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The map label for this gene is insF1 [C]

Identifier: 45657905

GI number: 45657905

Start: 2489416

End: 2490264

Strand: Reverse

Name: insF1 [C]

Synonym: LIC12056

Alternate gene names: 45657905

Gene position: 2490264-2489416 (Counterclockwise)

Preceding gene: 45657906

Following gene: 45657900

Centisome position: 58.22

GC content: 36.98

Gene sequence:

>849_bases
ATGTTCATGGAAACTCATCGATTTGAGTATTCGATTCAAAGCATGGCTAACGTTTTAGGAGTGTCTCGTTCAGGATTCTA
TCAATTTCTAAAACGGAGTAAAAACGAATTAGAAAAATATAATCCTGAACTTGTCGAGTTCATTCGGGAAACGTGGCTAA
CAAGCCGTAAGAATTACGGCTTAGTTCGGTTGCTTCGGGAAGTGAAGAAAGTGTATTCAATTTACGGAGCAAGAACGGTT
CGAAAAGTGATGAAACTTTGTGAAATTCAAGGAAAACAGGAGAAACGTTTTCGGATTGCCACAACAGATTCTAATCATGG
GAATCGGGTTGCTCCGGACTTAGTTCAACGGAATTTCAAACCGAATCAGAAGAATCGGATCTGGGTTTCAGATATTACTT
TTTTAAGATCCTCTTTCGGGTGGATTTATCTTTGTGTAATACTGGATCTTTATTCTCGAAAAGTAGTGGGTTGGTCGATT
TCAAATTCTAATGATTCTAAGTTAGTGTGCACCGCTCTTTCCAAAGCAATCGAATGTAGAAATCCTCCTAAGGGTTTAGT
TTTTCATTCGGATCGAGGATCCAATTATTGTTCGTACGAAACTCGAAGGTATCTGTTAAATAATAAACTCAGAAGGAGTA
ATAGTAGAAAGGGAAATTGTTGGGACAACGCAGTTGCTGAGTCCTTCTTTGGTTCTCTGAAAAGAGAAATGGAATATAAT
TACTTTTATAAAATTCAAGAAGCCGAAGAATTACTTTTTGATCATATAGAGGTTTATTATAATAGACACAGATCTCATTC
GTCATTAGACTTTGTGAGTCCTGTGCAATTTGAAGTAAATGCTGCGTAA

Upstream 100 bases:

>100_bases
AGCTGAAAAAGCTTCGGAAGGAAAATCTGAAACTGAAAGAGGAAGTTACTATCCTAAAAAAGTTTGCAGCCATGCTTTCC
CGCGAACAAAATCCCGATTG

Downstream 100 bases:

>100_bases
AAATGTGTCCAATAATTCGTCACTAGGTTAGAAATTAAAGTATCTGAGTACTTAGAGCCTTTATAAACTGAGCTTATCTC
GATAACAAGAAATGATAAAA

Product: IS1500 transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLREVKKVYSIYGARTV
RKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPDLVQRNFKPNQKNRIWVSDITFLRSSFGWIYLCVILDLYSRKVVGWSI
SNSNDSKLVCTALSKAIECRNPPKGLVFHSDRGSNYCSYETRRYLLNNKLRRSNSRKGNCWDNAVAESFFGSLKREMEYN
YFYKIQEAEELLFDHIEVYYNRHRSHSSLDFVSPVQFEVNAA

Sequences:

>Translated_282_residues
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLREVKKVYSIYGARTV
RKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPDLVQRNFKPNQKNRIWVSDITFLRSSFGWIYLCVILDLYSRKVVGWSI
SNSNDSKLVCTALSKAIECRNPPKGLVFHSDRGSNYCSYETRRYLLNNKLRRSNSRKGNCWDNAVAESFFGSLKREMEYN
YFYKIQEAEELLFDHIEVYYNRHRSHSSLDFVSPVQFEVNAA
>Mature_282_residues
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYGLVRLLREVKKVYSIYGARTV
RKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPDLVQRNFKPNQKNRIWVSDITFLRSSFGWIYLCVILDLYSRKVVGWSI
SNSNDSKLVCTALSKAIECRNPPKGLVFHSDRGSNYCSYETRRYLLNNKLRRSNSRKGNCWDNAVAESFFGSLKREMEYN
YFYKIQEAEELLFDHIEVYYNRHRSHSSLDFVSPVQFEVNAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=241, Percent_Identity=29.8755186721992, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1788405, Length=281, Percent_Identity=35.2313167259786, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1787263, Length=281, Percent_Identity=35.2313167259786, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786753, Length=281, Percent_Identity=35.2313167259786, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786570, Length=281, Percent_Identity=35.2313167259786, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786490, Length=281, Percent_Identity=35.2313167259786, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1789981, Length=273, Percent_Identity=30.4029304029304, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33342; Mature: 33342

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYG
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
LVRLLREVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPDLVQRNFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCC
PNQKNRIWVSDITFLRSSFGWIYLCVILDLYSRKVVGWSISNSNDSKLVCTALSKAIECR
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCC
NPPKGLVFHSDRGSNYCSYETRRYLLNNKLRRSNSRKGNCWDNAVAESFFGSLKREMEYN
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
YFYKIQEAEELLFDHIEVYYNRHRSHSSLDFVSPVQFEVNAA
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MFMETHRFEYSIQSMANVLGVSRSGFYQFLKRSKNELEKYNPELVEFIRETWLTSRKNYG
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCC
LVRLLREVKKVYSIYGARTVRKVMKLCEIQGKQEKRFRIATTDSNHGNRVAPDLVQRNFK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHHHHCCCC
PNQKNRIWVSDITFLRSSFGWIYLCVILDLYSRKVVGWSISNSNDSKLVCTALSKAIECR
CCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHCC
NPPKGLVFHSDRGSNYCSYETRRYLLNNKLRRSNSRKGNCWDNAVAESFFGSLKREMEYN
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
YFYKIQEAEELLFDHIEVYYNRHRSHSSLDFVSPVQFEVNAA
CEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]