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The map label for this gene is rffE

Identifier: 45657987

GI number: 45657987

Start: 2585385

End: 2586515

Strand: Reverse

Name: rffE

Synonym: LIC12139

Alternate gene names: 45657987

Gene position: 2586515-2585385 (Counterclockwise)

Preceding gene: 45657988

Following gene: 45657986

Centisome position: 60.47

GC content: 34.84

Gene sequence:

>1131_bases
ATGAAAAAATTAAAAGTTTCCACAATTATAGGCACAAGGCCGGAAATCATCCGTCTATCTAGGGTTCTTGCAAAACTAGA
TCAATATTGTGATCATATCATGATTCATACCGGTCAAAATTATGACTATGAACTTAATGAGATCTTTTTTAATGATTTGG
AAATTAGAAAGCCAGATTATTTTTTAAATGCTGCGGGGACTTCGGGAGCGGCAACTATCGGAAACGTAATTATTAAAGTG
GATGAATTGCTTGCCCAAGTTCAACCAGACGCCGTTTTAGTTTTAGGAGATACGAACAGTTGTATGGCGGTAATCCCCGC
CAAAAGAAGAAAGATACCTATTTTTCACATGGAAGCTGGTAATCGCTGTTTCGACCAACGTGTCCCTGAGGAAATCAACA
GACGAATTGTTGATCATACCGCTGATATTAATTTAACTTATAGTAATATTGCTAGGGAATATCTTTTGAGAGAAGGGCTG
CCCTCTGATATGGTAATCAAGACTGGAAGTCCTATGTTTGAAGTTCTAAATTACTATTTGGAAGGAATTAAAAAATCAGA
TATTTTAGAGAAATTGAAAATTTCAGAAGGAAAGTATTTTGTCGTAAGTGCACATAGAGAAGAAAACATAGATTCGGATA
AAAATTTTGCCAAGTTGATAGATATTATTAATACAATAGCTGAAGTATTTAAATTTCCGGTCATTATTTCGACCCATCCT
AGAACTCAAAAAAAAATCAATGTATCTAATGCCTCTTTTAATCCGTTGGTTCAACTTTTAAAACCTTTAGGTTTTAAAGA
TTATAATAAACTTCAACTATCGGCAAAGGCAGTTCTTTCTGACAGCGGAACTATTACAGAAGAATCTTCTATACTCAATT
TTCCGGCTTTAAATATTCGAGAAGCGCATGAAAGACCAGAAGGAATGGAAGAGGCAAGTGTAATGATGGTTGGCTTGGAA
AAAGAAAGAGTATTACAAGTTCTTCAAATTTTAGAAAAACAACCTAAAGGTGAAGGGAGGCTTTTACGTAACGTTAGCGA
CTATAGTATGCCGAATGTTTCTGAAAAAGTCGTTCGAATCATTCATAGTTATACAGATTACGTGAATCGGGTTATCTGGA
AAAAGTATTAG

Upstream 100 bases:

>100_bases
TATTACTAATGTCGGTCAGGAGAAAATGGTCGTAATGCTTTGGGCCAGTGAAATTTTCGATAAATCTAAACCTGACACAT
ATTCCTGTAAAATTTGATTT

Downstream 100 bases:

>100_bases
GATATTATTTTGAAAATTTGTATCATCGTCGACGATTATCTTCCTAAAAGCACAAAAGTCGCTGCTAAGATGATGCATGA
ATTGGCGGTGAAATTAAAAG

Product: UDP-N-acetylglucosamine 2-epimerase

Products: NA

Alternate protein names: UDP-GlcNAc-2-epimerase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKKLKVSTIIGTRPEIIRLSRVLAKLDQYCDHIMIHTGQNYDYELNEIFFNDLEIRKPDYFLNAAGTSGAATIGNVIIKV
DELLAQVQPDAVLVLGDTNSCMAVIPAKRRKIPIFHMEAGNRCFDQRVPEEINRRIVDHTADINLTYSNIAREYLLREGL
PSDMVIKTGSPMFEVLNYYLEGIKKSDILEKLKISEGKYFVVSAHREENIDSDKNFAKLIDIINTIAEVFKFPVIISTHP
RTQKKINVSNASFNPLVQLLKPLGFKDYNKLQLSAKAVLSDSGTITEESSILNFPALNIREAHERPEGMEEASVMMVGLE
KERVLQVLQILEKQPKGEGRLLRNVSDYSMPNVSEKVVRIIHSYTDYVNRVIWKKY

Sequences:

>Translated_376_residues
MKKLKVSTIIGTRPEIIRLSRVLAKLDQYCDHIMIHTGQNYDYELNEIFFNDLEIRKPDYFLNAAGTSGAATIGNVIIKV
DELLAQVQPDAVLVLGDTNSCMAVIPAKRRKIPIFHMEAGNRCFDQRVPEEINRRIVDHTADINLTYSNIAREYLLREGL
PSDMVIKTGSPMFEVLNYYLEGIKKSDILEKLKISEGKYFVVSAHREENIDSDKNFAKLIDIINTIAEVFKFPVIISTHP
RTQKKINVSNASFNPLVQLLKPLGFKDYNKLQLSAKAVLSDSGTITEESSILNFPALNIREAHERPEGMEEASVMMVGLE
KERVLQVLQILEKQPKGEGRLLRNVSDYSMPNVSEKVVRIIHSYTDYVNRVIWKKY
>Mature_376_residues
MKKLKVSTIIGTRPEIIRLSRVLAKLDQYCDHIMIHTGQNYDYELNEIFFNDLEIRKPDYFLNAAGTSGAATIGNVIIKV
DELLAQVQPDAVLVLGDTNSCMAVIPAKRRKIPIFHMEAGNRCFDQRVPEEINRRIVDHTADINLTYSNIAREYLLREGL
PSDMVIKTGSPMFEVLNYYLEGIKKSDILEKLKISEGKYFVVSAHREENIDSDKNFAKLIDIINTIAEVFKFPVIISTHP
RTQKKINVSNASFNPLVQLLKPLGFKDYNKLQLSAKAVLSDSGTITEESSILNFPALNIREAHERPEGMEEASVMMVGLE
KERVLQVLQILEKQPKGEGRLLRNVSDYSMPNVSEKVVRIIHSYTDYVNRVIWKKY

Specific function: Catalyzes the reversible epimerization at C-2 of UDP-N- acetylglucosamine (UDP-GlcNAc) to produce UDP-N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc residues [H]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=342, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: =5.1.3.14 [H]

Molecular weight: Translated: 42843; Mature: 42843

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKVSTIIGTRPEIIRLSRVLAKLDQYCDHIMIHTGQNYDYELNEIFFNDLEIRKPDY
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEHHHHEECCCEEECCCE
FLNAAGTSGAATIGNVIIKVDELLAQVQPDAVLVLGDTNSCMAVIPAKRRKIPIFHMEAG
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEEEECCC
NRCFDQRVPEEINRRIVDHTADINLTYSNIAREYLLREGLPSDMVIKTGSPMFEVLNYYL
CCHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHH
EGIKKSDILEKLKISEGKYFVVSAHREENIDSDKNFAKLIDIINTIAEVFKFPVIISTHP
HCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCC
RTQKKINVSNASFNPLVQLLKPLGFKDYNKLQLSAKAVLSDSGTITEESSILNFPALNIR
CCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCHH
EAHERPEGMEEASVMMVGLEKERVLQVLQILEKQPKGEGRLLRNVSDYSMPNVSEKVVRI
HHHCCCCCCCHHEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHH
IHSYTDYVNRVIWKKY
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLKVSTIIGTRPEIIRLSRVLAKLDQYCDHIMIHTGQNYDYELNEIFFNDLEIRKPDY
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCEEHHHHEECCCEEECCCE
FLNAAGTSGAATIGNVIIKVDELLAQVQPDAVLVLGDTNSCMAVIPAKRRKIPIFHMEAG
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCEEEECCC
NRCFDQRVPEEINRRIVDHTADINLTYSNIAREYLLREGLPSDMVIKTGSPMFEVLNYYL
CCHHHHCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHH
EGIKKSDILEKLKISEGKYFVVSAHREENIDSDKNFAKLIDIINTIAEVFKFPVIISTHP
HCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCC
RTQKKINVSNASFNPLVQLLKPLGFKDYNKLQLSAKAVLSDSGTITEESSILNFPALNIR
CCCCEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCCCCCHH
EAHERPEGMEEASVMMVGLEKERVLQVLQILEKQPKGEGRLLRNVSDYSMPNVSEKVVRI
HHHCCCCCCCHHEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHHHHHHH
IHSYTDYVNRVIWKKY
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]