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The map label for this gene is hisZ

Identifier: 45658405

GI number: 45658405

Start: 3109787

End: 3110821

Strand: Direct

Name: hisZ

Synonym: LIC12564

Alternate gene names: 45658405

Gene position: 3109787-3110821 (Clockwise)

Preceding gene: 45658402

Following gene: 45658406

Centisome position: 72.71

GC content: 34.98

Gene sequence:

>1035_bases
ATGAATCAAAATCTTCCAGAACCTAATCAAAAAAAATGGATCCCAGACGGATTTCATTTTCTTGGCCCCGAAGATAGCAA
GTACAGAAGGACTTTACTTGAAACCATATCCGGTGTTCTTAAAAAAAAAGGATACTCGGAAGTATTTTTACCTGCATTCG
ATTATAGCTCCACTTTCATTCAAACGGTTTCGGCCCCGGATTCATCTTCCCTTTTCCGAATCAGAGATCTTTCCGGAAAT
GAAATTTCTCCCAGCATCGATCTGACCGTTCAAGCGGTCAAAGGAATGGCAGGTTTTTCTCACCAAAGAGAAAATCAAAA
CATCTTTTATATTGGAAGAGTTTTCAGAGAAAGTACAAAAGGAAGTGTAGCTCGTAAAGAAATACTACAAATTGGAGCGG
AATCGATCGGTGTTTCCGGAAAGGAAAACACATTCAAAATCTTAGAGGAACTGGACGAAATCATTTCTTTACTTCCTCTT
GAAAACAAACTGACTCTTGTTTTAGGAAACGTAAATCTGTTCCAATCTATCGTTCAAGAATTTGAACTCAAACAAAATGA
AATCGAGATTCTTTCTAAATTACTATATCAAAAAAACGTAAACGAGATCCAGAAAATTTTCGGAGAAAAAAAGAACCATA
CCGATTTGATTCGTTTGTTAAGCTCCTTGGTTTTAAATTTTGATCTAAATTCTTTGAAAAATTCTCTCAATATAAATTCA
CTTTCTAAGAATCTTCAAAAAAGCTTAAGTTCAATTCTTGAAGAAACATACTGGATTTTTAATTCTTGGGAATCTAAAAA
AAGAAGGATAGACCTCTGTATCGATTTTTCTCTTTTGAGAGATCTAAACTATTATACCGGTTTTGTTTTCCAAGGTTATC
TGCAAGATTCTCCCGATCCAGTTTTAACCGGAGGAACCTATGATCATCTCTACGAAATGTTTTCCGGGGTACAAAAAAAC
GCGAGTGGTTATGCACTCATGGTAAATACTTTAGAATCCTCTTTAAAAACTCCTCTTTTCAATCTTTCATCATGA

Upstream 100 bases:

>100_bases
CATTCGTTTGCGGGGGAATTGTCATTCTATTTTTTTAAAAAGGTTTGAAATCTGACGTCTGAGTTTTGAACTGTATTTTG
GAACTGAAGTTTTCCAAAAC

Downstream 100 bases:

>100_bases
TACGTATTCATACCAAACATTCATTCGAGGAAAAATTATGCCCGCATCGTTAGTAGTAGGAACCCAATGGGGAGATGAAG
GAAAGGCAAAGGTAATCGAT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNQNLPEPNQKKWIPDGFHFLGPEDSKYRRTLLETISGVLKKKGYSEVFLPAFDYSSTFIQTVSAPDSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRVFRESTKGSVARKEILQIGAESIGVSGKENTFKILEELDEIISLLPL
ENKLTLVLGNVNLFQSIVQEFELKQNEIEILSKLLYQKNVNEIQKIFGEKKNHTDLIRLLSSLVLNFDLNSLKNSLNINS
LSKNLQKSLSSILEETYWIFNSWESKKRRIDLCIDFSLLRDLNYYTGFVFQGYLQDSPDPVLTGGTYDHLYEMFSGVQKN
ASGYALMVNTLESSLKTPLFNLSS

Sequences:

>Translated_344_residues
MNQNLPEPNQKKWIPDGFHFLGPEDSKYRRTLLETISGVLKKKGYSEVFLPAFDYSSTFIQTVSAPDSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRVFRESTKGSVARKEILQIGAESIGVSGKENTFKILEELDEIISLLPL
ENKLTLVLGNVNLFQSIVQEFELKQNEIEILSKLLYQKNVNEIQKIFGEKKNHTDLIRLLSSLVLNFDLNSLKNSLNINS
LSKNLQKSLSSILEETYWIFNSWESKKRRIDLCIDFSLLRDLNYYTGFVFQGYLQDSPDPVLTGGTYDHLYEMFSGVQKN
ASGYALMVNTLESSLKTPLFNLSS
>Mature_344_residues
MNQNLPEPNQKKWIPDGFHFLGPEDSKYRRTLLETISGVLKKKGYSEVFLPAFDYSSTFIQTVSAPDSSSLFRIRDLSGN
EISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRVFRESTKGSVARKEILQIGAESIGVSGKENTFKILEELDEIISLLPL
ENKLTLVLGNVNLFQSIVQEFELKQNEIEILSKLLYQKNVNEIQKIFGEKKNHTDLIRLLSSLVLNFDLNSLKNSLNINS
LSKNLQKSLSSILEETYWIFNSWESKKRRIDLCIDFSLLRDLNYYTGFVFQGYLQDSPDPVLTGGTYDHLYEMFSGVQKN
ASGYALMVNTLESSLKTPLFNLSS

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_LEPIC (P62359)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002491.1
- ProteinModelPortal:   P62359
- GeneID:   2772186
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12564
- HOGENOM:   HBG529450
- OMA:   ENTSETH
- BioCyc:   LINT-130-01:LINT-130-01-002491-MONOMER
- BioCyc:   LINT267671:LIC_12564-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004516
- PANTHER:   PTHR11476

Pfam domain/function: NA

EC number: 6.1.1.21

Molecular weight: Translated: 39114; Mature: 39114

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQNLPEPNQKKWIPDGFHFLGPEDSKYRRTLLETISGVLKKKGYSEVFLPAFDYSSTFI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
QTVSAPDSSSLFRIRDLSGNEISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRVFRESTK
HEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCC
GSVARKEILQIGAESIGVSGKENTFKILEELDEIISLLPLENKLTLVLGNVNLFQSIVQE
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHH
FELKQNEIEILSKLLYQKNVNEIQKIFGEKKNHTDLIRLLSSLVLNFDLNSLKNSLNINS
HHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHH
LSKNLQKSLSSILEETYWIFNSWESKKRRIDLCIDFSLLRDLNYYTGFVFQGYLQDSPDP
HHHHHHHHHHHHHHHHHHHHHCHHHHCCEEHHEECHHHHHHHHHHHHHHEEECCCCCCCC
VLTGGTYDHLYEMFSGVQKNASGYALMVNTLESSLKTPLFNLSS
EEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNQNLPEPNQKKWIPDGFHFLGPEDSKYRRTLLETISGVLKKKGYSEVFLPAFDYSSTFI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
QTVSAPDSSSLFRIRDLSGNEISPSIDLTVQAVKGMAGFSHQRENQNIFYIGRVFRESTK
HEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCC
GSVARKEILQIGAESIGVSGKENTFKILEELDEIISLLPLENKLTLVLGNVNLFQSIVQE
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHH
FELKQNEIEILSKLLYQKNVNEIQKIFGEKKNHTDLIRLLSSLVLNFDLNSLKNSLNINS
HHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCHHH
LSKNLQKSLSSILEETYWIFNSWESKKRRIDLCIDFSLLRDLNYYTGFVFQGYLQDSPDP
HHHHHHHHHHHHHHHHHHHHHCHHHHCCEEHHEECHHHHHHHHHHHHHHEEECCCCCCCC
VLTGGTYDHLYEMFSGVQKNASGYALMVNTLESSLKTPLFNLSS
EEECCCHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA