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The map label for this gene is rfbF [H]

Identifier: 45658000

GI number: 45658000

Start: 2601148

End: 2601915

Strand: Reverse

Name: rfbF [H]

Synonym: LIC12152

Alternate gene names: 45658000

Gene position: 2601915-2601148 (Counterclockwise)

Preceding gene: 45658001

Following gene: 45657999

Centisome position: 60.83

GC content: 40.49

Gene sequence:

>768_bases
ATGAAAACTGTAATACTCTGTGGAGGACTAGGAACTCGTTTGAGCGAGGAGACTACGGTGAAACCGAAGCCGATGGTGGA
AATTGCCGGGAAGCCAATACTATGGCATATTATGAAAATTTATGAATATCATGGATTCGGAAAGTTTCTTTTAGCTTTGG
GTTATAAAGGAGAAGTGATCAAAGATTACTTTTTAAACTACCACGCGAGAATGAGCGACCTCACTGTAAGTCTAAAATCC
GGTATTATAGAATATTCTAATCCGACAGCGGAAGATTGGGAAGTTGATTTAGTGGATACTGGCGCTTTAACTATGACGGG
GGGAAGGTTGTTACGACTCAAAGACCAGCTTTCAAAAGAAACTTTCATGGTCACCTATGGAGATGGTGTTGCGAATGTTG
ATATAAAGAAACTCGTAAGTTTTCACAAATCTCACGGAAAACTGGCGACTGTAACTGCGGTTCGTCCTCCGGTTCGATTT
GGAGAGTTATCTATTTCTGGAGATCAAGTTATTCAGTTCCAAGAAAAACCGCAAGCTGAAGAAGGTTGGATTAATGGAGG
CTTTTTTGTTTTTGAACCGGAAGTTCTAAATTACATCCAAGACGAATCTACGATGTTAGAACGGTCTCCTTTAGAAACTC
TTGCAAAGGCTGGTCAATTGATGGCGTTTCATCACGCCGGGTATTGGCAGTGTATGGATACTCTTCGAGATAAACATACT
TTGGAAGAACTCTGGAACCAAAATAAAGCGCCTTGGAAATTTAATTAA

Upstream 100 bases:

>100_bases
GACTTTAGTATTTCGGTAAAAGAAAGAAAGGCTTTTTTTTGTTTAGATCCAAATTTAACCAAATGGAAAAAACTTTTAAG
AATCGAGTAAGGTCGGAAAA

Downstream 100 bases:

>100_bases
GTATGTTTCAAAATATCTATAAAAATAAAAAGGTTCTGGTCACTGGACATACCGGTTTCAAAGGGTCTTGGTTAGTTATT
TGGTTACAGTCTTTAGGTGC

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKTVILCGGLGTRLSEETTVKPKPMVEIAGKPILWHIMKIYEYHGFGKFLLALGYKGEVIKDYFLNYHARMSDLTVSLKS
GIIEYSNPTAEDWEVDLVDTGALTMTGGRLLRLKDQLSKETFMVTYGDGVANVDIKKLVSFHKSHGKLATVTAVRPPVRF
GELSISGDQVIQFQEKPQAEEGWINGGFFVFEPEVLNYIQDESTMLERSPLETLAKAGQLMAFHHAGYWQCMDTLRDKHT
LEELWNQNKAPWKFN

Sequences:

>Translated_255_residues
MKTVILCGGLGTRLSEETTVKPKPMVEIAGKPILWHIMKIYEYHGFGKFLLALGYKGEVIKDYFLNYHARMSDLTVSLKS
GIIEYSNPTAEDWEVDLVDTGALTMTGGRLLRLKDQLSKETFMVTYGDGVANVDIKKLVSFHKSHGKLATVTAVRPPVRF
GELSISGDQVIQFQEKPQAEEGWINGGFFVFEPEVLNYIQDESTMLERSPLETLAKAGQLMAFHHAGYWQCMDTLRDKHT
LEELWNQNKAPWKFN
>Mature_255_residues
MKTVILCGGLGTRLSEETTVKPKPMVEIAGKPILWHIMKIYEYHGFGKFLLALGYKGEVIKDYFLNYHARMSDLTVSLKS
GIIEYSNPTAEDWEVDLVDTGALTMTGGRLLRLKDQLSKETFMVTYGDGVANVDIKKLVSFHKSHGKLATVTAVRPPVRF
GELSISGDQVIQFQEKPQAEEGWINGGFFVFEPEVLNYIQDESTMLERSPLETLAKAGQLMAFHHAGYWQCMDTLRDKHT
LEELWNQNKAPWKFN

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=236, Percent_Identity=29.6610169491525, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI11761619, Length=248, Percent_Identity=29.8387096774194, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=31.0483870967742, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320148, Length=246, Percent_Identity=30.0813008130081, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355443, Length=242, Percent_Identity=30.9917355371901, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24644084, Length=242, Percent_Identity=30.9917355371901, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28894; Mature: 28894

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVILCGGLGTRLSEETTVKPKPMVEIAGKPILWHIMKIYEYHGFGKFLLALGYKGEVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHEEHEECCCCCHH
KDYFLNYHARMSDLTVSLKSGIIEYSNPTAEDWEVDLVDTGALTMTGGRLLRLKDQLSKE
HHHHHHHHCHHHHEEEEHHHCEEECCCCCCCCEEEEEEECCEEEECCCEEEEEHHHCCCC
TFMVTYGDGVANVDIKKLVSFHKSHGKLATVTAVRPPVRFGELSISGDQVIQFQEKPQAE
EEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEECEEEECCCCEEEECCCCCCC
EGWINGGFFVFEPEVLNYIQDESTMLERSPLETLAKAGQLMAFHHAGYWQCMDTLRDKHT
CCCCCCCEEEECHHHHHHHCCHHHHHHCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
LEELWNQNKAPWKFN
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MKTVILCGGLGTRLSEETTVKPKPMVEIAGKPILWHIMKIYEYHGFGKFLLALGYKGEVI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHEEHEECCCCCHH
KDYFLNYHARMSDLTVSLKSGIIEYSNPTAEDWEVDLVDTGALTMTGGRLLRLKDQLSKE
HHHHHHHHCHHHHEEEEHHHCEEECCCCCCCCEEEEEEECCEEEECCCEEEEEHHHCCCC
TFMVTYGDGVANVDIKKLVSFHKSHGKLATVTAVRPPVRFGELSISGDQVIQFQEKPQAE
EEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCEECEEEECCCCEEEECCCCCCC
EGWINGGFFVFEPEVLNYIQDESTMLERSPLETLAKAGQLMAFHHAGYWQCMDTLRDKHT
CCCCCCCEEEECHHHHHHHCCHHHHHHCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHH
LEELWNQNKAPWKFN
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]