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The map label for this gene is wcaI

Identifier: 45657986

GI number: 45657986

Start: 2584188

End: 2585375

Strand: Reverse

Name: wcaI

Synonym: LIC12138

Alternate gene names: 45657986

Gene position: 2585375-2584188 (Counterclockwise)

Preceding gene: 45657987

Following gene: 45657985

Centisome position: 60.45

GC content: 30.98

Gene sequence:

>1188_bases
TTGAAAATTTGTATCATCGTCGACGATTATCTTCCTAAAAGCACAAAAGTCGCTGCTAAGATGATGCATGAATTGGCGGT
GAAATTAAAAGAGAAAGGACACGAAATAACAGTTATTACACCTGGAGTCGATTTGAAACAAAATTATAAAAAAGATACTT
TAGATGGAGTTTCTATTTTACAATTTTCATCGGGCAAAATTAAAAATGTTTCAAAAATTAAACGTCTTATCAACGAGTTC
GCACTTTCCAGAAGGGCTTGGAAATGTCTAAAAAAATATTTTATTCAAAATCATCATGATCTAATCATTTACTATTCTCC
TTCTATTTTTTGGGGAAATCTAGTGTTTCGTTTAAAAAATCTATGGAAGTCCCCTTCTTATCTAATTTTAAGAGATTTTT
TTCCTCAATGGATTATTGATAATGGAATGATCCGTGAAAAGTCTCTGATAGCATATTTTTTTAAATATTATGAAAAACTA
AATTACAAAACAGCAGATCAGATAGGGATACAATCTCCTGCAAATATAGAATGGTTTTCTAAAAAATTTCCTGAATTTAA
GAATATTGAACTTCTTTACAATTGGGCTACCGATTCTCCCGTTTTAAATAAGAATGACTTTTATCGCAAAAGGTTAAATT
TAGAAGGAAAGATTGTTTTTTTCTATGGTGGAAATATAGGTAAAGCTCAGGATATGATGAATATCGTTCGTTTGGCTGAA
AAAATGAAAACCTATACAGATGTTATATTCTTGTTAGTTGGAGCAGGGGATGAAGTTGAATTAGTTAAAGCGTCAATACA
AGATTTACAATTGAATAATATGATTCTCTTGGATCCTGTGTCTCAAGAGGAATTTAAACTCATGTTAGCAGAGTTTGATA
TTGGGCTTTTTACTTTACACTTTGGTCATAAAACGCATAATTTTCCAGGTAAAATTTTAGGGTATATGGTTCAGGAAAAG
CCTATTCTAGGTGTTGTAAATCCCGGAAATGATCTTAAGAATATATTGGAAGATTCTCGATCAGGTTTAGTCACAATTGC
AGGTGATGTTGAAGAACTTTATAAAAATGCACTTGTATTGTTAGACGATAAGACTAGAAAACAAATGGGTAGGAATGCAA
ATTTACTTCTTAAAAGTAAATTCTCTGTTGATAATGCTGCGGAGACAGTTTTAAACATTCTTTCTTAA

Upstream 100 bases:

>100_bases
CTATAGTATGCCGAATGTTTCTGAAAAAGTCGTTCGAATCATTCATAGTTATACAGATTACGTGAATCGGGTTATCTGGA
AAAAGTATTAGGATATTATT

Downstream 100 bases:

>100_bases
TTTATGTACGTTCGAAGACATTCTAGAATTCATGAACAAATACTTTTAAGTACTCTCTTTCAATTAACCGTTGGTAGTTT
ATTAGTGTCTTGTGTTACTA

Product: colanic acid biosynthesis glycosyl-transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKICIIVDDYLPKSTKVAAKMMHELAVKLKEKGHEITVITPGVDLKQNYKKDTLDGVSILQFSSGKIKNVSKIKRLINEF
ALSRRAWKCLKKYFIQNHHDLIIYYSPSIFWGNLVFRLKNLWKSPSYLILRDFFPQWIIDNGMIREKSLIAYFFKYYEKL
NYKTADQIGIQSPANIEWFSKKFPEFKNIELLYNWATDSPVLNKNDFYRKRLNLEGKIVFFYGGNIGKAQDMMNIVRLAE
KMKTYTDVIFLLVGAGDEVELVKASIQDLQLNNMILLDPVSQEEFKLMLAEFDIGLFTLHFGHKTHNFPGKILGYMVQEK
PILGVVNPGNDLKNILEDSRSGLVTIAGDVEELYKNALVLLDDKTRKQMGRNANLLLKSKFSVDNAAETVLNILS

Sequences:

>Translated_395_residues
MKICIIVDDYLPKSTKVAAKMMHELAVKLKEKGHEITVITPGVDLKQNYKKDTLDGVSILQFSSGKIKNVSKIKRLINEF
ALSRRAWKCLKKYFIQNHHDLIIYYSPSIFWGNLVFRLKNLWKSPSYLILRDFFPQWIIDNGMIREKSLIAYFFKYYEKL
NYKTADQIGIQSPANIEWFSKKFPEFKNIELLYNWATDSPVLNKNDFYRKRLNLEGKIVFFYGGNIGKAQDMMNIVRLAE
KMKTYTDVIFLLVGAGDEVELVKASIQDLQLNNMILLDPVSQEEFKLMLAEFDIGLFTLHFGHKTHNFPGKILGYMVQEK
PILGVVNPGNDLKNILEDSRSGLVTIAGDVEELYKNALVLLDDKTRKQMGRNANLLLKSKFSVDNAAETVLNILS
>Mature_395_residues
MKICIIVDDYLPKSTKVAAKMMHELAVKLKEKGHEITVITPGVDLKQNYKKDTLDGVSILQFSSGKIKNVSKIKRLINEF
ALSRRAWKCLKKYFIQNHHDLIIYYSPSIFWGNLVFRLKNLWKSPSYLILRDFFPQWIIDNGMIREKSLIAYFFKYYEKL
NYKTADQIGIQSPANIEWFSKKFPEFKNIELLYNWATDSPVLNKNDFYRKRLNLEGKIVFFYGGNIGKAQDMMNIVRLAE
KMKTYTDVIFLLVGAGDEVELVKASIQDLQLNNMILLDPVSQEEFKLMLAEFDIGLFTLHFGHKTHNFPGKILGYMVQEK
PILGVVNPGNDLKNILEDSRSGLVTIAGDVEELYKNALVLLDDKTRKQMGRNANLLLKSKFSVDNAAETVLNILS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 45529; Mature: 45529

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKICIIVDDYLPKSTKVAAKMMHELAVKLKEKGHEITVITPGVDLKQNYKKDTLDGVSIL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCEEEE
QFSSGKIKNVSKIKRLINEFALSRRAWKCLKKYFIQNHHDLIIYYSPSIFWGNLVFRLKN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LWKSPSYLILRDFFPQWIIDNGMIREKSLIAYFFKYYEKLNYKTADQIGIQSPANIEWFS
HHCCCCEEEEHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHH
KKFPEFKNIELLYNWATDSPVLNKNDFYRKRLNLEGKIVFFYGGNIGKAQDMMNIVRLAE
HCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KMKTYTDVIFLLVGAGDEVELVKASIQDLQLNNMILLDPVSQEEFKLMLAEFDIGLFTLH
HHHHHHHEEEEEECCCCCHHHHHHHHHHEEECCEEEECCCCHHHHHEEHHHCCCEEEEEE
FGHKTHNFPGKILGYMVQEKPILGVVNPGNDLKNILEDSRSGLVTIAGDVEELYKNALVL
ECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCHHHHHCCCEEE
LDDKTRKQMGRNANLLLKSKFSVDNAAETVLNILS
EECHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHC
>Mature Secondary Structure
MKICIIVDDYLPKSTKVAAKMMHELAVKLKEKGHEITVITPGVDLKQNYKKDTLDGVSIL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCEEEE
QFSSGKIKNVSKIKRLINEFALSRRAWKCLKKYFIQNHHDLIIYYSPSIFWGNLVFRLKN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
LWKSPSYLILRDFFPQWIIDNGMIREKSLIAYFFKYYEKLNYKTADQIGIQSPANIEWFS
HHCCCCEEEEHHHCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHH
KKFPEFKNIELLYNWATDSPVLNKNDFYRKRLNLEGKIVFFYGGNIGKAQDMMNIVRLAE
HCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KMKTYTDVIFLLVGAGDEVELVKASIQDLQLNNMILLDPVSQEEFKLMLAEFDIGLFTLH
HHHHHHHEEEEEECCCCCHHHHHHHHHHEEECCEEEECCCCHHHHHEEHHHCCCEEEEEE
FGHKTHNFPGKILGYMVQEKPILGVVNPGNDLKNILEDSRSGLVTIAGDVEELYKNALVL
ECCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCHHHHHCCCEEE
LDDKTRKQMGRNANLLLKSKFSVDNAAETVLNILS
EECHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400585; 10828401; 12057956 [H]