Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 45658048

GI number: 45658048

Start: 2652237

End: 2653229

Strand: Reverse

Name: galE [C]

Synonym: LIC12199

Alternate gene names: 45658048

Gene position: 2653229-2652237 (Counterclockwise)

Preceding gene: 45658049

Following gene: 45658047

Centisome position: 62.03

GC content: 36.56

Gene sequence:

>993_bases
ATGAATCCAGATATTAAAAGTATCTATATAACAGGCGGGGCCGGTTACGTTGGGGCCATGCTTGTGCCCCGCCTTCTTTC
GGAGGGTTATAAAGTAACGGTGCTTGATCTTATGATCTATGGAGAAGATGTACTGAAAGAACATCCTAACCTAACGAAGA
TTCAAGGAGATATTCGAGATCAAAATGTCTTGAATCAAACAATTCCAGGCCATGATTCGGTGATTCATCTTGCATGTATT
TCCAACGATCCTAGCTTTGAATTGAATCCGAATTTAGGAAAATCAATCAATTTAGACGCATTCCGACCTCTTGTTGAAAT
TAGCAAAAAACACTCTGTCAAAAGATTTATTTATGCTTCTTCTTCTTCCGTTTATGGAATTAAAGATGAACCGAATGTTA
CGGAAGATTTTTCTTTAGAGCCGCTCACGGATTATTCCAAATTTAAAGCAGATTGCGAAAAAATATTAAACGAATATCAA
ACCGATGATTTTACGACGATTACAATAAGACCCGCGACAGTTTGCGGTTATTCGCCTAGACAAAGGTTGGATGTTGTAGT
AAATATCTTGACTAATCTAGCATATCACAAACGTGAAATCTCCGTATTTGGAGGAGCACAACTTCGTCCGAACATTCATA
TCGACGATATGGTGGATGCGTATTTGGTTTTATTACGCGCTCCAAAAGAAAAGGTAGCTGGAGAAATATTTAACGCAGGT
TACTTGAACTTTACAGTTTCTGAAATTGCTAATATGGTGAAAGAAGTTGTCGGAGAAGATGTAAAGTTAGTTACAACGCC
TACAAACGATAATCGATCCTACCATATTTCTTCTGATAAAATATTCAATAAGTTAGGGTTTCGTGCAAATCGTTCTATTA
AGTTGGCGGCAGAAGATTTAAAGAAAGCTTTTGATTCTGGTCTTCTTCCAAATTCTCTGACCGATGAAAAATATTTTAAT
ATAAAACGTATGCAATCTATAAGTTTAAGGTAA

Upstream 100 bases:

>100_bases
CGATTGGTGCTTGTATGGCGGCGCTTGCCGTTCAAACAATTGGAAATATACCCGTTTCTCATCAGAAACTTGAAAGCTAT
ATTAAGAATTTGAGGTAAAT

Downstream 100 bases:

>100_bases
GTAAAATGCAAGTAAGATATTCATATTTAAAACAACAATTTGAAAATTGCGATGATCTTTGGGATGAATTAAAAAGGTTT
GTTCCGACAGGAGATTTTAC

Product: UDP-glucose 4-epimerase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNPDIKSIYITGGAGYVGAMLVPRLLSEGYKVTVLDLMIYGEDVLKEHPNLTKIQGDIRDQNVLNQTIPGHDSVIHLACI
SNDPSFELNPNLGKSINLDAFRPLVEISKKHSVKRFIYASSSSVYGIKDEPNVTEDFSLEPLTDYSKFKADCEKILNEYQ
TDDFTTITIRPATVCGYSPRQRLDVVVNILTNLAYHKREISVFGGAQLRPNIHIDDMVDAYLVLLRAPKEKVAGEIFNAG
YLNFTVSEIANMVKEVVGEDVKLVTTPTNDNRSYHISSDKIFNKLGFRANRSIKLAAEDLKKAFDSGLLPNSLTDEKYFN
IKRMQSISLR

Sequences:

>Translated_330_residues
MNPDIKSIYITGGAGYVGAMLVPRLLSEGYKVTVLDLMIYGEDVLKEHPNLTKIQGDIRDQNVLNQTIPGHDSVIHLACI
SNDPSFELNPNLGKSINLDAFRPLVEISKKHSVKRFIYASSSSVYGIKDEPNVTEDFSLEPLTDYSKFKADCEKILNEYQ
TDDFTTITIRPATVCGYSPRQRLDVVVNILTNLAYHKREISVFGGAQLRPNIHIDDMVDAYLVLLRAPKEKVAGEIFNAG
YLNFTVSEIANMVKEVVGEDVKLVTTPTNDNRSYHISSDKIFNKLGFRANRSIKLAAEDLKKAFDSGLLPNSLTDEKYFN
IKRMQSISLR
>Mature_330_residues
MNPDIKSIYITGGAGYVGAMLVPRLLSEGYKVTVLDLMIYGEDVLKEHPNLTKIQGDIRDQNVLNQTIPGHDSVIHLACI
SNDPSFELNPNLGKSINLDAFRPLVEISKKHSVKRFIYASSSSVYGIKDEPNVTEDFSLEPLTDYSKFKADCEKILNEYQ
TDDFTTITIRPATVCGYSPRQRLDVVVNILTNLAYHKREISVFGGAQLRPNIHIDDMVDAYLVLLRAPKEKVAGEIFNAG
YLNFTVSEIANMVKEVVGEDVKLVTTPTNDNRSYHISSDKIFNKLGFRANRSIKLAAEDLKKAFDSGLLPNSLTDEKYFN
IKRMQSISLR

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI7657641, Length=324, Percent_Identity=28.7037037037037, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI42516563, Length=265, Percent_Identity=25.6603773584906, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786974, Length=326, Percent_Identity=25.1533742331288, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=334, Percent_Identity=24.5508982035928, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71982035, Length=334, Percent_Identity=24.251497005988, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=237, Percent_Identity=28.2700421940928, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17570557, Length=173, Percent_Identity=31.7919075144509, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=253, Percent_Identity=25.6916996047431, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356223, Length=263, Percent_Identity=26.615969581749, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 36999; Mature: 36999

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPDIKSIYITGGAGYVGAMLVPRLLSEGYKVTVLDLMIYGEDVLKEHPNLTKIQGDIRD
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHCCCCEEEECCCCH
QNVLNQTIPGHDSVIHLACISNDPSFELNPNLGKSINLDAFRPLVEISKKHSVKRFIYAS
HHHHHCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
SSSVYGIKDEPNVTEDFSLEPLTDYSKFKADCEKILNEYQTDDFTTITIRPATVCGYSPR
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCHH
QRLDVVVNILTNLAYHKREISVFGGAQLRPNIHIDDMVDAYLVLLRAPKEKVAGEIFNAG
HHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
YLNFTVSEIANMVKEVVGEDVKLVTTPTNDNRSYHISSDKIFNKLGFRANRSIKLAAEDL
EEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCCEEEEEHHHH
KKAFDSGLLPNSLTDEKYFNIKRMQSISLR
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPDIKSIYITGGAGYVGAMLVPRLLSEGYKVTVLDLMIYGEDVLKEHPNLTKIQGDIRD
CCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHHHCCCCEEEECCCCH
QNVLNQTIPGHDSVIHLACISNDPSFELNPNLGKSINLDAFRPLVEISKKHSVKRFIYAS
HHHHHCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEC
SSSVYGIKDEPNVTEDFSLEPLTDYSKFKADCEKILNEYQTDDFTTITIRPATVCGYSPR
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCHH
QRLDVVVNILTNLAYHKREISVFGGAQLRPNIHIDDMVDAYLVLLRAPKEKVAGEIFNAG
HHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
YLNFTVSEIANMVKEVVGEDVKLVTTPTNDNRSYHISSDKIFNKLGFRANRSIKLAAEDL
EEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHHCCCCCCEEEEEHHHH
KKAFDSGLLPNSLTDEKYFNIKRMQSISLR
HHHHHCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]