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The map label for this gene is nusA

Identifier: 45658541

GI number: 45658541

Start: 3292762

End: 3294144

Strand: Reverse

Name: nusA

Synonym: LIC12706

Alternate gene names: 45658541

Gene position: 3294144-3292762 (Counterclockwise)

Preceding gene: 45658542

Following gene: 45658540

Centisome position: 77.02

GC content: 41.65

Gene sequence:

>1383_bases
ATGGCAGTTAAGAAGATACAATCGGAAGGAAATCTGTTGGAAGCGATCCAACAATTTTGTGCGGATAAATCCCTGGATAG
GGAAGCAGTTATGGGAGTCATTCGAGATTCTTTGATAACCGCATATAAAAAAAAATCCGGACTGGAGGAATCTTCAGAAT
TGGAAAAACCTTCTCCAGTTACCGTAGAGTTTGCTTCTGGAAAAGATAGCGTAGTCATCGCAATTGCAAAAAAGGTAGTT
TCTGGAAATCCTTCTTCCGCATTGGAAATCAGTTTAGAGGATGCTAAAGCAATTGATGCATCAGCGGAAATTGGATCCGT
AATTTCTTTTCGAGAAAAACCAGTAGAACTTTCCAGAATCATTTCTAGCCAAGCGAAACAAATGGTGTTTCAAAGACTCA
AAGATATGGAGAAAGAACTTCTCTTTAACGAGTATAAGGCGAAGGAAGGAGAGCTGACTCACGGATATTTTCAGAGATGG
AAAAAAGATGCCATGAGCATCGATCTCGGAAAGGTGGAAGGGATTATGCCCCGCCGAGAACAAAATCCGGGTGAAAAGTA
TCATAGCGGAGATAGACTCAAAGCAATTATTCAAAGGGTAGAATTACGTCCTAGAGAACCAATTCCGGTCATTACACTTT
CTAGGGCATCTGCGGATTTTGTTCGCAAATTGTTCGAAATGGAAATTCCAGAAATCTACGATGGTTTAGTCGAAATTATC
AATGTAGCTAGACAACCTTCTATTCGTACAAAAGTTGTAGTACGCGCTACCCGCGGAGACATAGACCCAGTAGGGGCTTG
TGTAGGTATGAAAGGGGTTCGGATTCAATCAATCGTAAGAGAACTTGGAAACGAAAGGATTGATATTGTAGAATCCTCCG
ATGACGCTTCCGAATTTATAGCAAATGCAATTTCTCCTGCAAAACCGGTGGAAGTAAAAGTGGACGGTTCTGGTAGAGAA
GCAATGGTAGTCGTGCCTGATGATCAACTTTCTCTTGCGATCGGAATCAATGGATCTAATGTAAAACTCGCTTCTCAGTT
GGCTGGTTACAAAATTGATATTAAAACCGTGACACAATATAACGCCGAACTTGCTTCACCAGAAGCAAGAGAAAGGCTGG
AAAGACTTTTTTACTCTCCTGTGGAAGAAACCAAAGTTTCGATGGAACAAGAAGAGGAGGAAGAACTTACGCCTTTGGAA
GATCTTCCTGGTCTCTCTGCTCGTATCGTAGGAATTTTGAAGAGCGAGGGAATTAAAGATTTGGAAACTCTAATTGAGTA
TAGCCAAGACGATCTGGCAAAATTACAAGGAATCGGACATACTACAGCCGGACAGATTCTCAAATTGCTCCGAGAGTCCG
TAGAATGGGTGGAGGATAACTGA

Upstream 100 bases:

>100_bases
TTGGAAAAATTCCAAAAGGGAAAAAAATCTGTCGTAAAAAAGCAGACGACACTGAACCTCAAGGATATACTGAAAGGGAA
TCTTTACGTAAATATTTGAT

Downstream 100 bases:

>100_bases
AAAAGCCGGAACGGTGGATTCAGATCTGGGGTTTTTAGGACTTCGGAAAGCCAGGGAATCATTTATGGAAGATAAAAATA
AGACGATCAAGGAAACGCTT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAVKKIQSEGNLLEAIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEESSELEKPSPVTVEFASGKDSVVIAIAKKVV
SGNPSSALEISLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLFNEYKAKEGELTHGYFQRW
KKDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVEII
NVARQPSIRTKVVVRATRGDIDPVGACVGMKGVRIQSIVRELGNERIDIVESSDDASEFIANAISPAKPVEVKVDGSGRE
AMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVTQYNAELASPEARERLERLFYSPVEETKVSMEQEEEEELTPLE
DLPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN

Sequences:

>Translated_460_residues
MAVKKIQSEGNLLEAIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEESSELEKPSPVTVEFASGKDSVVIAIAKKVV
SGNPSSALEISLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLFNEYKAKEGELTHGYFQRW
KKDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVEII
NVARQPSIRTKVVVRATRGDIDPVGACVGMKGVRIQSIVRELGNERIDIVESSDDASEFIANAISPAKPVEVKVDGSGRE
AMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVTQYNAELASPEARERLERLFYSPVEETKVSMEQEEEEELTPLE
DLPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
>Mature_459_residues
AVKKIQSEGNLLEAIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEESSELEKPSPVTVEFASGKDSVVIAIAKKVVS
GNPSSALEISLEDAKAIDASAEIGSVISFREKPVELSRIISSQAKQMVFQRLKDMEKELLFNEYKAKEGELTHGYFQRWK
KDAMSIDLGKVEGIMPRREQNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVEIIN
VARQPSIRTKVVVRATRGDIDPVGACVGMKGVRIQSIVRELGNERIDIVESSDDASEFIANAISPAKPVEVKVDGSGREA
MVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVTQYNAELASPEARERLERLFYSPVEETKVSMEQEEEEELTPLED
LPGLSARIVGILKSEGIKDLETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=501, Percent_Identity=29.940119760479, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 50894; Mature: 50763

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVKKIQSEGNLLEAIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEESSELEKPSPV
CCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCE
TVEFASGKDSVVIAIAKKVVSGNPSSALEISLEDAKAIDASAEIGSVISFREKPVELSRI
EEEECCCCCCEEEEEHHHHHCCCCCCEEEEEECHHHHHCCHHHHHHHHHHHCCCHHHHHH
ISSQAKQMVFQRLKDMEKELLFNEYKAKEGELTHGYFQRWKKDAMSIDLGKVEGIMPRRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEHHHHHCCCCCCC
QNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVEII
CCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
NVARQPSIRTKVVVRATRGDIDPVGACVGMKGVRIQSIVRELGNERIDIVESSDDASEFI
HHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHH
ANAISPAKPVEVKVDGSGREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVTQY
HHHCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCEEHHHHHCCCEEEEEEHHHH
NAELASPEARERLERLFYSPVEETKVSMEQEEEEELTPLEDLPGLSARIVGILKSEGIKD
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCHHH
LETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
HHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVKKIQSEGNLLEAIQQFCADKSLDREAVMGVIRDSLITAYKKKSGLEESSELEKPSPV
CCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCE
TVEFASGKDSVVIAIAKKVVSGNPSSALEISLEDAKAIDASAEIGSVISFREKPVELSRI
EEEECCCCCCEEEEEHHHHHCCCCCCEEEEEECHHHHHCCHHHHHHHHHHHCCCHHHHHH
ISSQAKQMVFQRLKDMEKELLFNEYKAKEGELTHGYFQRWKKDAMSIDLGKVEGIMPRRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEEHHHHHCCCCCCC
QNPGEKYHSGDRLKAIIQRVELRPREPIPVITLSRASADFVRKLFEMEIPEIYDGLVEII
CCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHH
NVARQPSIRTKVVVRATRGDIDPVGACVGMKGVRIQSIVRELGNERIDIVESSDDASEFI
HHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHH
ANAISPAKPVEVKVDGSGREAMVVVPDDQLSLAIGINGSNVKLASQLAGYKIDIKTVTQY
HHHCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCCCCEEHHHHHCCCEEEEEEHHHH
NAELASPEARERLERLFYSPVEETKVSMEQEEEEELTPLEDLPGLSARIVGILKSEGIKD
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCHHH
LETLIEYSQDDLAKLQGIGHTTAGQILKLLRESVEWVEDN
HHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]