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The map label for this gene is nuoN

Identifier: 45658586

GI number: 45658586

Start: 3348766

End: 3350304

Strand: Direct

Name: nuoN

Synonym: LIC12752

Alternate gene names: 45658586

Gene position: 3348766-3350304 (Clockwise)

Preceding gene: 45658585

Following gene: 45658589

Centisome position: 78.29

GC content: 37.88

Gene sequence:

>1539_bases
ATGAATCTATTTGATTTATCTATTTTACCATTTTTAAATCTTGCACAGGTCGTTAATGAAAGTAGGGTTCCGAACGTGCC
AGACTTACTTTCCGTTTTACCTGCGCTCGTCTTGGCGACAGGCGGAGTATTTTTGATTTGTTTATCCGTATTATTTAAAA
CGAAAGAATTTATAATCGTAAGATACGTTTCGGGACTGATTTTACTTTTGGCATTTGCGGCCGTATTTTATACTTCGTTT
CGATTTCCAGGAAACGGATTTCACTTTAGTGGGCAAATTGAAAATTCCGTATTATCTTTTTGGCTAAATTTAATTTATAT
TTCTATGGCAATTGGAACCGCTGCAATAGTTCCGAGAATATTAAAAAATCATAAAATAGAATTCCCTGAATTTTATCCAC
TACTTCTATTTGCAACTTGTGGAATGACTCTAATGACTACGGGACAAGATTTTATACTAGTGTTCGTGGCTTTAGAACTA
ATGAGCATCTGTTTATATATATTGATAGGGATGGCAAGAAGTGATCTGTTTTCTTTAGAAGCCACATTGAAATATTTTCT
ATTAGGAAGTTTTTCTTCCGGATTTATGTTGATGGGTATTGCTTTTTTATTCGGAGGAAGCGGGTCTACAAATATTACCG
AGGCTTTAAAACCACTGACCATCGCCGGTTATCAAGGAAACTTTGCCAAGATAGGTTTAGTATTGTTCATCACAGGAGTT
GCGTTTAAGATAGCATTGTTTCCATATCACGCTTGGACCCCGGATGCATATGAAGGCGCTTTAACACCTGTTACAGGTTA
TATGTCCACTGCAGCAAAAGCTGCGTCGATCGGGCTACTTTTAATACTTTATACAAAATTACCACTTCCACTTGAGAATA
GTACCTGGGCGTGGTTGCCCGGAATTCTTGCATTATGTTCTATGATCTACGGAAACCTACTCGCATTAAAACAAGAAAAT
TTAAAACGAATGCTTGCGTATTCTTCGATTGCACACGCAGGTTATGTAGTAGCAGGAATTTCTGCAGGGATCAAAGAGGA
AGTATTATTTTATCTAATCGTATATTCATTTATGAGTTTGGGAGCGTTTGCGATCCTCGCATATTTGGAAGAAGGAACCA
GACAAGTGACTTTCTTTTCCGTTCAATCCTTATCTGGAGTAAAACCTTTAACTGCAATAGCAATTAACATTTTCTTCATG
TCTTTAGCGGGAGTTCCTCCCTTTGGAGGTTTTTGGGCAAAGTTATTCTTATTTCAAAAACTCGCAGAATCGGAAACACT
CATGAATCGGATATTACTCATCGGTGGGGTAACTAATTCAGCATTAGCACTTTATTATTATCTAAGAATTGGAATCGCAA
CTTTTATGAGTAGCGACGAAGGAGAAATATCCAGGAACCACGCAGCGCCTTACAGCGTAGGAGTAACCGGGGTCGTATTA
TTTTGTCTTTTAATGGTCTCGGTAGGATGGTTTTTGTTAGTTCCAGGCAACTTACTAGCGTTAGGTGAATTGCGGTTATC
AAGTTCCATCTTTCGTTGA

Upstream 100 bases:

>100_bases
GAGTAGAACCGTCTAAATATGCGGAACGTCTGCAAAGATATCCAAGCAAATATTCCTTACCGGAACCAATTCGAAAAAAT
TCCGATGAGGAGACACAAAA

Downstream 100 bases:

>100_bases
ATTTAAGAACAAACACCACAATTTACAACCACTGGAATATAATTCGATCCAAAAGTCCAATTTTAAAACCAATCGATAAC
TTGATTTTACCGTTTTTAAA

Product: NADH dehydrogenase I subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MNLFDLSILPFLNLAQVVNESRVPNVPDLLSVLPALVLATGGVFLICLSVLFKTKEFIIVRYVSGLILLLAFAAVFYTSF
RFPGNGFHFSGQIENSVLSFWLNLIYISMAIGTAAIVPRILKNHKIEFPEFYPLLLFATCGMTLMTTGQDFILVFVALEL
MSICLYILIGMARSDLFSLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITEALKPLTIAGYQGNFAKIGLVLFITGV
AFKIALFPYHAWTPDAYEGALTPVTGYMSTAAKAASIGLLLILYTKLPLPLENSTWAWLPGILALCSMIYGNLLALKQEN
LKRMLAYSSIAHAGYVVAGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEEGTRQVTFFSVQSLSGVKPLTAIAINIFFM
SLAGVPPFGGFWAKLFLFQKLAESETLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPYSVGVTGVVL
FCLLMVSVGWFLLVPGNLLALGELRLSSSIFR

