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The map label for this gene is thiM

Identifier: 45656691

GI number: 45656691

Start: 968307

End: 969104

Strand: Reverse

Name: thiM

Synonym: LIC10800

Alternate gene names: 45656691

Gene position: 969104-968307 (Counterclockwise)

Preceding gene: 45656698

Following gene: 45656690

Centisome position: 22.66

GC content: 41.6

Gene sequence:

>798_bases
GTGTCAAAAACTACAATCATAGAAAGAATCTGGCCAGCAAAAGAGATCATTGAAGATTTATCCGAACTTAGAAAACAATC
TCCTCTAACACATGTTATTACGAACATAGTAGTAACCAATTGGACAGCAAATGTGTTACTTGCAATTGGTTCGTCCCCTG
CTATGGTAATCGCAAAAGAAGAAGCGGGAGAATTTGCAAAAATTGCAAGTGGGTTGTTAATCAATATAGGCACAGTAACC
TCTAATGACGCAATAACTATGAAAATCGCGGCAGAAGCAGCCCATCAAGCAAAAATACCTTGGGTATTAGATCCAGTAGC
CGTGGGAGCACTTGGGTTTAGAACAGAATTAGCCAAAGAATTACTTAATTTTAAACCGACCGTAATTAGAGGCAACGCAT
CCGAAATATTAGCGTTAGCCGGAACAGACGGAGGGGGAAAGGGAGTTGATTCCACAGCTCTTTCTTCTGACGCATTGCCT
CTTGCTCAAATGCTTGCCGAAAAAACTGGAGCCGTAATTGCAATCAGCGGAGAAATAGATTACGTAACCAATGGAAAAGA
AACCATTTCTATTAGCGGAGGAGACCCAATCATGACCAAAGTAACCGGGGTCGGCTGCTCCTTAGGAGGTGTAATCGCAT
CTTTTTTAGGGGTACAAAAAGATCCTCTAAGAGCAACCGCAACCGCATCCGCAGTTTTTGCAATTGCAGGAACACGCTCC
GCGAAAATATCAAAAGGTTCTGGAAGTTTTGCAGTCAATTTTTTAGATCAATTGAATTTGCTTTCGACAGAAAAATGA

Upstream 100 bases:

>100_bases
TTAAGTGATTTAAAAAAAAATCACAACATTCTTTCGAACATTCACACGGCTCAAAAAGTAAAGTTCTTTACAAGATTCAT
TCAGTCTTTAAAATAAAATC

Downstream 100 bases:

>100_bases
TTATGACTAAATTTTACTTAAGAACTATATAATAGGTTGTCACTAATCTTACTACAAAGCCCCGTTTAGTGCAAAATATT
ATGCGCTTTGTAATATAAGT

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSKTTIIERIWPAKEIIEDLSELRKQSPLTHVITNIVVTNWTANVLLAIGSSPAMVIAKEEAGEFAKIASGLLINIGTVT
SNDAITMKIAAEAAHQAKIPWVLDPVAVGALGFRTELAKELLNFKPTVIRGNASEILALAGTDGGGKGVDSTALSSDALP
LAQMLAEKTGAVIAISGEIDYVTNGKETISISGGDPIMTKVTGVGCSLGGVIASFLGVQKDPLRATATASAVFAIAGTRS
AKISKGSGSFAVNFLDQLNLLSTEK

Sequences:

>Translated_265_residues
MSKTTIIERIWPAKEIIEDLSELRKQSPLTHVITNIVVTNWTANVLLAIGSSPAMVIAKEEAGEFAKIASGLLINIGTVT
SNDAITMKIAAEAAHQAKIPWVLDPVAVGALGFRTELAKELLNFKPTVIRGNASEILALAGTDGGGKGVDSTALSSDALP
LAQMLAEKTGAVIAISGEIDYVTNGKETISISGGDPIMTKVTGVGCSLGGVIASFLGVQKDPLRATATASAVFAIAGTRS
AKISKGSGSFAVNFLDQLNLLSTEK
>Mature_264_residues
SKTTIIERIWPAKEIIEDLSELRKQSPLTHVITNIVVTNWTANVLLAIGSSPAMVIAKEEAGEFAKIASGLLINIGTVTS
NDAITMKIAAEAAHQAKIPWVLDPVAVGALGFRTELAKELLNFKPTVIRGNASEILALAGTDGGGKGVDSTALSSDALPL
AQMLAEKTGAVIAISGEIDYVTNGKETISISGGDPIMTKVTGVGCSLGGVIASFLGVQKDPLRATATASAVFAIAGTRSA
KISKGSGSFAVNFLDQLNLLSTEK

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=245, Percent_Identity=52.2448979591837, Blast_Score=249, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6325042, Length=288, Percent_Identity=33.6805555555556, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LEPIC (Q72U64)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000777.1
- ProteinModelPortal:   Q72U64
- SMR:   Q72U64
- GeneID:   2772939
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10800
- NMPDR:   fig|267671.1.peg.777
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   LINT267671:LIC_10800-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 27372; Mature: 27241

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 130-130 BINDING 176-176 BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTTIIERIWPAKEIIEDLSELRKQSPLTHVITNIVVTNWTANVLLAIGSSPAMVIAKE
CCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEC
EAGEFAKIASGLLINIGTVTSNDAITMKIAAEAAHQAKIPWVLDPVAVGALGFRTELAKE
CCCCHHHHHCCEEEEEEEECCCCEEEEEEEEHHHHHCCCCEEECHHHHHHHHHHHHHHHH
LLNFKPTVIRGNASEILALAGTDGGGKGVDSTALSSDALPLAQMLAEKTGAVIAISGEID
HHCCCCEEEECCHHHEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCEEEEECCEE
YVTNGKETISISGGDPIMTKVTGVGCSLGGVIASFLGVQKDPLRATATASAVFAIAGTRS
EEECCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHEEEEEECCCC
AKISKGSGSFAVNFLDQLNLLSTEK
CEEECCCCCEEEEHHHHHHHHCCCC
>Mature Secondary Structure 
SKTTIIERIWPAKEIIEDLSELRKQSPLTHVITNIVVTNWTANVLLAIGSSPAMVIAKE
CCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEC
EAGEFAKIASGLLINIGTVTSNDAITMKIAAEAAHQAKIPWVLDPVAVGALGFRTELAKE
CCCCHHHHHCCEEEEEEEECCCCEEEEEEEEHHHHHCCCCEEECHHHHHHHHHHHHHHHH
LLNFKPTVIRGNASEILALAGTDGGGKGVDSTALSSDALPLAQMLAEKTGAVIAISGEID
HHCCCCEEEECCHHHEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHCCCEEEEECCEE
YVTNGKETISISGGDPIMTKVTGVGCSLGGVIASFLGVQKDPLRATATASAVFAIAGTRS
EEECCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHEEEEEECCCC
AKISKGSGSFAVNFLDQLNLLSTEK
CEEECCCCCEEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA