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The map label for this gene is gshA

Identifier: 45657677

GI number: 45657677

Start: 2203295

End: 2204857

Strand: Reverse

Name: gshA

Synonym: LIC11812

Alternate gene names: 45657677

Gene position: 2204857-2203295 (Counterclockwise)

Preceding gene: 45657678

Following gene: 45657676

Centisome position: 51.55

GC content: 34.68

Gene sequence:

>1563_bases
TTGAAAACAAAAGAACTTACTCAATCAAAAATAGAAGAGGTTTCTTTAGAAATCCTTCTGAGACACGCGGTAAAAGCAAA
ACACGGTTTAGAAAAAGAAAGTATGAGAGTCAACCCAGATGGAACTCTCTCAGGAACAACTCATCCGATTCATTTAGGTT
CTAGTTTGACCAATCACTATATCAAGACCGATTTTGCAGAACCACAACTAGAATATGCGACTCACCCTCGTCCTAAAGTA
GAAGCAAATATTAGAGAATTACAAGATTTGCATATATTTACGATTCGCAAATTGGAAAATGAACTCATCTGGCCTTTTAG
TATGCCGCCTGTTTTACCCGAAGAAGAAAACGAAATTCCACTTGGACAATATGGAACTTCTCACTCCGGAAGATGGAAAA
CGATCTATCGTCATGGATTGGGCCTTCGTTATGGAAGAAGGATGCAAACTATTTCCGGGGTTCATTATAACTTTTCTTTT
TCAAAGGTTTTCCTCAGACAGTTTTTGGGAAAAGAAATTTCTAATTTTACAAAGGAAGAGATTTCTTCTTTATACTTACA
CGTAATTCGGAATTTTTTAAGAAGGGTTCATTTTTTGACTTATCTTACAGGATCTTCTCCTGTTTTTGATTTTACGTTTT
TACCAAATCCAGGAAGTTTGAAGTTCGAAAAACATAAGAATTTTACATTATATTCAACTTATGCTACCTCTCTTAGAATG
AGCGAGATCGGATATACGAGCAAGGTTCAAGATACATTAGGAATTCATTATAATTCATTGGAAGAATACGTGGATCGTAT
GTGTTACGCGGTTCATACTCCGTACCCTAAATACGTTTCTTTTTCCGAAAATAAGGACGCTCAACTCAATCCAAATTATC
TTCAGATAGAAAATGAATTTTATTCTCCAATTCGTCCGAAACAAATTCCAAAAGGAGATGAAAGACCGTTAGACGCTCTT
CTTCAAAGAGGAATTGAGTATATAGAAATTCGTTCTTTGGATATAGATCCATATTCTCCCGTAGGAGTTTGTAGATCTAA
TCTTGCGTTTACTCAATTGATTCTTTTAGATTCTTTGTTAAAAGTATCTCCTTCTATTTCCGAAGAAGAGAACTTTAGCT
TAAAAGAAAATTTGAATTCAGTGATTTGGGAAGGAAGGAATCCAGAATTAAAAATTAACGTAAACGGATCCAAGAGAAAT
TTCCAAGAAGCAGGTGCGGAATACTCGGAGTCTCTTCGCCACTACGCTAAAATTTTAGATCTTCATACTGGAAGACGTAC
GTATCAAGAAGCGATTGATTTCCAAATCAAAAAGTGGAAAAATCCGGATAAAACTCCTTCTGGAAAATTACTTTCAGAAA
TTCTAAAACGCAATATAGAATTTAGAGAAAAGGGAATTGAACTTGCTCAAGAAAATAAACGAATGTTTTCCTATTTAGAA
TATTCACCTGGAACCTTGATGAAAATGGAAAAAGAAACGATTCGTTCTTTTCAGGAAAAAGAAGAACTAGAAAAACAGGA
AATTCAAACTCAGTATCCTACTGTTAAACTATGCAATCATTGA

Upstream 100 bases:

>100_bases
GAATTGCTCCGAACTTTGCGAAACTATTTTCTGTCAAAAGAGAGAATGGAATGCTCTATGGAGCTTGCGATCCACGTGGA
GAAGGGGCCACAGGCGGACT

Downstream 100 bases:

>100_bases
AATTGAAACCGGGAGAATTTCTTTCTCCGGAAAAATTCGTTTTAGAAGGATTTGAAGATCTAGAAATTTCTACCCAAATT
GTACTTAGAGATGCTCTGAA

Product: gamma-glutamylcysteine synthetase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVNPDGTLSGTTHPIHLGSSLTNHYIKTDFAEPQLEYATHPRPKV
EANIRELQDLHIFTIRKLENELIWPFSMPPVLPEEENEIPLGQYGTSHSGRWKTIYRHGLGLRYGRRMQTISGVHYNFSF
SKVFLRQFLGKEISNFTKEEISSLYLHVIRNFLRRVHFLTYLTGSSPVFDFTFLPNPGSLKFEKHKNFTLYSTYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYVDRMCYAVHTPYPKYVSFSENKDAQLNPNYLQIENEFYSPIRPKQIPKGDERPLDAL
LQRGIEYIEIRSLDIDPYSPVGVCRSNLAFTQLILLDSLLKVSPSISEEENFSLKENLNSVIWEGRNPELKINVNGSKRN
FQEAGAEYSESLRHYAKILDLHTGRRTYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFREKGIELAQENKRMFSYLE
YSPGTLMKMEKETIRSFQEKEELEKQEIQTQYPTVKLCNH

Sequences:

>Translated_520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVNPDGTLSGTTHPIHLGSSLTNHYIKTDFAEPQLEYATHPRPKV
EANIRELQDLHIFTIRKLENELIWPFSMPPVLPEEENEIPLGQYGTSHSGRWKTIYRHGLGLRYGRRMQTISGVHYNFSF
SKVFLRQFLGKEISNFTKEEISSLYLHVIRNFLRRVHFLTYLTGSSPVFDFTFLPNPGSLKFEKHKNFTLYSTYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYVDRMCYAVHTPYPKYVSFSENKDAQLNPNYLQIENEFYSPIRPKQIPKGDERPLDAL
LQRGIEYIEIRSLDIDPYSPVGVCRSNLAFTQLILLDSLLKVSPSISEEENFSLKENLNSVIWEGRNPELKINVNGSKRN
FQEAGAEYSESLRHYAKILDLHTGRRTYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFREKGIELAQENKRMFSYLE
YSPGTLMKMEKETIRSFQEKEELEKQEIQTQYPTVKLCNH
>Mature_520_residues
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVNPDGTLSGTTHPIHLGSSLTNHYIKTDFAEPQLEYATHPRPKV
EANIRELQDLHIFTIRKLENELIWPFSMPPVLPEEENEIPLGQYGTSHSGRWKTIYRHGLGLRYGRRMQTISGVHYNFSF
SKVFLRQFLGKEISNFTKEEISSLYLHVIRNFLRRVHFLTYLTGSSPVFDFTFLPNPGSLKFEKHKNFTLYSTYATSLRM
SEIGYTSKVQDTLGIHYNSLEEYVDRMCYAVHTPYPKYVSFSENKDAQLNPNYLQIENEFYSPIRPKQIPKGDERPLDAL
LQRGIEYIEIRSLDIDPYSPVGVCRSNLAFTQLILLDSLLKVSPSISEEENFSLKENLNSVIWEGRNPELKINVNGSKRN
FQEAGAEYSESLRHYAKILDLHTGRRTYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFREKGIELAQENKRMFSYLE
YSPGTLMKMEKETIRSFQEKEELEKQEIQTQYPTVKLCNH

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=452, Percent_Identity=43.141592920354, Blast_Score=352, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_LEPIC (Q72RD4)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001763.1
- ProteinModelPortal:   Q72RD4
- SMR:   Q72RD4
- GeneID:   2772022
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11812
- NMPDR:   fig|267671.1.peg.1763
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   CLSK574213
- BioCyc:   LINT267671:LIC_11812-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 60592; Mature: 60592

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVNPDGTLSGTTHPIHLGSSLTNHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEECCCHHHHHH
IKTDFAEPQLEYATHPRPKVEANIRELQDLHIFTIRKLENELIWPFSMPPVLPEEENEIP
HHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC
LGQYGTSHSGRWKTIYRHGLGLRYGRRMQTISGVHYNFSFSKVFLRQFLGKEISNFTKEE
CCCCCCCCCCCCHHHHHHCCCCHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
ISSLYLHVIRNFLRRVHFLTYLTGSSPVFDFTFLPNPGSLKFEKHKNFTLYSTYATSLRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHH
SEIGYTSKVQDTLGIHYNSLEEYVDRMCYAVHTPYPKYVSFSENKDAQLNPNYLQIENEF
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEEEHHH
YSPIRPKQIPKGDERPLDALLQRGIEYIEIRSLDIDPYSPVGVCRSNLAFTQLILLDSLL
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KVSPSISEEENFSLKENLNSVIWEGRNPELKINVNGSKRNFQEAGAEYSESLRHYAKILD
HCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHH
LHTGRRTYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFREKGIELAQENKRMFSYLE
HHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YSPGTLMKMEKETIRSFQEKEELEKQEIQTQYPTVKLCNH
CCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MKTKELTQSKIEEVSLEILLRHAVKAKHGLEKESMRVNPDGTLSGTTHPIHLGSSLTNHY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEECCCHHHHHH
IKTDFAEPQLEYATHPRPKVEANIRELQDLHIFTIRKLENELIWPFSMPPVLPEEENEIP
HHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC
LGQYGTSHSGRWKTIYRHGLGLRYGRRMQTISGVHYNFSFSKVFLRQFLGKEISNFTKEE
CCCCCCCCCCCCHHHHHHCCCCHHCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
ISSLYLHVIRNFLRRVHFLTYLTGSSPVFDFTFLPNPGSLKFEKHKNFTLYSTYATSLRM
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCEEEEEEHHHHHHH
SEIGYTSKVQDTLGIHYNSLEEYVDRMCYAVHTPYPKYVSFSENKDAQLNPNYLQIENEF
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCEEEEEHHH
YSPIRPKQIPKGDERPLDALLQRGIEYIEIRSLDIDPYSPVGVCRSNLAFTQLILLDSLL
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
KVSPSISEEENFSLKENLNSVIWEGRNPELKINVNGSKRNFQEAGAEYSESLRHYAKILD
HCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHCHHHHHHHHHHHHHHH
LHTGRRTYQEAIDFQIKKWKNPDKTPSGKLLSEILKRNIEFREKGIELAQENKRMFSYLE
HHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
YSPGTLMKMEKETIRSFQEKEELEKQEIQTQYPTVKLCNH
CCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA