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The map label for this gene is cheB

Identifier: 45657393

GI number: 45657393

Start: 1879514

End: 1880557

Strand: Reverse

Name: cheB

Synonym: LIC11520

Alternate gene names: 45657393

Gene position: 1880557-1879514 (Counterclockwise)

Preceding gene: 45657394

Following gene: 45657392

Centisome position: 43.97

GC content: 38.31

Gene sequence:

>1044_bases
ATGATTCAAGTTTTTATCGTCGATGATTCTGCGGTAGTTCGCCAGGTTCTTACTCAAATCCTGAACAAAGATCCTGAAAT
CGAAATTATTGGTTTTGCTTCTGATCCTATTTTTGCTTCTGAAAAACTTTCCTCAGTTTGGCCGGACGTATTTATACTCG
ATATAGAAATGCCTCGTATGGACGGAATTTCTTTTTTAAAAAAAATCATGTCAGAAAAACCAACTCCAGTCATCATCTGC
TCTTCGTTAGCTGAAAAAGAATCTGAAACAGCGGTTCTTGCGATGAAACTAGGAGCGGTGGATATTATTGAAAAACCTAA
AGTGGGCTTAAAAAATTTTTTGGAAGAATCGGAAATTCTTTTTATAGATTCTGTCAGAGCCGCATCAAACGCTCGAGTCA
AAACTCATTCTTTTCAAAATGACGATTCTAAATTTTTAGAAAATCATAAACAACCTAAAACAGACTTCTCTAAGATTGAT
ACGACTGATAAACTCATTGCAATCGGTACTTCTACGGGAGGAACCCAGGCTTTAGAGTTTATTCTTACACAATTAAATAT
TCATTGTCCCGGAATCGTGATCGTACAACACATGCCCGAAAAATTTACGGAAGCGTTTGCAAATCGACTCAATCAAGTTT
GTAAGATTCAAGTAAAAGAAGCAAAAGACGGTGACCGTGTCCAGTTGGGTTCTGCGTACATCGCCCCCGGAAATAAACAC
ATGGAAATTTATCTAAGTGGAGCTCAGTTTCATATTCGAGTGTTAGACGGCCCTTTAGTAAATAGACATAGACCTTCCGT
AGATACTCTTTTTCATTCTGTGGCAAAGGCAGCGGGTAAAAACGCAAAAGGAATCATCATGACCGGAATGGGAAACGACG
GAGCCAATGGGCTTTTAAAAATGAAACAGTCCGGAGCACATACGATCGCTCAAGACGAAGCAAGTTGTGTGGTTTTTGGA
ATGCCTAAAGAAGCGATTTTAAAAGGAGCAGTAAACACAATATTACCTTTATCTAAAATTGTAGGAGAGGTTCAGTATTT
TTAG

Upstream 100 bases:

>100_bases
AACTTCTGATTTCCTCCGAAGACTTAGGGGGATTTTCTCATCGAAGAATTTTTTTTTCTCTTTGGGACGGTGAAATTTAT
GTAGAACGGCCCGAACATAC

Downstream 100 bases:

>100_bases
ACGATTTGATACATCTGCGATTCTACTTACAGCCTGATAGATTCCGTAAAATTCAAGTGGGTTACTTTGAACTTGTCCTA
AAATTTTAAAAAATTTATAG

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MIQVFIVDDSAVVRQVLTQILNKDPEIEIIGFASDPIFASEKLSSVWPDVFILDIEMPRMDGISFLKKIMSEKPTPVIIC
SSLAEKESETAVLAMKLGAVDIIEKPKVGLKNFLEESEILFIDSVRAASNARVKTHSFQNDDSKFLENHKQPKTDFSKID
TTDKLIAIGTSTGGTQALEFILTQLNIHCPGIVIVQHMPEKFTEAFANRLNQVCKIQVKEAKDGDRVQLGSAYIAPGNKH
MEIYLSGAQFHIRVLDGPLVNRHRPSVDTLFHSVAKAAGKNAKGIIMTGMGNDGANGLLKMKQSGAHTIAQDEASCVVFG
MPKEAILKGAVNTILPLSKIVGEVQYF

Sequences:

>Translated_347_residues
MIQVFIVDDSAVVRQVLTQILNKDPEIEIIGFASDPIFASEKLSSVWPDVFILDIEMPRMDGISFLKKIMSEKPTPVIIC
SSLAEKESETAVLAMKLGAVDIIEKPKVGLKNFLEESEILFIDSVRAASNARVKTHSFQNDDSKFLENHKQPKTDFSKID
TTDKLIAIGTSTGGTQALEFILTQLNIHCPGIVIVQHMPEKFTEAFANRLNQVCKIQVKEAKDGDRVQLGSAYIAPGNKH
MEIYLSGAQFHIRVLDGPLVNRHRPSVDTLFHSVAKAAGKNAKGIIMTGMGNDGANGLLKMKQSGAHTIAQDEASCVVFG
MPKEAILKGAVNTILPLSKIVGEVQYF
>Mature_347_residues
MIQVFIVDDSAVVRQVLTQILNKDPEIEIIGFASDPIFASEKLSSVWPDVFILDIEMPRMDGISFLKKIMSEKPTPVIIC
SSLAEKESETAVLAMKLGAVDIIEKPKVGLKNFLEESEILFIDSVRAASNARVKTHSFQNDDSKFLENHKQPKTDFSKID
TTDKLIAIGTSTGGTQALEFILTQLNIHCPGIVIVQHMPEKFTEAFANRLNQVCKIQVKEAKDGDRVQLGSAYIAPGNKH
MEIYLSGAQFHIRVLDGPLVNRHRPSVDTLFHSVAKAAGKNAKGIIMTGMGNDGANGLLKMKQSGAHTIAQDEASCVVFG
MPKEAILKGAVNTILPLSKIVGEVQYF

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=339, Percent_Identity=48.6725663716814, Blast_Score=350, Evalue=7e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB3_LEPIC (P62643)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001479.1
- ProteinModelPortal:   P62643
- SMR:   P62643
- GeneID:   2771487
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11520
- HOGENOM:   HBG705324
- OMA:   PGGKHML
- ProtClustDB:   CLSK574408
- BioCyc:   LINT-130-01:LINT-130-01-001479-MONOMER
- BioCyc:   LINT267671:LIC_11520-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 37988; Mature: 37988

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 171-171 ACT_SITE 197-197 ACT_SITE 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQVFIVDDSAVVRQVLTQILNKDPEIEIIGFASDPIFASEKLSSVWPDVFILDIEMPRM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCC
DGISFLKKIMSEKPTPVIICSSLAEKESETAVLAMKLGAVDIIEKPKVGLKNFLEESEIL
CHHHHHHHHHCCCCCCEEEECCHHCCCCCCEEEEEECCCEEECCCCCCCHHHHHCCCCEE
FIDSVRAASNARVKTHSFQNDDSKFLENHKQPKTDFSKIDTTDKLIAIGTSTGGTQALEF
EEECCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHCCCCCCEEEEECCCCHHHHHHH
ILTQLNIHCPGIVIVQHMPEKFTEAFANRLNQVCKIQVKEAKDGDRVQLGSAYIAPGNKH
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCEEECCCCCE
MEIYLSGAQFHIRVLDGPLVNRHRPSVDTLFHSVAKAAGKNAKGIIMTGMGNDGANGLLK
EEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEE
MKQSGAHTIAQDEASCVVFGMPKEAILKGAVNTILPLSKIVGEVQYF
ECCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQVFIVDDSAVVRQVLTQILNKDPEIEIIGFASDPIFASEKLSSVWPDVFILDIEMPRM
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCC
DGISFLKKIMSEKPTPVIICSSLAEKESETAVLAMKLGAVDIIEKPKVGLKNFLEESEIL
CHHHHHHHHHCCCCCCEEEECCHHCCCCCCEEEEEECCCEEECCCCCCCHHHHHCCCCEE
FIDSVRAASNARVKTHSFQNDDSKFLENHKQPKTDFSKIDTTDKLIAIGTSTGGTQALEF
EEECCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCHHHCCCCCCEEEEECCCCHHHHHHH
ILTQLNIHCPGIVIVQHMPEKFTEAFANRLNQVCKIQVKEAKDGDRVQLGSAYIAPGNKH
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCEEECCCCCE
MEIYLSGAQFHIRVLDGPLVNRHRPSVDTLFHSVAKAAGKNAKGIIMTGMGNDGANGLLK
EEEEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEE
MKQSGAHTIAQDEASCVVFGMPKEAILKGAVNTILPLSKIVGEVQYF
ECCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA