Click here to switch to the map view.

The map label for this gene is nuoF

Identifier: 45658580

GI number: 45658580

Start: 3341725

End: 3343056

Strand: Direct

Name: nuoF

Synonym: LIC12746

Alternate gene names: 45658580

Gene position: 3341725-3343056 (Clockwise)

Preceding gene: 45658579

Following gene: 45658581

Centisome position: 78.13

GC content: 44.59

Gene sequence:

>1332_bases
ATGGCAGAAATGAAACTTCTTACGAAATACATCGACAATCCTCAGTCCACCAAACTGGAGTTTTACGAATCCGTACACGG
ATACGATGGAATGAAAAAAGCGCTTCAGATGAAACCAGACGACATCATCGCAGAAGTCAAAAAATCCGGACTGAGAGGAA
GGGGTGGAGCCGGTTTTCCGACCGGTCTTAAATGGTCGTTTATTCCCAAAGACATTCCAAAACCGAAATATATCATTTGT
AACGCGGACGAAGGAGAACCGGGAACATTCAAAGATCGTAAACTGATTGAGAATCTTCCACATCAGATTATAGAAGGGAT
GATCATAGGAGCGCGCGCGATCGGTTCCAACAAAGGATTTTTTTATATTCGAGGAGAATTCCAAAAAGGCGCAAAAGCCA
TACAGGCGGCGATCGACGAAGCCTACTCCAAAGGATATTTAGGAAAGAATATTTTAGGAAGCGGTTTTGATTTTGATCTG
ATTCTTTACGAAGGAGCGGGCGCTTATATCTGTGGAGAAGAAACCGCGCTGATCAATTCACTCGAAGGAAGAAGGGGCCA
TCCTAGATTAAAACCCCCCTTCCCCGCCGTGTCGGGACTTTATCGTTCTCCGACCGTAGTCAATAACGTAGAAACTTTTT
CGGCGATTCCTCATATTTTAGATAAGGGAGCGGATTGGTATTCTAAAATAGGAACGGAAAAATCTCCGGGCACGAGATTA
TTCAGCGTATCCGGTCACGTAAAAAAACCGGGCGTTTACGAAATCGAATTAGGAACTCCTTTATTAGAATTAGTGAATGA
TCTTTGTGGTGGAATCATAGACGATAAACCTCTCAAGGCGATCATTCCGGGAGGATCCTCCGTTCCGATTCTCACCGCGG
AAGAATGTAAAACCGCAAATATGGATTTTGAATCCATGGCCGCTCATAAAACCATGCTCGGCTCTGGAGCGGTAATCGTT
CTTGCGGAAGGAACTGACATTGTAGAAACCACGTATCGATTCGCCCGTTTTTACGCACACGAATCCTGCGGTCAGTGCAC
TCCTTGTAGAGAAGGAACACATTGGGTCAGGGACATTCTCTATAAAATTAGAGAAGGAGAAGGAACAACCGAAGACATAG
ATCTGATTTTTTCTTTATCCAGAAATATGGAAGGAGGAACCACGATCTGTCCACTTTCAGACGCTTGTGTAGGAGCGGTC
CGACCAGCGTTACAAAAGTTCCGCTCCGAGTTTGACGCAAAATTGAAAGTAAAACCGATCGTACCCGATCATCCTCGAAG
AAGAAAAGAAGATAAGATCGAGTCGACTGCAGGAGAAAAGACAAGTCCATGA

Upstream 100 bases:

>100_bases
CGCTCCTATGGTGCAAATTAACGACGACTTTTACGAACATCTGACTCCCGAAAAAGTAGATCAGATTTTAGACGATCTGA
ACTGACAAAAGGAAATCCTT

Downstream 100 bases:

>100_bases
ACTGGAACGAAATTCTATTTTGGCTTTTAAAAAGCGGGCTTTTCTTTTTTATACTCATCACTGCATGCGCATACTATACT
CTCGCAGAAAGAAAGGTCGC

Product: NADH dehydrogenase subunit I F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MAEMKLLTKYIDNPQSTKLEFYESVHGYDGMKKALQMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDIPKPKYIIC
NADEGEPGTFKDRKLIENLPHQIIEGMIIGARAIGSNKGFFYIRGEFQKGAKAIQAAIDEAYSKGYLGKNILGSGFDFDL
ILYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
FSVSGHVKKPGVYEIELGTPLLELVNDLCGGIIDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIV
LAEGTDIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTEDIDLIFSLSRNMEGGTTICPLSDACVGAV
RPALQKFRSEFDAKLKVKPIVPDHPRRRKEDKIESTAGEKTSP

Sequences:

>Translated_443_residues
MAEMKLLTKYIDNPQSTKLEFYESVHGYDGMKKALQMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDIPKPKYIIC
NADEGEPGTFKDRKLIENLPHQIIEGMIIGARAIGSNKGFFYIRGEFQKGAKAIQAAIDEAYSKGYLGKNILGSGFDFDL
ILYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
FSVSGHVKKPGVYEIELGTPLLELVNDLCGGIIDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIV
LAEGTDIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTEDIDLIFSLSRNMEGGTTICPLSDACVGAV
RPALQKFRSEFDAKLKVKPIVPDHPRRRKEDKIESTAGEKTSP
>Mature_442_residues
AEMKLLTKYIDNPQSTKLEFYESVHGYDGMKKALQMKPDDIIAEVKKSGLRGRGGAGFPTGLKWSFIPKDIPKPKYIICN
ADEGEPGTFKDRKLIENLPHQIIEGMIIGARAIGSNKGFFYIRGEFQKGAKAIQAAIDEAYSKGYLGKNILGSGFDFDLI
LYEGAGAYICGEETALINSLEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRLF
SVSGHVKKPGVYEIELGTPLLELVNDLCGGIIDDKPLKAIIPGGSSVPILTAEECKTANMDFESMAAHKTMLGSGAVIVL
AEGTDIVETTYRFARFYAHESCGQCTPCREGTHWVRDILYKIREGEGTTEDIDLIFSLSRNMEGGTTICPLSDACVGAVR
PALQKFRSEFDAKLKVKPIVPDHPRRRKEDKIESTAGEKTSP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=388, Percent_Identity=48.7113402061856, Blast_Score=390, Evalue=1e-109,
Organism=Homo sapiens, GI260656005, Length=388, Percent_Identity=48.7113402061856, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1788620, Length=421, Percent_Identity=45.6057007125891, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17531727, Length=378, Percent_Identity=50.2645502645503, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45550452, Length=401, Percent_Identity=48.1296758104738, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19920794, Length=381, Percent_Identity=47.7690288713911, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24653819, Length=386, Percent_Identity=36.5284974093264, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24653817, Length=357, Percent_Identity=34.1736694677871, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48530; Mature: 48399

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMKLLTKYIDNPQSTKLEFYESVHGYDGMKKALQMKPDDIIAEVKKSGLRGRGGAGFP
CCHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
TGLKWSFIPKDIPKPKYIICNADEGEPGTFKDRKLIENLPHQIIEGMIIGARAIGSNKGF
CCCEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEHEEECCCCCE
FYIRGEFQKGAKAIQAAIDEAYSKGYLGKNILGSGFDFDLILYEGAGAYICGEETALINS
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
CCCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEE
FSVSGHVKKPGVYEIELGTPLLELVNDLCGGIIDDKPLKAIIPGGSSVPILTAEECKTAN
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHCCCCC
MDFESMAAHKTMLGSGAVIVLAEGTDIVETTYRFARFYAHESCGQCTPCREGTHWVRDIL
CCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YKIREGEGTTEDIDLIFSLSRNMEGGTTICPLSDACVGAVRPALQKFRSEFDAKLKVKPI
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
VPDHPRRRKEDKIESTAGEKTSP
CCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEMKLLTKYIDNPQSTKLEFYESVHGYDGMKKALQMKPDDIIAEVKKSGLRGRGGAGFP
CHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCC
TGLKWSFIPKDIPKPKYIICNADEGEPGTFKDRKLIENLPHQIIEGMIIGARAIGSNKGF
CCCEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEEEEHEEECCCCCE
FYIRGEFQKGAKAIQAAIDEAYSKGYLGKNILGSGFDFDLILYEGAGAYICGEETALINS
EEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCHHHHHHH
LEGRRGHPRLKPPFPAVSGLYRSPTVVNNVETFSAIPHILDKGADWYSKIGTEKSPGTRL
CCCCCCCCCCCCCCHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEE
FSVSGHVKKPGVYEIELGTPLLELVNDLCGGIIDDKPLKAIIPGGSSVPILTAEECKTAN
EEECCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEHHHCCCCC
MDFESMAAHKTMLGSGAVIVLAEGTDIVETTYRFARFYAHESCGQCTPCREGTHWVRDIL
CCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YKIREGEGTTEDIDLIFSLSRNMEGGTTICPLSDACVGAVRPALQKFRSEFDAKLKVKPI
HHHHCCCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
VPDHPRRRKEDKIESTAGEKTSP
CCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]