   fig|267671.1.peg.2702
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14465
- BioCyc:   LINT267671:LIC_12785-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39802; Mature: 39802

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISENLGENQTEKIPLKGRTLKELSEIMITLGEKPFRAKQIYHGLYVNRYETWDQFTTFS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECHHHHHHHHHHH
KIFKEKLEELCSLTHLQVVKQLKSVDGTQKFTFTSESGNGKEFEAVWIPSGDGGRKTICI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEE
SSQIGCTLNCKFCATAKLEFQGNLKAHEIVDQILQVEKIVGDKATNVVFMGMGEPLHNYF
ECCCCEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
NVIRAASIFHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPKGRLQI
HHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
MDIEEKFSLPELLQAAKDFTRELKRRITFEYVMIPGVNMGFENANKLVKIAKSLDCKINV
EECHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
IPLNTEFFGWRRPTREEIAEFIALLEPAGVPILNRRSPGKDIFGACGMLASKS
EEECCHHCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MISENLGENQTEKIPLKGRTLKELSEIMITLGEKPFRAKQIYHGLYVNRYETWDQFTTFS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECHHHHHHHHHHH
KIFKEKLEELCSLTHLQVVKQLKSVDGTQKFTFTSESGNGKEFEAVWIPSGDGGRKTICI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEE
SSQIGCTLNCKFCATAKLEFQGNLKAHEIVDQILQVEKIVGDKATNVVFMGMGEPLHNYF
ECCCCEEEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
NVIRAASIFHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPKGRLQI
HHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEE
MDIEEKFSLPELLQAAKDFTRELKRRITFEYVMIPGVNMGFENANKLVKIAKSLDCKINV
EECHHHCCHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEE
IPLNTEFFGWRRPTREEIAEFIALLEPAGVPILNRRSPGKDIFGACGMLASKS
EEECCHHCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA