Click here to switch to the map view.

The map label for this gene is lpdA

Identifier: 45658317

GI number: 45658317

Start: 2997856

End: 2999259

Strand: Reverse

Name: lpdA

Synonym: LIC12475

Alternate gene names: 45658317

Gene position: 2999259-2997856 (Counterclockwise)

Preceding gene: 45658318

Following gene: 45658316

Centisome position: 70.12

GC content: 45.23

Gene sequence:

>1404_bases
ATGTCAGCAGAATTTGACGTAGTCGTGATCGGCGCGGGACCGGGGGGCTACGTTTGTGCCATCCGCTGCGCGCAACTTGG
TTTTAAAACCGCAATCATCGAAAAAAGAAAAACCCTCGGAGGCACTTGTCTAAACGTGGGTTGTATTCCTTCTAAAGCGC
TTTTAGATTCTTCTGAAGAATATCATAAAACTTTGCATAAGCTGGAAGTTCACGGGATCAGTGTTGGAAAAGTGGATTTG
GATCTAAACAAGCTTATGAACCGTAAGGATCAGATTGTAAAGGAAGTCACGGATGGAGTAGACTTTCTTATGAACAAAAA
TAAGATCAAACGTTACGAAGGTTTTGGAAAGGTATTGTCTGCTGGAAAAGTAGAAGTCGCATTCAATGACGGAAAAAAGG
AAACTCTCTCCGCCAAACACATCGTAGTCGCTACTGGCTCGGTTCCGATTGATATTCCAGGTTTAACCGTGGACGGAAAA
AATATCATCACTTCCGATCACGCGATTGATGTTCGTAAACTTCCCAAAAAAATGATCATCATCGGAGCCGGAGTGATCGG
ACTTGAACTAGGATCTGTTTGGGGAAGACTCGGAACCTCTGTGACCGTGGTTGAATTTTTGCCCGGACTCATTTCCAATG
TAGATCGTCAGATGGGTTCGTTACTAGAACGTTCTCTTACTTCTCAAGGAATGGAATTTTTATTTGAACATAAGGTAAAA
GGTGCGACTACTACAAAGAACGGAGTGAAGGTTCAGATCGAAGATTCTAAAGGAGAGTCTAAGGATCTAGAGGCGGATGT
GGTTCTGGTCGCAGTTGGACGTAGACCTTTTCTGGAAGGAGTGGGACTGGAGGAAGTGGGTGTAGCGTTTACACAACGCA
AACGGATTCAAGTGGATGCACATTTTAAAACGTCCGTTCCCGGAATTTATGCAATCGGAGATGCGATTGATGGACCTATG
CTCGCACACAAAGCGGAAGAAGAAGGGGTCGCTCTTGCAGAACTTCTTGCCGGTCAATCCGGTCATGTCAATTACAACGC
AGTTCCTTACGTCATATATACTTGGCCGGAAATGGCTTGGGTAGGAAAGGGCGAAGAAGAATTGAAGGCTGCAGGAATTG
AATATAAAGTAGGAAAGTCGCTCTTTCGGCCCAATGCTCGTTCCAAAGCGATGAACGAAGCAGAAGGACAAGTCAAAATA
TTAGCGGATAAAAAAACGGATAAACTTTTAGGAGCTTTTGTATTTGGCCCAAGGGCTTCGGATATGGTGGCGGAATTAGC
GGTGGCTATGGAGTTCGGAGCTTCTGCAGAAGATATAGCGAGAAGTTTCCATGCACATCCGACCTTGTCGGAAGTGATTA
AAGAAGCGGCAATGGCGGTGGATAAGTGGGCGATTCACGCGTGA

Upstream 100 bases:

>100_bases
ATCGTAGATGGAAAGGAGGCGGTCACTTTCCTTGTAAAGGTTAAGGAAGCGATCGAAGATCCAAGTCGGCTTTTACTAGA
ACTTTAATGAAAGAGGAAGT

Downstream 100 bases:

>100_bases
ATCGTATTAGGTAGGTTTCAAAAATGAAGATAGAAAAACTCATGGCGCTTTATGGGGAGAACGGCGCTCTCCTCGAAGAA
CTTTATAATCAATATAAACT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSAEFDVVVIGAGPGGYVCAIRCAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKTLHKLEVHGISVGKVDL
DLNKLMNRKDQIVKEVTDGVDFLMNKNKIKRYEGFGKVLSAGKVEVAFNDGKKETLSAKHIVVATGSVPIDIPGLTVDGK
NIITSDHAIDVRKLPKKMIIIGAGVIGLELGSVWGRLGTSVTVVEFLPGLISNVDRQMGSLLERSLTSQGMEFLFEHKVK
GATTTKNGVKVQIEDSKGESKDLEADVVLVAVGRRPFLEGVGLEEVGVAFTQRKRIQVDAHFKTSVPGIYAIGDAIDGPM
LAHKAEEEGVALAELLAGQSGHVNYNAVPYVIYTWPEMAWVGKGEEELKAAGIEYKVGKSLFRPNARSKAMNEAEGQVKI
LADKKTDKLLGAFVFGPRASDMVAELAVAMEFGASAEDIARSFHAHPTLSEVIKEAAMAVDKWAIHA

Sequences:

>Translated_467_residues
MSAEFDVVVIGAGPGGYVCAIRCAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKTLHKLEVHGISVGKVDL
DLNKLMNRKDQIVKEVTDGVDFLMNKNKIKRYEGFGKVLSAGKVEVAFNDGKKETLSAKHIVVATGSVPIDIPGLTVDGK
NIITSDHAIDVRKLPKKMIIIGAGVIGLELGSVWGRLGTSVTVVEFLPGLISNVDRQMGSLLERSLTSQGMEFLFEHKVK
GATTTKNGVKVQIEDSKGESKDLEADVVLVAVGRRPFLEGVGLEEVGVAFTQRKRIQVDAHFKTSVPGIYAIGDAIDGPM
LAHKAEEEGVALAELLAGQSGHVNYNAVPYVIYTWPEMAWVGKGEEELKAAGIEYKVGKSLFRPNARSKAMNEAEGQVKI
LADKKTDKLLGAFVFGPRASDMVAELAVAMEFGASAEDIARSFHAHPTLSEVIKEAAMAVDKWAIHA
>Mature_466_residues
SAEFDVVVIGAGPGGYVCAIRCAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEEYHKTLHKLEVHGISVGKVDLD
LNKLMNRKDQIVKEVTDGVDFLMNKNKIKRYEGFGKVLSAGKVEVAFNDGKKETLSAKHIVVATGSVPIDIPGLTVDGKN
IITSDHAIDVRKLPKKMIIIGAGVIGLELGSVWGRLGTSVTVVEFLPGLISNVDRQMGSLLERSLTSQGMEFLFEHKVKG
ATTTKNGVKVQIEDSKGESKDLEADVVLVAVGRRPFLEGVGLEEVGVAFTQRKRIQVDAHFKTSVPGIYAIGDAIDGPML
AHKAEEEGVALAELLAGQSGHVNYNAVPYVIYTWPEMAWVGKGEEELKAAGIEYKVGKSLFRPNARSKAMNEAEGQVKIL
ADKKTDKLLGAFVFGPRASDMVAELAVAMEFGASAEDIARSFHAHPTLSEVIKEAAMAVDKWAIHA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=51.4161220043573, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.5097192224622, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=31.2087912087912, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI148277065, Length=455, Percent_Identity=26.8131868131868, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519430, Length=455, Percent_Identity=26.8131868131868, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519428, Length=455, Percent_Identity=26.8131868131868, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI33519426, Length=455, Percent_Identity=26.8131868131868, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI148277071, Length=455, Percent_Identity=26.5934065934066, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI291045266, Length=442, Percent_Identity=26.4705882352941, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI291045268, Length=436, Percent_Identity=25.2293577981651, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=36.3834422657952, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=27.3318872017354, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=25.9656652360515, Blast_Score=158, Evalue=9e-40,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=27.1493212669683, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789065, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=50.9846827133479, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=29.1060291060291, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=26.0416666666667, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=27.2331154684096, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=27.2331154684096, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=47.7987421383648, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=32.4152542372881, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=28.997867803838, Blast_Score=157, Evalue=5e-39,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=50.9803921568627, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=29.0794979079498, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=29.0794979079498, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=29.0794979079498, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=29.4736842105263, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50218; Mature: 50086

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEFDVVVIGAGPGGYVCAIRCAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEE
CCCCEEEEEEECCCCCCEEEHHHHHHCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHH
YHKTLHKLEVHGISVGKVDLDLNKLMNRKDQIVKEVTDGVDFLMNKNKIKRYEGFGKVLS
HHHHHHHHEECCEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
AGKVEVAFNDGKKETLSAKHIVVATGSVPIDIPGLTVDGKNIITSDHAIDVRKLPKKMII
CCEEEEEECCCCCCCCCCCEEEEEECCCEEECCCEEECCCCEEECCCCCCHHHCCCEEEE
IGAGVIGLELGSVWGRLGTSVTVVEFLPGLISNVDRQMGSLLERSLTSQGMEFLFEHKVK
EECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GATTTKNGVKVQIEDSKGESKDLEADVVLVAVGRRPFLEGVGLEEVGVAFTQRKRIQVDA
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEE
HFKTSVPGIYAIGDAIDGPMLAHKAEEEGVALAELLAGQSGHVNYNAVPYVIYTWPEMAW
EEECCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCEEE
VGKGEEELKAAGIEYKVGKSLFRPNARSKAMNEAEGQVKILADKKTDKLLGAFVFGPRAS
CCCCHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHEEECCCHH
DMVAELAVAMEFGASAEDIARSFHAHPTLSEVIKEAAMAVDKWAIHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAEFDVVVIGAGPGGYVCAIRCAQLGFKTAIIEKRKTLGGTCLNVGCIPSKALLDSSEE
CCCEEEEEEECCCCCCEEEHHHHHHCHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHH
YHKTLHKLEVHGISVGKVDLDLNKLMNRKDQIVKEVTDGVDFLMNKNKIKRYEGFGKVLS
HHHHHHHHEECCEEEEEEECCHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
AGKVEVAFNDGKKETLSAKHIVVATGSVPIDIPGLTVDGKNIITSDHAIDVRKLPKKMII
CCEEEEEECCCCCCCCCCCEEEEEECCCEEECCCEEECCCCEEECCCCCCHHHCCCEEEE
IGAGVIGLELGSVWGRLGTSVTVVEFLPGLISNVDRQMGSLLERSLTSQGMEFLFEHKVK
EECCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GATTTKNGVKVQIEDSKGESKDLEADVVLVAVGRRPFLEGVGLEEVGVAFTQRKRIQVDA
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEE
HFKTSVPGIYAIGDAIDGPMLAHKAEEEGVALAELLAGQSGHVNYNAVPYVIYTWPEMAW
EEECCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCCEEE
VGKGEEELKAAGIEYKVGKSLFRPNARSKAMNEAEGQVKILADKKTDKLLGAFVFGPRAS
CCCCHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCCCCEEEEECCCCHHHHHHEEECCCHH
DMVAELAVAMEFGASAEDIARSFHAHPTLSEVIKEAAMAVDKWAIHA
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]