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The map label for this gene is dnaB

Identifier: 45657957

GI number: 45657957

Start: 2551407

End: 2552735

Strand: Reverse

Name: dnaB

Synonym: LIC12109

Alternate gene names: 45657957

Gene position: 2552735-2551407 (Counterclockwise)

Preceding gene: 45657958

Following gene: 45657956

Centisome position: 59.68

GC content: 41.01

Gene sequence:

>1329_bases
ATGCAGTCCGACTCCTTATATGAACCGGAATCCGAAAAAGCCTTTTTAGGATTCTTGCTTCTTAAAGGAGCAGATAATCT
AATCGACATTCCGGTTGCTCCGGAAGACTTTTATGTAGATCTGCATAGAAGAGTTTATCGAGCAATCGGAGATCTTGTAG
ACAAACGGATTACTATTGATCCGGTTTCTGTTTTAAACTTTCTCAAAGAAAACTCTCTTCTCAAAGACGAAGAAAAAGAA
TTTAATTATATCTATTCTCTTTACCGAGACACGGTAGTTACTCAACCACTTGCTTACTACGCGGTTAGGATCAAACGTTT
TTCCGAAAGAAGAATGTATTCCAAGATTCTTCAAGAATCTTTGGAACTGATCCGCAAAGAACCTGGAGACAACGAATCCG
TTTTTAATACGGTTGAAAAAAATCTTACCGAAATTTCCAGAAACATAGACGCTAAGGGTCTTCTTCCGGTCTCCTCTGAT
AAGGCCGCTCTTTCCGATTACATCATGGAAATCATGAAAAATCGGGGACAGATCACCGGTCTTCGCACCAATTTTACTAA
GTTAGACGAAGCCACTTCTGGTCTGAAAGAACACGAACTGATGATTTTGGCGGCTCGTCCCGGTAACGGTAAAACTACAT
TTGCTCTGAACATAGCGTCTAACGTGGCTTTGGTGTACAATCAACCGGTTGTTATATTTTCTTTAGAGATGAGTCGGATT
GAACTTCTCCTCAAGATGGTTTGTGCCGATTCCCAAGTAGAATCGATGAAACTGAAAAAATCGGAACTTACCAGAGCAGA
CGCTCCTAAACTTTTAGAATCTATCGTTCGTGTTACTGCTGCTCCGATCTACATAGACGATTCTGGTGGTTTGACGATAG
ACGATTTTAAAGGTAGGGTTCGTAAACTTTTGACCACCGAAAAGATTGGTCTTATCGTTGTGGACTATCTACAACTTATG
AGCGATCCTAAAAATAAAGACGGTGGTCGTCAGCAAGAGGTAGCTTCCATTTCTCGTTCTCTTAAACAAATGGCAAAAGA
AGCCAGATGTCCTATCATCGCTCTTTCTCAGATGTCTCGTGCGGTGGAGCAGAGATCTAAGGATCAAAAACCTCAACTCT
CCGACTTACGGGAGTCAGGTGCAATCGAACAAGACGCGGATATCGTTTCTTTTATCTATCGAGAAGAAAAAGTAAAAGGA
GAAGACGAAATCAGTCCGGAGATGAGAGGTAAGGCGGAAATTATCATCGCTAAAAATCGTTCTGGTCCTATTGGTTCTTT
TCATCTTGCTTTTAGGCCCGAGCTTAGCAGATTTGATAATATAGATTAA

Upstream 100 bases:

>100_bases
TAGGTTCCTATAAAATCAAGATTCGTCTTGCAGAGGGAATTCAACCTACAATTACTCTACACGTTAAAAAAGAAGAAGAG
TAATTTTTATTTAAGGGACC

Downstream 100 bases:

>100_bases
TTTTATAAGTTTTTGATAGGAAACAGAAATCATTGAAACATTGGATTCAGGAAAATTATAAAAATCGTTCTTGGGCTGGA
GAATTAAACGAATCTCAAGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MQSDSLYEPESEKAFLGFLLLKGADNLIDIPVAPEDFYVDLHRRVYRAIGDLVDKRITIDPVSVLNFLKENSLLKDEEKE
FNYIYSLYRDTVVTQPLAYYAVRIKRFSERRMYSKILQESLELIRKEPGDNESVFNTVEKNLTEISRNIDAKGLLPVSSD
KAALSDYIMEIMKNRGQITGLRTNFTKLDEATSGLKEHELMILAARPGNGKTTFALNIASNVALVYNQPVVIFSLEMSRI
ELLLKMVCADSQVESMKLKKSELTRADAPKLLESIVRVTAAPIYIDDSGGLTIDDFKGRVRKLLTTEKIGLIVVDYLQLM
SDPKNKDGGRQQEVASISRSLKQMAKEARCPIIALSQMSRAVEQRSKDQKPQLSDLRESGAIEQDADIVSFIYREEKVKG
EDEISPEMRGKAEIIIAKNRSGPIGSFHLAFRPELSRFDNID

Sequences:

>Translated_442_residues
MQSDSLYEPESEKAFLGFLLLKGADNLIDIPVAPEDFYVDLHRRVYRAIGDLVDKRITIDPVSVLNFLKENSLLKDEEKE
FNYIYSLYRDTVVTQPLAYYAVRIKRFSERRMYSKILQESLELIRKEPGDNESVFNTVEKNLTEISRNIDAKGLLPVSSD
KAALSDYIMEIMKNRGQITGLRTNFTKLDEATSGLKEHELMILAARPGNGKTTFALNIASNVALVYNQPVVIFSLEMSRI
ELLLKMVCADSQVESMKLKKSELTRADAPKLLESIVRVTAAPIYIDDSGGLTIDDFKGRVRKLLTTEKIGLIVVDYLQLM
SDPKNKDGGRQQEVASISRSLKQMAKEARCPIIALSQMSRAVEQRSKDQKPQLSDLRESGAIEQDADIVSFIYREEKVKG
EDEISPEMRGKAEIIIAKNRSGPIGSFHLAFRPELSRFDNID
>Mature_442_residues
MQSDSLYEPESEKAFLGFLLLKGADNLIDIPVAPEDFYVDLHRRVYRAIGDLVDKRITIDPVSVLNFLKENSLLKDEEKE
FNYIYSLYRDTVVTQPLAYYAVRIKRFSERRMYSKILQESLELIRKEPGDNESVFNTVEKNLTEISRNIDAKGLLPVSSD
KAALSDYIMEIMKNRGQITGLRTNFTKLDEATSGLKEHELMILAARPGNGKTTFALNIASNVALVYNQPVVIFSLEMSRI
ELLLKMVCADSQVESMKLKKSELTRADAPKLLESIVRVTAAPIYIDDSGGLTIDDFKGRVRKLLTTEKIGLIVVDYLQLM
SDPKNKDGGRQQEVASISRSLKQMAKEARCPIIALSQMSRAVEQRSKDQKPQLSDLRESGAIEQDADIVSFIYREEKVKG
EDEISPEMRGKAEIIIAKNRSGPIGSFHLAFRPELSRFDNID

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=439, Percent_Identity=35.9908883826879, Blast_Score=234, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49956; Mature: 49956

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSDSLYEPESEKAFLGFLLLKGADNLIDIPVAPEDFYVDLHRRVYRAIGDLVDKRITID
CCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
PVSVLNFLKENSLLKDEEKEFNYIYSLYRDTVVTQPLAYYAVRIKRFSERRMYSKILQES
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIRKEPGDNESVFNTVEKNLTEISRNIDAKGLLPVSSDKAALSDYIMEIMKNRGQITG
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LRTNFTKLDEATSGLKEHELMILAARPGNGKTTFALNIASNVALVYNQPVVIFSLEMSRI
EECCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCEEEEECCCEEEEEECHHHH
ELLLKMVCADSQVESMKLKKSELTRADAPKLLESIVRVTAAPIYIDDSGGLTIDDFKGRV
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHH
RKLLTTEKIGLIVVDYLQLMSDPKNKDGGRQQEVASISRSLKQMAKEARCPIIALSQMSR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
AVEQRSKDQKPQLSDLRESGAIEQDADIVSFIYREEKVKGEDEISPEMRGKAEIIIAKNR
HHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
SGPIGSFHLAFRPELSRFDNID
CCCCCEEEEEECCCHHHCCCCC
>Mature Secondary Structure
MQSDSLYEPESEKAFLGFLLLKGADNLIDIPVAPEDFYVDLHRRVYRAIGDLVDKRITID
CCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEC
PVSVLNFLKENSLLKDEEKEFNYIYSLYRDTVVTQPLAYYAVRIKRFSERRMYSKILQES
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LELIRKEPGDNESVFNTVEKNLTEISRNIDAKGLLPVSSDKAALSDYIMEIMKNRGQITG
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LRTNFTKLDEATSGLKEHELMILAARPGNGKTTFALNIASNVALVYNQPVVIFSLEMSRI
EECCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEECCCEEEEECCCEEEEEECHHHH
ELLLKMVCADSQVESMKLKKSELTRADAPKLLESIVRVTAAPIYIDDSGGLTIDDFKGRV
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCEEEHHHHHHH
RKLLTTEKIGLIVVDYLQLMSDPKNKDGGRQQEVASISRSLKQMAKEARCPIIALSQMSR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
AVEQRSKDQKPQLSDLRESGAIEQDADIVSFIYREEKVKGEDEISPEMRGKAEIIIAKNR
HHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
SGPIGSFHLAFRPELSRFDNID
CCCCCEEEEEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]