Click here to switch to the map view.

The map label for this gene is bioF

Identifier: 45657646

GI number: 45657646

Start: 2171924

End: 2173054

Strand: Reverse

Name: bioF

Synonym: LIC11780

Alternate gene names: 45657646

Gene position: 2173054-2171924 (Counterclockwise)

Preceding gene: 161621774

Following gene: 45657645

Centisome position: 50.81

GC content: 36.25

Gene sequence:

>1131_bases
TTGAACCTGAGTCCATCTAATTTCATTCAGAAAACCTCTTCCATTTTAGAATCCTTGAAAAAGGATTTTTTATTTCGATC
TCTAGAAATTCCTTACGGAGTAGATTTATCTTCCAACAATTATCTGGGGCTTACTCACCATCCTAAACTTATTGAAAGCG
TAAAAGAAGGATTGGATCTTTATGGTGCTGGATCTGGTGCTTCTCGTTTGGTTAGTGGACATAGAAATTCTTTTGAAAGA
GCCGAGCAGTGCTGCTCCGAATGGATAGGAACTGAAACTTCTCTTTGGGTCTCAAATGGTTACTCAGCCAACGTAGGATT
GATTTCTTGTATTGCAAATGCCAAGTCTGAGGTTTTTACGGATAGGCTCAATCACGCTTCAATTTTAGATGGAATCCGTC
TTTCCGGCGCACAAAAAATATATTATAAACATCTAAACTTAAATCATTTAGAGGAACTTCTTCAAAAGTCCAATAGAAAA
GAAAAAATTATCATATCTGAAACCGTCTTTAGTATGGATGGGGATTTGGCACCGATTGAGGATCTTCTCTACCTTAAAAA
TAAATACGATGCGGTTTTAATCTTAGATGACGCACATGGAATTGGTGTATTTGGACAAAAAGGAGAAGGTAGGGTTTCTC
AAGTTTTAGGTTCAGAAAAAATCAAAGAAGTGGATTTTATCACATATACAAGTGGGAAATCTTTAGGTTTGGAAGGTGCT
TGGATAGGAACTTCTAAAATTGGAAAAGAATTTTTGATTAACAAAATGAGAACATTCATTTATTCTACAGCGCCTATGCC
TGCTATTGCACATGCGGTTCCTACTTCCATTTCAATTGTAAAGTCTATGGAAAAAGAAAGAGTAGATCTTTTACAAAAAG
CCAGTCGTTTTCGAGTTTCTATTCAGACTAAAAATTATCCAAAGACTACTTCTGAATCCCAGATTGTTCCCGTATTGTTT
TCGTCCGAAAAAGAAGTTTTAGACGCTGCAGAACTTTGTAGAAAAAACGGACTTTATGTAAAGGCGATTCGGCCTCCCAC
TGTGAATGTTCCCAGGCTTAGAATCAGTATTCATTCCGATACGACTGAATCTATATTAGAAAAATTGATTTCTATTTTAC
CGGAGTTCTAA

Upstream 100 bases:

>100_bases
TAAACGATTTTGCAAAAGAAAAATTCAAAATCACTCATGAAGAACTTGTTTCAGAAAGAAATGAAGTGAAGGAAATGCTC
TAACATTTCAAAGAGGTTTT

Downstream 100 bases:

>100_bases
TGGCAGTTTTTATAGGAGGTACTGGAACCGATGTAGGGAAAACTTTTTTTAGTTCTCTCATCTTAGGAAAATATGGAGAG
TCTTTAGGGCTTAAGTATTT

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNLSPSNFIQKTSSILESLKKDFLFRSLEIPYGVDLSSNNYLGLTHHPKLIESVKEGLDLYGAGSGASRLVSGHRNSFER
AEQCCSEWIGTETSLWVSNGYSANVGLISCIANAKSEVFTDRLNHASILDGIRLSGAQKIYYKHLNLNHLEELLQKSNRK
EKIIISETVFSMDGDLAPIEDLLYLKNKYDAVLILDDAHGIGVFGQKGEGRVSQVLGSEKIKEVDFITYTSGKSLGLEGA
WIGTSKIGKEFLINKMRTFIYSTAPMPAIAHAVPTSISIVKSMEKERVDLLQKASRFRVSIQTKNYPKTTSESQIVPVLF
SSEKEVLDAAELCRKNGLYVKAIRPPTVNVPRLRISIHSDTTESILEKLISILPEF

Sequences:

>Translated_376_residues
MNLSPSNFIQKTSSILESLKKDFLFRSLEIPYGVDLSSNNYLGLTHHPKLIESVKEGLDLYGAGSGASRLVSGHRNSFER
AEQCCSEWIGTETSLWVSNGYSANVGLISCIANAKSEVFTDRLNHASILDGIRLSGAQKIYYKHLNLNHLEELLQKSNRK
EKIIISETVFSMDGDLAPIEDLLYLKNKYDAVLILDDAHGIGVFGQKGEGRVSQVLGSEKIKEVDFITYTSGKSLGLEGA
WIGTSKIGKEFLINKMRTFIYSTAPMPAIAHAVPTSISIVKSMEKERVDLLQKASRFRVSIQTKNYPKTTSESQIVPVLF
SSEKEVLDAAELCRKNGLYVKAIRPPTVNVPRLRISIHSDTTESILEKLISILPEF
>Mature_376_residues
MNLSPSNFIQKTSSILESLKKDFLFRSLEIPYGVDLSSNNYLGLTHHPKLIESVKEGLDLYGAGSGASRLVSGHRNSFER
AEQCCSEWIGTETSLWVSNGYSANVGLISCIANAKSEVFTDRLNHASILDGIRLSGAQKIYYKHLNLNHLEELLQKSNRK
EKIIISETVFSMDGDLAPIEDLLYLKNKYDAVLILDDAHGIGVFGQKGEGRVSQVLGSEKIKEVDFITYTSGKSLGLEGA
WIGTSKIGKEFLINKMRTFIYSTAPMPAIAHAVPTSISIVKSMEKERVDLLQKASRFRVSIQTKNYPKTTSESQIVPVLF
SSEKEVLDAAELCRKNGLYVKAIRPPTVNVPRLRISIHSDTTESILEKLISILPEF

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI83977442, Length=354, Percent_Identity=30.5084745762712, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI83977440, Length=355, Percent_Identity=30.4225352112676, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI83977444, Length=355, Percent_Identity=30.4225352112676, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI7657118, Length=348, Percent_Identity=28.1609195402299, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI284448556, Length=346, Percent_Identity=27.1676300578035, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4502025, Length=352, Percent_Identity=29.8295454545455, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI40316939, Length=352, Percent_Identity=29.8295454545455, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI4758668, Length=366, Percent_Identity=29.2349726775956, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI119220554, Length=373, Percent_Identity=27.3458445040214, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI5454084, Length=235, Percent_Identity=27.2340425531915, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786993, Length=344, Percent_Identity=33.4302325581395, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1790046, Length=368, Percent_Identity=29.8913043478261, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71994529, Length=354, Percent_Identity=31.9209039548023, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17560912, Length=357, Percent_Identity=28.2913165266106, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=357, Percent_Identity=28.2913165266106, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566772, Length=366, Percent_Identity=24.5901639344262, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71982617, Length=286, Percent_Identity=22.027972027972, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71982625, Length=286, Percent_Identity=22.027972027972, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=363, Percent_Identity=29.7520661157025, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320267, Length=390, Percent_Identity=29.2307692307692, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323954, Length=278, Percent_Identity=25.5395683453237, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24662918, Length=351, Percent_Identity=32.1937321937322, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17137420, Length=348, Percent_Identity=28.735632183908, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI17136286, Length=305, Percent_Identity=28.1967213114754, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653280, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653276, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653278, Length=270, Percent_Identity=26.2962962962963, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 41744; Mature: 41744

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSPSNFIQKTSSILESLKKDFLFRSLEIPYGVDLSSNNYLGLTHHPKLIESVKEGLDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCHHHHHHHHHCCCE
YGAGSGASRLVSGHRNSFERAEQCCSEWIGTETSLWVSNGYSANVGLISCIANAKSEVFT
EECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DRLNHASILDGIRLSGAQKIYYKHLNLNHLEELLQKSNRKEKIIISETVFSMDGDLAPIE
HHCCHHHHHHCHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCHHH
DLLYLKNKYDAVLILDDAHGIGVFGQKGEGRVSQVLGSEKIKEVDFITYTSGKSLGLEGA
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHEEEEEEECCCCCCCCEE
WIGTSKIGKEFLINKMRTFIYSTAPMPAIAHAVPTSISIVKSMEKERVDLLQKASRFRVS
ECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEE
IQTKNYPKTTSESQIVPVLFSSEKEVLDAAELCRKNGLYVKAIRPPTVNVPRLRISIHSD
EEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECC
TTESILEKLISILPEF
CHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLSPSNFIQKTSSILESLKKDFLFRSLEIPYGVDLSSNNYLGLTHHPKLIESVKEGLDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCHHHHHHHHHCCCE
YGAGSGASRLVSGHRNSFERAEQCCSEWIGTETSLWVSNGYSANVGLISCIANAKSEVFT
EECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
DRLNHASILDGIRLSGAQKIYYKHLNLNHLEELLQKSNRKEKIIISETVFSMDGDLAPIE
HHCCHHHHHHCHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCHHH
DLLYLKNKYDAVLILDDAHGIGVFGQKGEGRVSQVLGSEKIKEVDFITYTSGKSLGLEGA
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHEEEEEEECCCCCCCCEE
WIGTSKIGKEFLINKMRTFIYSTAPMPAIAHAVPTSISIVKSMEKERVDLLQKASRFRVS
ECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEEEE
IQTKNYPKTTSESQIVPVLFSSEKEVLDAAELCRKNGLYVKAIRPPTVNVPRLRISIHSD
EEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECC
TTESILEKLISILPEF
CHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA