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The map label for this gene is purA

Identifier: 45658406

GI number: 45658406

Start: 3110859

End: 3112136

Strand: Direct

Name: purA

Synonym: LIC12565

Alternate gene names: 45658406

Gene position: 3110859-3112136 (Clockwise)

Preceding gene: 45658405

Following gene: 45658410

Centisome position: 72.73

GC content: 39.59

Gene sequence:

>1278_bases
ATGCCCGCATCGTTAGTAGTAGGAACCCAATGGGGAGATGAAGGAAAGGCAAAGGTAATCGATTTTCTTTCCAAGGACAC
GGACATCATCGTTCGTTATCAAGGTGGAGCAAACGCGGGACATACGGTTGTAGTTCACGGAAAAAAATACGTGTTTCATT
TAGTTCCTTCGGGAGTAATCTACGACCAAACCATCTGTGTAATCGGGAACGGAGTTGTTTTAGATCCTCTTTTTTTCATA
GAAGAATGTGACCGCCTTCAAAAAGAAGGCTTTCCAGTCTTCGACAAACTCTTGTTAAGCGACGCTTGTCATCTTCTATT
TCCGTATCATTCTCAGATCGATTCCGCAAGAGAAACTACACTCAGTCAAGAGCACAAAATCGGAACCACTAAAAAAGGAA
TCGGGATCTGTTACGCGGATAAAATGATGAGGACCGGATTGCGAGTAGGAGATCTTTTAGACACTTCTTACCAAACCCGT
TTGAAACATTTAGTGGACGAAAAAAACAGAGAACTAGACAAACTCTACGGAATGCCTCCAGTCTCTTATAACGATATTAA
TGAGGGTTTAAAATTTTTTCTTTCTAAAGTTAAAAAGAATATTATCAATACTGCATATTATTTAGATACGGAACTAAAAA
AAGGAAAACGAGTTCTTTTGGAAGGAGCCCAAGGTACCGGTTTAGACGTAGATTTCGGAACCTATCCTTATGTAACCAGT
TCTAATCCCACAACGGGAGGAGCTTTAATTGGAACTGGAATTCCGTTTCAACATTTAAAACACGTAATTGGAATTACAAA
GGCATATACCACGAGAGTCGGAGAAGGCCCCTTCCCTACTGAACTTTTAGGAGAAGCTGGAGAAAAACTTCGTCAAAAAG
GAGGCGAATTTGGAGCGACTACGGGACGTCCCAGACGTTGCGGCTGGTTTGATGTAGAAATGCTAAAACATTCCGTTCGG
ATTAACGGAATCACTTCGATCGCTTTGACAAAAATAGATATTCTTTCCGACTATGATACAATTCCGGTTGCAACCGGATA
TAAACTAAATGGAAAAACTTTAGATTGTTTTCCATCTCAAGGTTTGGATAAAGTGGAAGTCATCTATGAAGAATTTCCAG
GATGGAAATCCGACATTTCAGGCATCTGCGAATTTCAAAAACTTCCAGAAAAATGTAAAAATTATATTTCTGCTTTAGAA
AAATGGATCGGAGTGAAAATCAATTTGGTTTCAACTGGTCCCGATAGAAAGGATACGATTCACGGAGATTCTTTTTAA

Upstream 100 bases:

>100_bases
GCACTCATGGTAAATACTTTAGAATCCTCTTTAAAAACTCCTCTTTTCAATCTTTCATCATGATACGTATTCATACCAAA
CATTCATTCGAGGAAAAATT

Downstream 100 bases:

>100_bases
GAATCGAAGTTTTTTTAATCGTTTTTGATTGACCCACGATACTCGATAAATATCGTGTATTTCGTAACGCTTCGAGTCGT
TAGCTCAGCTGGTAGAGCAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFI
EECDRLQKEGFPVFDKLLLSDACHLLFPYHSQIDSARETTLSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDTSYQTR
LKHLVDEKNRELDKLYGMPPVSYNDINEGLKFFLSKVKKNIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
SNPTTGGALIGTGIPFQHLKHVIGITKAYTTRVGEGPFPTELLGEAGEKLRQKGGEFGATTGRPRRCGWFDVEMLKHSVR
INGITSIALTKIDILSDYDTIPVATGYKLNGKTLDCFPSQGLDKVEVIYEEFPGWKSDISGICEFQKLPEKCKNYISALE
KWIGVKINLVSTGPDRKDTIHGDSF

Sequences:

>Translated_425_residues
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFI
EECDRLQKEGFPVFDKLLLSDACHLLFPYHSQIDSARETTLSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDTSYQTR
LKHLVDEKNRELDKLYGMPPVSYNDINEGLKFFLSKVKKNIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
SNPTTGGALIGTGIPFQHLKHVIGITKAYTTRVGEGPFPTELLGEAGEKLRQKGGEFGATTGRPRRCGWFDVEMLKHSVR
INGITSIALTKIDILSDYDTIPVATGYKLNGKTLDCFPSQGLDKVEVIYEEFPGWKSDISGICEFQKLPEKCKNYISALE
KWIGVKINLVSTGPDRKDTIHGDSF
>Mature_424_residues
PASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVIYDQTICVIGNGVVLDPLFFIE
ECDRLQKEGFPVFDKLLLSDACHLLFPYHSQIDSARETTLSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDTSYQTRL
KHLVDEKNRELDKLYGMPPVSYNDINEGLKFFLSKVKKNIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTSS
NPTTGGALIGTGIPFQHLKHVIGITKAYTTRVGEGPFPTELLGEAGEKLRQKGGEFGATTGRPRRCGWFDVEMLKHSVRI
NGITSIALTKIDILSDYDTIPVATGYKLNGKTLDCFPSQGLDKVEVIYEEFPGWKSDISGICEFQKLPEKCKNYISALEK
WIGVKINLVSTGPDRKDTIHGDSF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=429, Percent_Identity=45.4545454545455, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=42.7570093457944, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=44.1102756892231, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1790620, Length=420, Percent_Identity=43.8095238095238, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25146553, Length=430, Percent_Identity=44.6511627906977, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI25146555, Length=430, Percent_Identity=44.6511627906977, Blast_Score=357, Evalue=7e-99,
Organism=Saccharomyces cerevisiae, GI6324109, Length=435, Percent_Identity=42.9885057471264, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.8235294117647, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_LEPIC (Q72PA7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002492.1
- ProteinModelPortal:   Q72PA7
- SMR:   Q72PA7
- GeneID:   2772811
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12565
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK13787
- BioCyc:   LINT267671:LIC_12565-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47058; Mature: 46926

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVI
CCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YDQTICVIGNGVVLDPLFFIEECDRLQKEGFPVFDKLLLSDACHLLFPYHSQIDSARETT
ECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHH
LSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDTSYQTRLKHLVDEKNRELDKLYGMPP
HHHHHCCCCCCCCCEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
VSYNDINEGLKFFLSKVKKNIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCEEEEECCCCCCCEECCCCCCEEEC
SNPTTGGALIGTGIPFQHLKHVIGITKAYTTRVGEGPFPTELLGEAGEKLRQKGGEFGAT
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TGRPRRCGWFDVEMLKHSVRINGITSIALTKIDILSDYDTIPVATGYKLNGKTLDCFPSQ
CCCCCCCCCEEHHHHHHHHEECCEEEEHEEEEHHHCCCCCCCCCCCEEECCCEEECCCCC
GLDKVEVIYEEFPGWKSDISGICEFQKLPEKCKNYISALEKWIGVKINLVSTGPDRKDTI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
HGDSF
CCCCC
>Mature Secondary Structure 
PASLVVGTQWGDEGKAKVIDFLSKDTDIIVRYQGGANAGHTVVVHGKKYVFHLVPSGVI
CCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YDQTICVIGNGVVLDPLFFIEECDRLQKEGFPVFDKLLLSDACHLLFPYHSQIDSARETT
ECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCHHHHHHHHHHH
LSQEHKIGTTKKGIGICYADKMMRTGLRVGDLLDTSYQTRLKHLVDEKNRELDKLYGMPP
HHHHHCCCCCCCCCEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCC
VSYNDINEGLKFFLSKVKKNIINTAYYLDTELKKGKRVLLEGAQGTGLDVDFGTYPYVTS
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHCCCEEEEECCCCCCCEECCCCCCEEEC
SNPTTGGALIGTGIPFQHLKHVIGITKAYTTRVGEGPFPTELLGEAGEKLRQKGGEFGAT
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TGRPRRCGWFDVEMLKHSVRINGITSIALTKIDILSDYDTIPVATGYKLNGKTLDCFPSQ
CCCCCCCCCEEHHHHHHHHEECCEEEEHEEEEHHHCCCCCCCCCCCEEECCCEEECCCCC
GLDKVEVIYEEFPGWKSDISGICEFQKLPEKCKNYISALEKWIGVKINLVSTGPDRKDTI
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
HGDSF
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA