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The map label for this gene is fcl

Identifier: 45658054

GI number: 45658054

Start: 2658241

End: 2659185

Strand: Reverse

Name: fcl

Synonym: LIC12205

Alternate gene names: 45658054

Gene position: 2659185-2658241 (Counterclockwise)

Preceding gene: 45658055

Following gene: 45658053

Centisome position: 62.17

GC content: 39.05

Gene sequence:

>945_bases
ATGGAAAAGGAATCAAAGATTTATATCGCCGGTCATAAAGGACTTGTAGGTTCTGCGATTGAACGCGTTTTAAAAAAAGA
AGGTTACGAAAACATTTTAGGTAAGACTCATGCTGAATTGGATCTTACGGAACAATCTAAAGTGAATGAGTTCTTTGAGG
TGAATCGTCCCGAATATGTTTTTTTGGCTGCTGCAAAGGTGGGCGGAATTCACGCTAATAATACTTACCCAGCCGAATTC
ATTTTTTCTAATATTCAGATTCAAAATAATGTTATAGACGCGTGTTATCGATTTAAAACAAAAAAACTTCTTTTTCTAGG
TTCTTCCTGTATTTATCCTAAGTTTGCTAAACAACCAATGGATGAAAGTCAATTGTTGGACGGTAAATTGGAACCGACTA
ACGAACCTTATGCGGTCGCTAAAATTGCGGGGATCGTAATGTGCCAAAGTTATAATCGTCAATACGGCACAAATTTTATT
TCCGTCATGCCAACTAATCTCTACGGTCCTGGGGATAATTATCATCCTGAAAATTCTCATGTTCTTCCCGCCTTGATCCG
TCGTTTTTATGAAGCTAAAATAAAAAACCTTCCAGAAGTTGTAGTATGGGGGACTGGAAAACCGTTGAGAGAATTTTTAT
ATTCCGATGACATGGGACACGCCTGCGTTTTTTTAATGAAAAATTACGATGTGACCGGAGATCCGAAAGGGGGAGAGCAC
GTCAATGTTGGAAGTGGCATTGAAGTAAGTATTCGTGAACTTGCCGAAACCATTAAGGAAGTAGTAGGGTATCAAGGACT
TTTGACTTTCGACCTTACTAAACCGGACGGAACTCCTCGCAAACTTTTGGACGTTTCTAAGCTTCATAAAATGGGGTGGA
AACATCAGGTGGAATTGAAAGAAGGGATTCGGTTGGCGTTTGAGGATTATAAAACGGTAATATGA

Upstream 100 bases:

>100_bases
CGTATTACCGTACGTTATCTAAAATTGAAGATGCAAGAATATGAAGAGATTCAAAAAGAAATCGAGGAGTTGAAAAAGGA
AATCGGAGAACAATAAGGCG

Downstream 100 bases:

>100_bases
AGGACGTATTAACCATTTGATTTTAGTAATAGGTTCCGGCATAGTTGGAAGTTGGATCGCTTATCAAATTTCTAAGAATA
CCCAAGCAGATGTTTATTTA

Product: GDP-l-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEKESKIYIAGHKGLVGSAIERVLKKEGYENILGKTHAELDLTEQSKVNEFFEVNRPEYVFLAAAKVGGIHANNTYPAEF
IFSNIQIQNNVIDACYRFKTKKLLFLGSSCIYPKFAKQPMDESQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKNLPEVVVWGTGKPLREFLYSDDMGHACVFLMKNYDVTGDPKGGEH
VNVGSGIEVSIRELAETIKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDYKTVI

Sequences:

>Translated_314_residues
MEKESKIYIAGHKGLVGSAIERVLKKEGYENILGKTHAELDLTEQSKVNEFFEVNRPEYVFLAAAKVGGIHANNTYPAEF
IFSNIQIQNNVIDACYRFKTKKLLFLGSSCIYPKFAKQPMDESQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKNLPEVVVWGTGKPLREFLYSDDMGHACVFLMKNYDVTGDPKGGEH
VNVGSGIEVSIRELAETIKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDYKTVI
>Mature_314_residues
MEKESKIYIAGHKGLVGSAIERVLKKEGYENILGKTHAELDLTEQSKVNEFFEVNRPEYVFLAAAKVGGIHANNTYPAEF
IFSNIQIQNNVIDACYRFKTKKLLFLGSSCIYPKFAKQPMDESQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTNFI
SVMPTNLYGPGDNYHPENSHVLPALIRRFYEAKIKNLPEVVVWGTGKPLREFLYSDDMGHACVFLMKNYDVTGDPKGGEH
VNVGSGIEVSIRELAETIKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELKEGIRLAFEDYKTVI

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=286, Percent_Identity=34.6153846153846, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI7657641, Length=301, Percent_Identity=26.2458471760797, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1788365, Length=309, Percent_Identity=55.9870550161812, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=25.0696378830084, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788353, Length=351, Percent_Identity=24.5014245014245, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=314, Percent_Identity=31.2101910828025, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922778, Length=292, Percent_Identity=31.8493150684932, Blast_Score=155, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 35389; Mature: 35389

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKESKIYIAGHKGLVGSAIERVLKKEGYENILGKTHAELDLTEQSKVNEFFEVNRPEYV
CCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPAEFIFSNIQIQNNVIDACYRFKTKKLLFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHEEEEEEEHHHHHHHHHHHHCEEEEECCCCCCCHHHCCCC
DESQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTNFISVMPTNLYGPGDNYHPENSH
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VLPALIRRFYEAKIKNLPEVVVWGTGKPLREFLYSDDMGHACVFLMKNYDVTGDPKGGEH
CHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCE
VNVGSGIEVSIRELAETIKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELK
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
EGIRLAFEDYKTVI
HCHHHHHHHHHHCC
>Mature Secondary Structure
MEKESKIYIAGHKGLVGSAIERVLKKEGYENILGKTHAELDLTEQSKVNEFFEVNRPEYV
CCCCCEEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCEECCHHHHHHHHHHCCCCCEE
FLAAAKVGGIHANNTYPAEFIFSNIQIQNNVIDACYRFKTKKLLFLGSSCIYPKFAKQPM
EEEEEEECCEECCCCCCHHHHEEEEEEEHHHHHHHHHHHHCEEEEECCCCCCCHHHCCCC
DESQLLDGKLEPTNEPYAVAKIAGIVMCQSYNRQYGTNFISVMPTNLYGPGDNYHPENSH
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
VLPALIRRFYEAKIKNLPEVVVWGTGKPLREFLYSDDMGHACVFLMKNYDVTGDPKGGEH
CHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCE
VNVGSGIEVSIRELAETIKEVVGYQGLLTFDLTKPDGTPRKLLDVSKLHKMGWKHQVELK
EECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
EGIRLAFEDYKTVI
HCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]