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The map label for this gene is prrB [H]

Identifier: 45656819

GI number: 45656819

Start: 1122397

End: 1123578

Strand: Reverse

Name: prrB [H]

Synonym: LIC10931

Alternate gene names: 45656819

Gene position: 1123578-1122397 (Counterclockwise)

Preceding gene: 45656820

Following gene: 45656818

Centisome position: 26.27

GC content: 43.23

Gene sequence:

>1182_bases
GTGAGCCAAAGGAGTTTTTTAGACCAGTTGTTGGATGGGGCGAAAGTGGAATGGAAGGCAGTTGAAGAAATTTTTGATCT
AAGAAATGGATATACTCCTTCAAAATCAATTAGTGAATATTGGAAAGATGGCACCATTCCTTGGTTTAGAATGGAAGACA
TTCGGGCAAATGGACAAATTCTTAATAATGCATTACAGAAAGTAGCAAAGAGTGCGCTTAAGGGCGGAAAATTATTTCCT
GCAAACTCAATCATAGTTGCGACATCTGCCACAATAGGCGAGCATGCTCTAATAACTGTCCCATATTTATCCAACCAGAG
GTTTACTAATTTAATTTTAAAAACTGAGTATAGCGATAGATTTGAAATTAGATTCCTTTTTTATTACTGTTTTCTATTAG
ATGATTGGTGTAAAAATAACACCACGATGTCGAGTTTTGCGTCCGTGGATATGAATGGGTTTAAAAAAATCCAAATCCCC
ATTCCGCCCCTGCCCGCCCAAGAAGAAATCGTCCGCATTCTGGACGCATTTACTGAGCTTACTACCGAGCTTGCCTCTGA
GCTTTCCGCGCGGAAAAAACAGTATAATTACTATCGAGATCAACTCTTAAGCTTTGAAGAAGGAGAAGTGGAGTGGAAGA
CGTTGGGGGAGACCTGCGACGTATATACAGGCGGGGAGGCTCCGGAAAGTAGCTCAATGAGCAAAACTCCGACAGACATC
TACAAATACCCAATTTTTGGGAACGGCGCAGAGGTATATGGCTACACAGACCGCTACAGGATAGACAAAGATGCCGTTAC
CATTTCTTCGATCGGCGCGAATACGGGAACTATATATTTTAGAAAAGCCCATTTCACCCCCATCATCAGGCTGAAGGTCG
TTATTCCAAAACAAGAAGGGATTCTGCCTCGATATCTGTTTCATGCTTTGTCCTCAATTGCGATTGGAAGCAAGAGCAGC
AGCGTCCCAAATATGAACGCGGCTGATGTGAAACGCATTTCTATCCCCATCCCTCCCCTCGCAGAACAAGAACGTATCGT
CGACATCTTAGACAAATTCGATGCCCTGACTAGCTCGATCAGCGAAGGTCTGCCTCGCGAAATTGAACTACGTCAAAAGC
AATATGAGTACTATCGTGAATTATTGTTGAGCTTCCCGAAAGCAGAAGCGGGGCCGAAATGA

Upstream 100 bases:

>100_bases
TGATCGATATCCAAAAACTCAACGCAGAGCTAAAAATCACCGTCGAAAAAATAGATCAGCTCCGTGCAGACATTGATGCA
ATCGTTGCGGAGATTGAAGG

Downstream 100 bases:

>100_bases
GCATGGATATAGCAAAGCTTTTAAAAGATGCCGATCAAAAAGTCCAGTTGCTCTATGCATTTAATGGCACGGGCAAGACT
CGCCTTTCCAGGGAATTCAA

Product: type I restriction enzyme

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSQRSFLDQLLDGAKVEWKAVEEIFDLRNGYTPSKSISEYWKDGTIPWFRMEDIRANGQILNNALQKVAKSALKGGKLFP
ANSIIVATSATIGEHALITVPYLSNQRFTNLILKTEYSDRFEIRFLFYYCFLLDDWCKNNTTMSSFASVDMNGFKKIQIP
IPPLPAQEEIVRILDAFTELTTELASELSARKKQYNYYRDQLLSFEEGEVEWKTLGETCDVYTGGEAPESSSMSKTPTDI
YKYPIFGNGAEVYGYTDRYRIDKDAVTISSIGANTGTIYFRKAHFTPIIRLKVVIPKQEGILPRYLFHALSSIAIGSKSS
SVPNMNAADVKRISIPIPPLAEQERIVDILDKFDALTSSISEGLPREIELRQKQYEYYRELLLSFPKAEAGPK

Sequences:

>Translated_393_residues
MSQRSFLDQLLDGAKVEWKAVEEIFDLRNGYTPSKSISEYWKDGTIPWFRMEDIRANGQILNNALQKVAKSALKGGKLFP
ANSIIVATSATIGEHALITVPYLSNQRFTNLILKTEYSDRFEIRFLFYYCFLLDDWCKNNTTMSSFASVDMNGFKKIQIP
IPPLPAQEEIVRILDAFTELTTELASELSARKKQYNYYRDQLLSFEEGEVEWKTLGETCDVYTGGEAPESSSMSKTPTDI
YKYPIFGNGAEVYGYTDRYRIDKDAVTISSIGANTGTIYFRKAHFTPIIRLKVVIPKQEGILPRYLFHALSSIAIGSKSS
SVPNMNAADVKRISIPIPPLAEQERIVDILDKFDALTSSISEGLPREIELRQKQYEYYRELLLSFPKAEAGPK
>Mature_392_residues
SQRSFLDQLLDGAKVEWKAVEEIFDLRNGYTPSKSISEYWKDGTIPWFRMEDIRANGQILNNALQKVAKSALKGGKLFPA
NSIIVATSATIGEHALITVPYLSNQRFTNLILKTEYSDRFEIRFLFYYCFLLDDWCKNNTTMSSFASVDMNGFKKIQIPI
PPLPAQEEIVRILDAFTELTTELASELSARKKQYNYYRDQLLSFEEGEVEWKTLGETCDVYTGGEAPESSSMSKTPTDIY
KYPIFGNGAEVYGYTDRYRIDKDAVTISSIGANTGTIYFRKAHFTPIIRLKVVIPKQEGILPRYLFHALSSIAIGSKSSS
VPNMNAADVKRISIPIPPLAEQERIVDILDKFDALTSSISEGLPREIELRQKQYEYYRELLLSFPKAEAGPK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 44588; Mature: 44457

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRSFLDQLLDGAKVEWKAVEEIFDLRNGYTPSKSISEYWKDGTIPWFRMEDIRANGQI
CCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEHHHHCCCHHH
LNNALQKVAKSALKGGKLFPANSIIVATSATIGEHALITVPYLSNQRFTNLILKTEYSDR
HHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCC
FEIRFLFYYCFLLDDWCKNNTTMSSFASVDMNGFKKIQIPIPPLPAQEEIVRILDAFTEL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
TTELASELSARKKQYNYYRDQLLSFEEGEVEWKTLGETCDVYTGGEAPESSSMSKTPTDI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
YKYPIFGNGAEVYGYTDRYRIDKDAVTISSIGANTGTIYFRKAHFTPIIRLKVVIPKQEG
EECCEECCCCEEEECCCCEEECCCCEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCC
ILPRYLFHALSSIAIGSKSSSVPNMNAADVKRISIPIPPLAEQERIVDILDKFDALTSSI
CHHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
SEGLPREIELRQKQYEYYRELLLSFPKAEAGPK
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQRSFLDQLLDGAKVEWKAVEEIFDLRNGYTPSKSISEYWKDGTIPWFRMEDIRANGQI
CHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEHHHHCCCHHH
LNNALQKVAKSALKGGKLFPANSIIVATSATIGEHALITVPYLSNQRFTNLILKTEYSDR
HHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCC
FEIRFLFYYCFLLDDWCKNNTTMSSFASVDMNGFKKIQIPIPPLPAQEEIVRILDAFTEL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
TTELASELSARKKQYNYYRDQLLSFEEGEVEWKTLGETCDVYTGGEAPESSSMSKTPTDI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
YKYPIFGNGAEVYGYTDRYRIDKDAVTISSIGANTGTIYFRKAHFTPIIRLKVVIPKQEG
EECCEECCCCEEEECCCCEEECCCCEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCC
ILPRYLFHALSSIAIGSKSSSVPNMNAADVKRISIPIPPLAEQERIVDILDKFDALTSSI
CHHHHHHHHHHHHHCCCCCCCCCCCCHHCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
SEGLPREIELRQKQYEYYRELLLSFPKAEAGPK
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]