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The map label for this gene is gacS [H]

Identifier: 45657401

GI number: 45657401

Start: 1888671

End: 1890866

Strand: Reverse

Name: gacS [H]

Synonym: LIC11528

Alternate gene names: 45657401

Gene position: 1890866-1888671 (Counterclockwise)

Preceding gene: 45657402

Following gene: 45657400

Centisome position: 44.21

GC content: 35.43

Gene sequence:

>2196_bases
GTGGAACCGAACCGAACCAATTTCGAACATTCACAAGAAATTTATGAAATTCTGGTAAATCAAATTTCGGATTCCATGTT
AGTCACAGACACACAACTGGAACCCCCTGGACCTAAGATACTTTTCGTAAATCCAGCTTTTTGTAAAATGACAGGTTATA
CAAAAGAAGATTTAATCGGCAAAACTCCTAGAATGCTACAAGGCCCCTTGACAAATCGAAAAATTATGAGGGATTTAAAA
CGCTCCCTAACTCAAGGAAAGGATTTTTCCGGAGAAACGATCAACTATAAAAAAGACGGAAGCCCCTATCATGTAGAATG
GCGTATATCAGCAATTCGGGACCTTTCCGGAAATATACTATGTTTTATCTCCATACAAAGAGATATCACTGAAAAAGTAA
AAAAAGGAGAATCCGTTACGAGACACCTCCGTCTAGAAATGGGAATCACCTCGGCCACTCAGATCCTTCTCTCTACTTCG
GTAGAACTTAAAATTCTAAAATACGCAATGGAACAATTTTTAGTCTTTTTAGATTCTGAAAGACTTTATCTTTTTAAAAA
TTCTGAAAATGCAGAGTCAGCAGAACTTTATCTGGAAGTAAAAGACCCGTCTCTGGAAACCTCTGAAATTCCGGTTCTAC
AATCCATCGTTTACAATCAAGATTACTCCCGCTGGAAAAAAATTTTTTCATCCGGAGGTTATCTGCAGGGAAATTTATCT
GAGTTCTCCGAAAACGAAAAAAAATTTTTCGATCAAAGAGAAATCTGTTCGGTAACTTTAATCCCCCTATTTGTTTCAGA
CGAATGGTTTGGATTTGCAGGATTTGAGAATTTTAAAACTACAAACATAGTAAAAGAAGAAATATTTACAATTCGAACCT
TCGTAGATTTGATCAGCGTTTTTTTAGAAAGAAAAAATATATTAGAAGAATTGAAAGTCCATAGAGAAAAATTAGAAGTC
CTAGTAAGTCAAAGAACGGAAGAATTAAATCTTCAAAAAGAAATGGCGGAAAAAGCAAACAAAGCCAAATCGGAGTTTTT
GGCAAATATGAGCCACGAACTCAGAACCCCTCTCAATTCTATCATTGGGTTTTCCAGATTGATGCAATTTCCCGAAGGAA
TGGAAAGAGAAAACCGATATTTAGATCTGATCTTTCATTCTGGGGTTCATTTACTAAATATCATAAACGACATACTGGAC
CTTTCCCGTATCGAAGCGGACAAACTAGTACTCAACGAATCCGATTTCGATCTCAAGGAATTGATTTCGACTTCAGTCGA
AATGATTTTAGGAGAAGCGATTACAAAAAAACTCGAAATGACTTTCGAGTTTTTTCCTAAAAACGCCAACTTTGAAATTA
GAGCGGATCCAAAACGGATTCGACAAGTAATTCTCAATCTTCTTGGAAACGCGATCAAGTTTACAGATTCTACTGGTAAA
ATTTTAATCACTCTAAATAAACTCGAAAATAATTTCCAACTTGCAGTTCAGGATTCTGGAATCGGAATTCCTACAGAAGA
AATCTCTAAAATTTTTGAAACATTTTACCAAGTAAAAGGAGAGGATCGAGCCGCCTATGAAGGAAGCGGACTCGGCCTAC
CCATCGTTAAAAAAATCGTGGAAGCACATCATGGTTCCATCCAAGTGGAAAGTGCACTTGGCAAAGGTAGTAAGTTTGTT
GTTATTTTTCCGTCTTTCACTCGTTTAGAACATTTAAATGTTTCTAATTCTTTTTCTCAAGAAGCAGAAGAATCTCCCTA
CCCTTTCCATCTTAAATCCGTTCCCATTTTGTTATCTATTCAAGATAGAATTTATGATAGAGCGATTGAAAAATATTTCA
TACATAACTCACAAGAATTCATTTCTTTCCGTACCGTGAAAGATTCTTCCAAAATACCACAAAAAAGCCTCGATCTCCAT
AAAATATTGTTATATGATACACGTTTGCTTTTGGAAGAAACGGAAGTTGTAAAATTCTTAAGATCACTTCTAAACGAGGA
TTCCAATTTTAAACATTTGATTCTTTTGGAAACTGCGGATATTCCGCTTCCTTTTCAAGAAGATCTACTTCCTTTCTTCC
CAAACTACACGATCCAAAACCCGTTTTCTTTAGATAAATTGAAATCGATTTTAATCGAAATTGAAAAGGAGATATATCTT
GGAAATCGAACTGAAAAAGAAGAATATTCTTCGTAA

Upstream 100 bases:

>100_bases
TTAGTATCATTTTATAATTTTTTACTTTTTTAATATTTAAAACCTTCCGAAATAATAAAATTATTGATCTTTTTATAGAA
TTCAGGATTTTACATTCGCT

Downstream 100 bases:

>100_bases
AATTCCGAATGATCCTATTTTGATCATCGAAGACAAAAAAGAAAATTGTATTCTTTTAGAAAGTTTATGTGACGAACTTA
GTGTTAAACACGAGGTGGCA

Product: histidine kinase sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MEPNRTNFEHSQEIYEILVNQISDSMLVTDTQLEPPGPKILFVNPAFCKMTGYTKEDLIGKTPRMLQGPLTNRKIMRDLK
RSLTQGKDFSGETINYKKDGSPYHVEWRISAIRDLSGNILCFISIQRDITEKVKKGESVTRHLRLEMGITSATQILLSTS
VELKILKYAMEQFLVFLDSERLYLFKNSENAESAELYLEVKDPSLETSEIPVLQSIVYNQDYSRWKKIFSSGGYLQGNLS
EFSENEKKFFDQREICSVTLIPLFVSDEWFGFAGFENFKTTNIVKEEIFTIRTFVDLISVFLERKNILEELKVHREKLEV
LVSQRTEELNLQKEMAEKANKAKSEFLANMSHELRTPLNSIIGFSRLMQFPEGMERENRYLDLIFHSGVHLLNIINDILD
LSRIEADKLVLNESDFDLKELISTSVEMILGEAITKKLEMTFEFFPKNANFEIRADPKRIRQVILNLLGNAIKFTDSTGK
ILITLNKLENNFQLAVQDSGIGIPTEEISKIFETFYQVKGEDRAAYEGSGLGLPIVKKIVEAHHGSIQVESALGKGSKFV
VIFPSFTRLEHLNVSNSFSQEAEESPYPFHLKSVPILLSIQDRIYDRAIEKYFIHNSQEFISFRTVKDSSKIPQKSLDLH
KILLYDTRLLLEETEVVKFLRSLLNEDSNFKHLILLETADIPLPFQEDLLPFFPNYTIQNPFSLDKLKSILIEIEKEIYL
GNRTEKEEYSS

Sequences:

>Translated_731_residues
MEPNRTNFEHSQEIYEILVNQISDSMLVTDTQLEPPGPKILFVNPAFCKMTGYTKEDLIGKTPRMLQGPLTNRKIMRDLK
RSLTQGKDFSGETINYKKDGSPYHVEWRISAIRDLSGNILCFISIQRDITEKVKKGESVTRHLRLEMGITSATQILLSTS
VELKILKYAMEQFLVFLDSERLYLFKNSENAESAELYLEVKDPSLETSEIPVLQSIVYNQDYSRWKKIFSSGGYLQGNLS
EFSENEKKFFDQREICSVTLIPLFVSDEWFGFAGFENFKTTNIVKEEIFTIRTFVDLISVFLERKNILEELKVHREKLEV
LVSQRTEELNLQKEMAEKANKAKSEFLANMSHELRTPLNSIIGFSRLMQFPEGMERENRYLDLIFHSGVHLLNIINDILD
LSRIEADKLVLNESDFDLKELISTSVEMILGEAITKKLEMTFEFFPKNANFEIRADPKRIRQVILNLLGNAIKFTDSTGK
ILITLNKLENNFQLAVQDSGIGIPTEEISKIFETFYQVKGEDRAAYEGSGLGLPIVKKIVEAHHGSIQVESALGKGSKFV
VIFPSFTRLEHLNVSNSFSQEAEESPYPFHLKSVPILLSIQDRIYDRAIEKYFIHNSQEFISFRTVKDSSKIPQKSLDLH
KILLYDTRLLLEETEVVKFLRSLLNEDSNFKHLILLETADIPLPFQEDLLPFFPNYTIQNPFSLDKLKSILIEIEKEIYL
GNRTEKEEYSS
>Mature_731_residues
MEPNRTNFEHSQEIYEILVNQISDSMLVTDTQLEPPGPKILFVNPAFCKMTGYTKEDLIGKTPRMLQGPLTNRKIMRDLK
RSLTQGKDFSGETINYKKDGSPYHVEWRISAIRDLSGNILCFISIQRDITEKVKKGESVTRHLRLEMGITSATQILLSTS
VELKILKYAMEQFLVFLDSERLYLFKNSENAESAELYLEVKDPSLETSEIPVLQSIVYNQDYSRWKKIFSSGGYLQGNLS
EFSENEKKFFDQREICSVTLIPLFVSDEWFGFAGFENFKTTNIVKEEIFTIRTFVDLISVFLERKNILEELKVHREKLEV
LVSQRTEELNLQKEMAEKANKAKSEFLANMSHELRTPLNSIIGFSRLMQFPEGMERENRYLDLIFHSGVHLLNIINDILD
LSRIEADKLVLNESDFDLKELISTSVEMILGEAITKKLEMTFEFFPKNANFEIRADPKRIRQVILNLLGNAIKFTDSTGK
ILITLNKLENNFQLAVQDSGIGIPTEEISKIFETFYQVKGEDRAAYEGSGLGLPIVKKIVEAHHGSIQVESALGKGSKFV
VIFPSFTRLEHLNVSNSFSQEAEESPYPFHLKSVPILLSIQDRIYDRAIEKYFIHNSQEFISFRTVKDSSKIPQKSLDLH
KILLYDTRLLLEETEVVKFLRSLLNEDSNFKHLILLETADIPLPFQEDLLPFFPNYTIQNPFSLDKLKSILIEIEKEIYL
GNRTEKEEYSS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=252, Percent_Identity=40.4761904761905, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=37.9166666666667, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87081816, Length=296, Percent_Identity=35.1351351351351, Blast_Score=148, Evalue=2e-36,
Organism=Escherichia coli, GI48994928, Length=255, Percent_Identity=33.7254901960784, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1788713, Length=243, Percent_Identity=33.7448559670782, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=32, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=28.1368821292776, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786783, Length=260, Percent_Identity=24.2307692307692, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788393, Length=240, Percent_Identity=28.3333333333333, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788549, Length=255, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=25.7510729613734, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790861, Length=211, Percent_Identity=27.0142180094787, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082128, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=223, Percent_Identity=25.5605381165919, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789403, Length=209, Percent_Identity=27.7511961722488, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788279, Length=224, Percent_Identity=27.2321428571429, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=197, Percent_Identity=33.502538071066, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 84323; Mature: 84323

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPNRTNFEHSQEIYEILVNQISDSMLVTDTQLEPPGPKILFVNPAFCKMTGYTKEDLIG
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHEEECCCCHHHHCC
KTPRMLQGPLTNRKIMRDLKRSLTQGKDFSGETINYKKDGSPYHVEWRISAIRDLSGNIL
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEEHHCCCCCEE
CFISIQRDITEKVKKGESVTRHLRLEMGITSATQILLSTSVELKILKYAMEQFLVFLDSE
EEEEECHHHHHHHHCCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
RLYLFKNSENAESAELYLEVKDPSLETSEIPVLQSIVYNQDYSRWKKIFSSGGYLQGNLS
EEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCEEECCHH
EFSENEKKFFDQREICSVTLIPLFVSDEWFGFAGFENFKTTNIVKEEIFTIRTFVDLISV
HHCCHHHHHCCHHHHCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FLERKNILEELKVHREKLEVLVSQRTEELNLQKEMAEKANKAKSEFLANMSHELRTPLNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
IIGFSRLMQFPEGMERENRYLDLIFHSGVHLLNIINDILDLSRIEADKLVLNESDFDLKE
HHHHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCCHHHEEECCCCCCHHH
LISTSVEMILGEAITKKLEMTFEFFPKNANFEIRADPKRIRQVILNLLGNAIKFTDSTGK
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCCE
ILITLNKLENNFQLAVQDSGIGIPTEEISKIFETFYQVKGEDRAAYEGSGLGLPIVKKIV
EEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EAHHGSIQVESALGKGSKFVVIFPSFTRLEHLNVSNSFSQEAEESPYPFHLKSVPILLSI
HHCCCCEEEEHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHCCCCCEEECCCCEEEEH
QDRIYDRAIEKYFIHNSQEFISFRTVKDSSKIPQKSLDLHKILLYDTRLLLEETEVVKFL
HHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
RSLLNEDSNFKHLILLETADIPLPFQEDLLPFFPNYTIQNPFSLDKLKSILIEIEKEIYL
HHHHCCCCCCEEEEEEEECCCCCCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEE
GNRTEKEEYSS
CCCCCHHHCCC
>Mature Secondary Structure
MEPNRTNFEHSQEIYEILVNQISDSMLVTDTQLEPPGPKILFVNPAFCKMTGYTKEDLIG
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCHHEEECCCCHHHHCC
KTPRMLQGPLTNRKIMRDLKRSLTQGKDFSGETINYKKDGSPYHVEWRISAIRDLSGNIL
CCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEEEEEHHCCCCCEE
CFISIQRDITEKVKKGESVTRHLRLEMGITSATQILLSTSVELKILKYAMEQFLVFLDSE
EEEEECHHHHHHHHCCCHHHHHHHHEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
RLYLFKNSENAESAELYLEVKDPSLETSEIPVLQSIVYNQDYSRWKKIFSSGGYLQGNLS
EEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCEEECCHH
EFSENEKKFFDQREICSVTLIPLFVSDEWFGFAGFENFKTTNIVKEEIFTIRTFVDLISV
HHCCHHHHHCCHHHHCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FLERKNILEELKVHREKLEVLVSQRTEELNLQKEMAEKANKAKSEFLANMSHELRTPLNS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
IIGFSRLMQFPEGMERENRYLDLIFHSGVHLLNIINDILDLSRIEADKLVLNESDFDLKE
HHHHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHCCHHHEEECCCCCCHHH
LISTSVEMILGEAITKKLEMTFEFFPKNANFEIRADPKRIRQVILNLLGNAIKFTDSTGK
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHHHHCCCEEEECCCCE
ILITLNKLENNFQLAVQDSGIGIPTEEISKIFETFYQVKGEDRAAYEGSGLGLPIVKKIV
EEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
EAHHGSIQVESALGKGSKFVVIFPSFTRLEHLNVSNSFSQEAEESPYPFHLKSVPILLSI
HHCCCCEEEEHHHCCCCEEEEECCCCHHHHCCCCCCCHHHHHHCCCCCEEECCCCEEEEH
QDRIYDRAIEKYFIHNSQEFISFRTVKDSSKIPQKSLDLHKILLYDTRLLLEETEVVKFL
HHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
RSLLNEDSNFKHLILLETADIPLPFQEDLLPFFPNYTIQNPFSLDKLKSILIEIEKEIYL
HHHHCCCCCCEEEEEEEECCCCCCCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEE
GNRTEKEEYSS
CCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]