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The map label for this gene is mdh

Identifier: 161621774

GI number: 161621774

Start: 2173072

End: 2174052

Strand: Reverse

Name: mdh

Synonym: LIC11781

Alternate gene names: 161621774

Gene position: 2174052-2173072 (Counterclockwise)

Preceding gene: 45657648

Following gene: 45657646

Centisome position: 50.83

GC content: 39.96

Gene sequence:

>981_bases
ATGAGTAAGACTGTAAAAGTCGCTGTTACAGGCGCTGCGGGACAAATCGGATATTCTTTACTTTTTAGAATTGCTTCGGG
ACAAATGTTCGGCGCCGATACTGCAGTTGAAATTCAGATGCTTGAATTGGAAGCTGCAATTCCTGCGGCTAAAGGTGTCA
TTATGGAATTGGAAGACTGTGCGTTTCCTCTTCTGCAAAAGGTGACCGTCTCTTCCGATTTGGATACGGCTTTTAAAGAG
ATCAACTGGGCGTTGTTAGTTGGTTCCGTTCCTAGAAAAGCTGGAATGGAAAGAGGAGATTTACTCAAAATTAATGGTGG
GATTTTTGTAAACCAGGGAAAAGCGATTGAAAAAAATGCTGCTTCTGACGTGAGAATTCTAGTAGTTGGGAATCCTTGTA
ATACAAACTGTTTGATCGCTATGAATAACGCAAAAGGAATCTCTCAAGATCGTTGGTTTGCGATGACTAAATTGGATGAA
AACAGAGCAAAATCCCAACTTGCTTCTAAAGCAGGAGTTCCTGTAAAAGAAGTGACTCATCTTGGAATTTGGGGAAATCA
TTCCGCTACTCAGTACCCTGATTTTTACAATACAAAAATTTCTGGAAAACCGGTTACCGATGTAATCTCAGACCACGAAT
GGTTAAAAGGTGATTTTATCAAAAATGTACAACAAAGAGGCGCAGAAATTATTAAAGCAAGAGGTGCCTCTTCTGCAGCT
AGTGCAGCGAATGGGGTAGTAGACACGGTTCGAGGAATCATCACTCCTACTGCTCCTGGGGATTGTTTTTCTGCGGCGGT
TGTTTCCGATGGATCTTACGGAGCAGAAAAAGGACTTATTTTTGGATTTCCTTTAAAGTCTGACGGAAAGAAAGTAGAAA
TCATTCAAGGTATTTCATTAAACGATTTTGCAAAAGAAAAATTCAAAATCACTCATGAAGAACTTGTTTCAGAAAGAAAT
GAAGTGAAGGAAATGCTCTAA

Upstream 100 bases:

>100_bases
ACGGAGTTATGATAGAAGGTGCTCAACCGATAGAAGCTTTTATTAAGGTAATTGATCAAGAACTTAAAAATTAAAATTAG
AAATTAGGAGTCACCCAAAT

Downstream 100 bases:

>100_bases
CATTTCAAAGAGGTTTTTTGAACCTGAGTCCATCTAATTTCATTCAGAAAACCTCTTCCATTTTAGAATCCTTGAAAAAG
GATTTTTTATTTCGATCTCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSKTVKVAVTGAAGQIGYSLLFRIASGQMFGADTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDTAFKE
INWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNAASDVRILVVGNPCNTNCLIAMNNAKGISQDRWFAMTKLDE
NRAKSQLASKAGVPVKEVTHLGIWGNHSATQYPDFYNTKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
SAANGVVDTVRGIITPTAPGDCFSAAVVSDGSYGAEKGLIFGFPLKSDGKKVEIIQGISLNDFAKEKFKITHEELVSERN
EVKEML

Sequences:

>Translated_326_residues
MSKTVKVAVTGAAGQIGYSLLFRIASGQMFGADTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDTAFKE
INWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNAASDVRILVVGNPCNTNCLIAMNNAKGISQDRWFAMTKLDE
NRAKSQLASKAGVPVKEVTHLGIWGNHSATQYPDFYNTKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
SAANGVVDTVRGIITPTAPGDCFSAAVVSDGSYGAEKGLIFGFPLKSDGKKVEIIQGISLNDFAKEKFKITHEELVSERN
EVKEML
>Mature_325_residues
SKTVKVAVTGAAGQIGYSLLFRIASGQMFGADTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDTAFKEI
NWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNAASDVRILVVGNPCNTNCLIAMNNAKGISQDRWFAMTKLDEN
RAKSQLASKAGVPVKEVTHLGIWGNHSATQYPDFYNTKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAAS
AANGVVDTVRGIITPTAPGDCFSAAVVSDGSYGAEKGLIFGFPLKSDGKKVEIIQGISLNDFAKEKFKITHEELVSERNE
VKEML

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=46.5256797583082, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI89886456, Length=332, Percent_Identity=26.2048192771084, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1789632, Length=244, Percent_Identity=27.4590163934426, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=45.398773006135, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI32566798, Length=239, Percent_Identity=45.6066945606695, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=44.4444444444444, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322765, Length=302, Percent_Identity=28.476821192053, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=46.6666666666667, Blast_Score=297, Evalue=5e-81,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34918; Mature: 34787

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVKVAVTGAAGQIGYSLLFRIASGQMFGADTAVEIQMLELEAAIPAAKGVIMELEDC
CCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEEEEEHHHCCCCCCEEEEHHHH
AFPLLQKVTVSSDLDTAFKEINWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNA
HHHHHHHHHCCCHHHHHHHHCCEEEEEECCCHHCCCCCCCEEEECCCEEEECCCCCCCCC
ASDVRILVVGNPCNTNCLIAMNNAKGISQDRWFAMTKLDENRAKSQLASKAGVPVKEVTH
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHEE
LGIWGNHSATQYPDFYNTKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
EEEECCCCCCCCCCCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SAANGVVDTVRGIITPTAPGDCFSAAVVSDGSYGAEKGLIFGFPLKSDGKKVEIIQGISL
HHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
NDFAKEKFKITHEELVSERNEVKEML
CHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTVKVAVTGAAGQIGYSLLFRIASGQMFGADTAVEIQMLELEAAIPAAKGVIMELEDC
CCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEEEEEHHHCCCCCCEEEEHHHH
AFPLLQKVTVSSDLDTAFKEINWALLVGSVPRKAGMERGDLLKINGGIFVNQGKAIEKNA
HHHHHHHHHCCCHHHHHHHHCCEEEEEECCCHHCCCCCCCEEEECCCEEEECCCCCCCCC
ASDVRILVVGNPCNTNCLIAMNNAKGISQDRWFAMTKLDENRAKSQLASKAGVPVKEVTH
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHEE
LGIWGNHSATQYPDFYNTKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
EEEECCCCCCCCCCCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SAANGVVDTVRGIITPTAPGDCFSAAVVSDGSYGAEKGLIFGFPLKSDGKKVEIIQGISL
HHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
NDFAKEKFKITHEELVSERNEVKEML
CHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA