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The map label for this gene is gst

Identifier: 45656698

GI number: 45656698

Start: 977875

End: 978369

Strand: Reverse

Name: gst

Synonym: LIC10807

Alternate gene names: 45656698

Gene position: 978369-977875 (Counterclockwise)

Preceding gene: 45656699

Following gene: 45656691

Centisome position: 22.87

GC content: 37.98

Gene sequence:

>495_bases
TTGATCGAATTATATTCGGCGTCTACACCCAACGGAAGAAAAATTTCTATTATGTTAGAAGAATTAGGAATTCCTTATAC
GGTTCACCCAATCAATTTAGATAAACTGGAACAAAAACAAGAATGGTTCCTTAAAATCAACCCAAACGGAAGAATCCCTG
CAATCATAGACAAAGACAACGAGGACTTTACAGTATTTGAATCTGGAGCAATTCTGATTTATCTCGCGGAAAAAACCGGA
AAACTTTTGCCTAAAGATCCAAAAGAAAAAAGTACCGTAATTCAATGGCTTATGTTTCAAATGGGAGGAGTCGGCCCAAT
GCAAGGACAAGCCGGAGTATTTCTTAAATACGCACCCGAGAAAATTCCTTTCGCGATCAATAGATACCAAAACGAAACTA
AACGATTATACTCCGTTTTAGATAGAAGACTAAGTGATAGTAAATTCTTAGGCGGCAAGGATTTGTCAATCGCAGACATT
GCTACCTGGCCTTAG

Upstream 100 bases:

>100_bases
TGATTCAAATATTTTTTTTTAGATTCTACTTAAAAACTGCATTTTTTCTAAAACCAAAGTCTAACTTCTAAATAAACATT
CCACAAATAGGAGAAAAACA

Downstream 100 bases:

>100_bases
GTCAATATACATGATTGGGTGGAAATCAGTCTGGACGAATTTCCTAACTTAAAACGTTGGAACGATGAACTAGAAAAACG
CCCTGCCTTTATTAAGGGCA

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MIELYSASTPNGRKISIMLEELGIPYTVHPINLDKLEQKQEWFLKINPNGRIPAIIDKDNEDFTVFESGAILIYLAEKTG
KLLPKDPKEKSTVIQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAINRYQNETKRLYSVLDRRLSDSKFLGGKDLSIADI
ATWP

Sequences:

>Translated_164_residues
MIELYSASTPNGRKISIMLEELGIPYTVHPINLDKLEQKQEWFLKINPNGRIPAIIDKDNEDFTVFESGAILIYLAEKTG
KLLPKDPKEKSTVIQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAINRYQNETKRLYSVLDRRLSDSKFLGGKDLSIADI
ATWP
>Mature_164_residues
MIELYSASTPNGRKISIMLEELGIPYTVHPINLDKLEQKQEWFLKINPNGRIPAIIDKDNEDFTVFESGAILIYLAEKTG
KLLPKDPKEKSTVIQWLMFQMGGVGPMQGQAGVFLKYAPEKIPFAINRYQNETKRLYSVLDRRLSDSKFLGGKDLSIADI
ATWP

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=168, Percent_Identity=51.7857142857143, Blast_Score=175, Evalue=1e-45,
Organism=Escherichia coli, GI87082195, Length=170, Percent_Identity=44.1176470588235, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324100, Length=147, Percent_Identity=37.4149659863946, Blast_Score=108, Evalue=6e-25,
Organism=Drosophila melanogaster, GI17864596, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17864598, Length=166, Percent_Identity=30.7228915662651, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17864594, Length=166, Percent_Identity=30.7228915662651, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17864592, Length=155, Percent_Identity=31.6129032258064, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI85725204, Length=166, Percent_Identity=30.7228915662651, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17737923, Length=166, Percent_Identity=30.7228915662651, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17933730, Length=164, Percent_Identity=29.8780487804878, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549270, Length=163, Percent_Identity=30.0613496932515, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646251, Length=104, Percent_Identity=38.4615384615385, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571670, Length=143, Percent_Identity=33.5664335664336, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 18604; Mature: 18604

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELYSASTPNGRKISIMLEELGIPYTVHPINLDKLEQKQEWFLKINPNGRIPAIIDKDN
CEEEECCCCCCCCEEEEEEHHHCCCEEEECCCHHHHCCCCEEEEEECCCCCCCEEEECCC
EDFTVFESGAILIYLAEKTGKLLPKDPKEKSTVIQWLMFQMGGVGPMQGQAGVFLKYAPE
CCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
KIPFAINRYQNETKRLYSVLDRRLSDSKFLGGKDLSIADIATWP
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MIELYSASTPNGRKISIMLEELGIPYTVHPINLDKLEQKQEWFLKINPNGRIPAIIDKDN
CEEEECCCCCCCCEEEEEEHHHCCCEEEECCCHHHHCCCCEEEEEECCCCCCCEEEECCC
EDFTVFESGAILIYLAEKTGKLLPKDPKEKSTVIQWLMFQMGGVGPMQGQAGVFLKYAPE
CCEEEEECCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCC
KIPFAINRYQNETKRLYSVLDRRLSDSKFLGGKDLSIADIATWP
CCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]