Sequences:

>Translated_512_residues
MNLFDLSILPFLNLAQVVNESRVPNVPDLLSVLPALVLATGGVFLICLSVLFKTKEFIIVRYVSGLILLLAFAAVFYTSF
RFPGNGFHFSGQIENSVLSFWLNLIYISMAIGTAAIVPRILKNHKIEFPEFYPLLLFATCGMTLMTTGQDFILVFVALEL
MSICLYILIGMARSDLFSLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITEALKPLTIAGYQGNFAKIGLVLFITGV
AFKIALFPYHAWTPDAYEGALTPVTGYMSTAAKAASIGLLLILYTKLPLPLENSTWAWLPGILALCSMIYGNLLALKQEN
LKRMLAYSSIAHAGYVVAGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEEGTRQVTFFSVQSLSGVKPLTAIAINIFFM
SLAGVPPFGGFWAKLFLFQKLAESETLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPYSVGVTGVVL
FCLLMVSVGWFLLVPGNLLALGELRLSSSIFR
>Mature_512_residues
MNLFDLSILPFLNLAQVVNESRVPNVPDLLSVLPALVLATGGVFLICLSVLFKTKEFIIVRYVSGLILLLAFAAVFYTSF
RFPGNGFHFSGQIENSVLSFWLNLIYISMAIGTAAIVPRILKNHKIEFPEFYPLLLFATCGMTLMTTGQDFILVFVALEL
MSICLYILIGMARSDLFSLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITEALKPLTIAGYQGNFAKIGLVLFITGV
AFKIALFPYHAWTPDAYEGALTPVTGYMSTAAKAASIGLLLILYTKLPLPLENSTWAWLPGILALCSMIYGNLLALKQEN
LKRMLAYSSIAHAGYVVAGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEEGTRQVTFFSVQSLSGVKPLTAIAINIFFM
SLAGVPPFGGFWAKLFLFQKLAESETLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPYSVGVTGVVL
FCLLMVSVGWFLLVPGNLLALGELRLSSSIFR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=378, Percent_Identity=32.5396825396825, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1788831, Length=306, Percent_Identity=26.1437908496732, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788827, Length=294, Percent_Identity=26.8707482993197, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788829, Length=314, Percent_Identity=27.0700636942675, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788614, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788613, Length=280, Percent_Identity=27.1428571428571, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55843; Mature: 55843

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFDLSILPFLNLAQVVNESRVPNVPDLLSVLPALVLATGGVFLICLSVLFKTKEFIIV
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RYVSGLILLLAFAAVFYTSFRFPGNGFHFSGQIENSVLSFWLNLIYISMAIGTAAIVPRI
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKNHKIEFPEFYPLLLFATCGMTLMTTGQDFILVFVALELMSICLYILIGMARSDLFSLE
HHCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITEALKPLTIAGYQGNFAKIGLVLFITGV
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHH
AFKIALFPYHAWTPDAYEGALTPVTGYMSTAAKAASIGLLLILYTKLPLPLENSTWAWLP
HHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GILALCSMIYGNLLALKQENLKRMLAYSSIAHAGYVVAGISAGIKEEVLFYLIVYSFMSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHH
GAFAILAYLEEGTRQVTFFSVQSLSGVKPLTAIAINIFFMSLAGVPPFGGFWAKLFLFQK
HHHHHHHHHHHCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LAESETLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPYSVGVTGVVL
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
FCLLMVSVGWFLLVPGNLLALGELRLSSSIFR
HHHHHHHCCHHEEECCCEEEEHHHHHHHHCCC
>Mature Secondary Structure
MNLFDLSILPFLNLAQVVNESRVPNVPDLLSVLPALVLATGGVFLICLSVLFKTKEFIIV
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RYVSGLILLLAFAAVFYTSFRFPGNGFHFSGQIENSVLSFWLNLIYISMAIGTAAIVPRI
HHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKNHKIEFPEFYPLLLFATCGMTLMTTGQDFILVFVALELMSICLYILIGMARSDLFSLE
HHCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITEALKPLTIAGYQGNFAKIGLVLFITGV
HHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCEEECCCCCHHHHHHHHHHHHH
AFKIALFPYHAWTPDAYEGALTPVTGYMSTAAKAASIGLLLILYTKLPLPLENSTWAWLP
HHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GILALCSMIYGNLLALKQENLKRMLAYSSIAHAGYVVAGISAGIKEEVLFYLIVYSFMSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHH
GAFAILAYLEEGTRQVTFFSVQSLSGVKPLTAIAINIFFMSLAGVPPFGGFWAKLFLFQK
HHHHHHHHHHHCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LAESETLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPYSVGVTGVVL
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
FCLLMVSVGWFLLVPGNLLALGELRLSSSIFR
HHHHHHHCCHHEEECCCEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]