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The map label for this gene is sucB

Identifier: 45658318

GI number: 45658318

Start: 2999273

End: 3000538

Strand: Reverse

Name: sucB

Synonym: LIC12476

Alternate gene names: 45658318

Gene position: 3000538-2999273 (Counterclockwise)

Preceding gene: 45658319

Following gene: 45658317

Centisome position: 70.15

GC content: 43.44

Gene sequence:

>1266_bases
ATGTCTGTAGAAATTAAGGTCCCCGAAATGGGGGAATCCATTACGGAAGCTACGATTGCAAACTGGGTAAAGAAAGAAGG
AGAATCTGTAAAACAAGACGAGATCTTGTTAGAACTGGAAACCGATAAGGCGACCATGGAAGTTCCGGCTCCTTCTTCGG
GTGTTCTTCAGAAAATTCATAAAAAGGCTGGAGAAACCGTAAAAGTAAAGGAGATCATCGGACTGATTGACTCTTCAGCA
ACTGCTTCTTCTCCTTCTCCTTCCGCTTCTGCTCCAACAAATTCAGCACAAACGACTCAAACATCAGGAAACGGAACCAT
AAACGAAACTCTTCCGCCTGCGGTCCGTAAGTTGATCGAAGACAACGGACTCAATCCGGCATCGATTACTGGTTCCGGTA
AAAACGGACAAATCACAAAAGAAGACGTATTAAAAGCAATTGAAACAAAAGCGACGTCGTCAGTTTCAAACGCTTCCGTA
AATGTAGGAACTCCAGCGGCTGTCAAAGCGACGCTGACTTTACCTGAAATTCCGAAAGCGGTTCCGGCTGCGAGAAGAAC
GGATCTACCAAGAGAAAACGTAGTTCCTATGACTCGACTTCGTAAGGTAATTGCAGAAAGACTTGTATCGGCACAACACA
ACGCTGCAATTCTGACCACGTTTAACGAAGTAGATATGAGTGCCGTAATGGAACTCAGAAATCGATATAAGGATAAATTT
AAAGAAGCGCATAACGTAAGTCTTGGATTTATGAGCTTTTTTACTAAGGCTGCGATCCATGCGCTAAAAACAATTCCGGC
GATCAATGCAGAAATCCGTGGAAGTGATATTGTTTACAAAAATTATTATGACATAGGGGTCGCTGTTGGAGGACCGAAGG
GACTGGTCGTTCCTGTCGTAAGAGACGCGGACTTGCTCAGTTTTGCAGGAGTAGAACAAGAGATTATAAGACTTGCGAAT
CGGGTCAAAGACGGAAAAATTGAACTTGCTGAAATGGAAGGTGGGACGTTTACGATTTCCAATGGGGGAATCTACGGTTC
TATGATGTCCACTCCAATTCTCAATCCCCCACAAAGTGGAATTTTAGGACTTCATAATATAGTAAAACGTGCTGTGGTGG
TAAACGATCAGATCGTGATTCGTCCGATGATGTATCTTGCACTTTCCTACGATCATAGAATCGTAGATGGAAAGGAGGCG
GTCACTTTCCTTGTAAAGGTTAAGGAAGCGATCGAAGATCCAAGTCGGCTTTTACTAGAACTTTAA

Upstream 100 bases:

>100_bases
CAGAACAGAATAATTTCGTTTTGGAGAATTTCGTTTACAGAATTTTTTCATTTAGAAGGATTGAAACCGAGATGGCGTTG
TCTCTCGAAAGGAATCATTC

Downstream 100 bases:

>100_bases
TGAAAGAGGAAGTATGTCAGCAGAATTTGACGTAGTCGTGATCGGCGCGGGACCGGGGGGCTACGTTTGTGCCATCCGCT
GCGCGCAACTTGGTTTTAAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSVEIKVPEMGESITEATIANWVKKEGESVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGETVKVKEIIGLIDSSA
TASSPSPSASAPTNSAQTTQTSGNGTINETLPPAVRKLIEDNGLNPASITGSGKNGQITKEDVLKAIETKATSSVSNASV
NVGTPAAVKATLTLPEIPKAVPAARRTDLPRENVVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDKF
KEAHNVSLGFMSFFTKAAIHALKTIPAINAEIRGSDIVYKNYYDIGVAVGGPKGLVVPVVRDADLLSFAGVEQEIIRLAN
RVKDGKIELAEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEA
VTFLVKVKEAIEDPSRLLLEL

Sequences:

>Translated_421_residues
MSVEIKVPEMGESITEATIANWVKKEGESVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGETVKVKEIIGLIDSSA
TASSPSPSASAPTNSAQTTQTSGNGTINETLPPAVRKLIEDNGLNPASITGSGKNGQITKEDVLKAIETKATSSVSNASV
NVGTPAAVKATLTLPEIPKAVPAARRTDLPRENVVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDKF
KEAHNVSLGFMSFFTKAAIHALKTIPAINAEIRGSDIVYKNYYDIGVAVGGPKGLVVPVVRDADLLSFAGVEQEIIRLAN
RVKDGKIELAEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEA
VTFLVKVKEAIEDPSRLLLEL
>Mature_420_residues
SVEIKVPEMGESITEATIANWVKKEGESVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGETVKVKEIIGLIDSSAT
ASSPSPSASAPTNSAQTTQTSGNGTINETLPPAVRKLIEDNGLNPASITGSGKNGQITKEDVLKAIETKATSSVSNASVN
VGTPAAVKATLTLPEIPKAVPAARRTDLPRENVVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDKFK
EAHNVSLGFMSFFTKAAIHALKTIPAINAEIRGSDIVYKNYYDIGVAVGGPKGLVVPVVRDADLLSFAGVEQEIIRLANR
VKDGKIELAEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAV
TFLVKVKEAIEDPSRLLLEL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=50.4065040650407, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=28.9760348583878, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI110671329, Length=431, Percent_Identity=28.538283062645, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=28.9760348583878, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI31711992, Length=449, Percent_Identity=26.5033407572383, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=31.875, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786946, Length=422, Percent_Identity=44.7867298578199, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1786305, Length=438, Percent_Identity=26.9406392694064, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=40.6175771971496, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17537937, Length=431, Percent_Identity=26.6821345707657, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=458, Percent_Identity=27.7292576419214, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=29.6774193548387, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=44.7619047619048, Blast_Score=324, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=458, Percent_Identity=25.764192139738, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=52.2321428571429, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=28.0182232346241, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45199; Mature: 45067

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIKVPEMGESITEATIANWVKKEGESVKQDEILLELETDKATMEVPAPSSGVLQKIH
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KKAGETVKVKEIIGLIDSSATASSPSPSASAPTNSAQTTQTSGNGTINETLPPAVRKLIE
HHHCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHH
DNGLNPASITGSGKNGQITKEDVLKAIETKATSSVSNASVNVGTPAAVKATLTLPEIPKA
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCC
VPAARRTDLPRENVVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDKF
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
KEAHNVSLGFMSFFTKAAIHALKTIPAINAEIRGSDIVYKNYYDIGVAVGGPKGLVVPVV
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEEEEEEEEECCCCCEEEEEE
RDADLLSFAGVEQEIIRLANRVKDGKIELAEMEGGTFTISNGGIYGSMMSTPILNPPQSG
CCCCHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEHHHCCCCCCCCCCC
ILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVKEAIEDPSRLLLE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHC
L
C
>Mature Secondary Structure 
SVEIKVPEMGESITEATIANWVKKEGESVKQDEILLELETDKATMEVPAPSSGVLQKIH
CEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KKAGETVKVKEIIGLIDSSATASSPSPSASAPTNSAQTTQTSGNGTINETLPPAVRKLIE
HHHCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHH
DNGLNPASITGSGKNGQITKEDVLKAIETKATSSVSNASVNVGTPAAVKATLTLPEIPKA
HCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEECCCCCCC
VPAARRTDLPRENVVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDKF
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHH
KEAHNVSLGFMSFFTKAAIHALKTIPAINAEIRGSDIVYKNYYDIGVAVGGPKGLVVPVV
HHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEEEEEEEEECCCCCEEEEEE
RDADLLSFAGVEQEIIRLANRVKDGKIELAEMEGGTFTISNGGIYGSMMSTPILNPPQSG
CCCCHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCEEEHHHCCCCCCCCCCC
ILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVKEAIEDPSRLLLE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]