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The map label for this gene is pleD [H]

Identifier: 45656576

GI number: 45656576

Start: 822285

End: 823418

Strand: Direct

Name: pleD [H]

Synonym: LIC10678

Alternate gene names: 45656576

Gene position: 822285-823418 (Clockwise)

Preceding gene: 45656575

Following gene: 45656577

Centisome position: 19.22

GC content: 39.42

Gene sequence:

>1134_bases
TTGTCTCCAACTCCAAATTACAGTAGTTATACGGTCTTAGCGGTAGACGATTCCGAAATCAATTTGAAGTTGATTGTTCA
TACCCTCCAACCTTTTGGTTTTCAGATTTTTACCGCGGAGTCCGCCGCGGATGCGCGTAACGTTCTTCTTACCAATCGAA
TCGACGTCCTTTTGTTGGACGTAAGTATGCCCGATCAAGATGGTTTTTCGTTTTGTAGAGAACTGAGAGAAATAGAAAGA
TTTAAACTCCTTCCCATTTTATTTATTACAGGAATCAACCGTGAAGTTGGTTTTGATGAGGCGATTTCTCACGGAGGCGA
CGATTTCATTCAGAAACCGTTTCAACCTAAAGAATTAATCGCAAAGATACGAGCTTTTATTCGGATTAAAATTCTTCAGG
ACGAACTTTTAGAGCAGAAGAAAAATTACGAAAGAGAATTGATTATGGCCAGAAAGGTTCAACAGGAACTTCTTCCTGAA
AAAGAACTAGAATGGAACGGAGTTAGTTTGAGTACGATCTTTCAACCTCTGATGCAGATTGGAGGGGATTATACGGACGC
TTGGATCGAAGACGATTCTCTTCATATCTTTATAGCGGATTGTTCTGGTCATGGTCCTTCTGCGGCCTTACTTGCAGCGA
TGCTTAAGATGCAGGTTTCTAGTCTTTCTCCGGATCAGAGTTTGCAAGAAAAGGTTCAGACTCTTCGCCATAATTTAGAA
AAGATTCTTCCGGACGAGTTTTCAATTACATTCTTTTATGGAATTTTACATAAGGATTTGAGTTTTGAATATTCGAACGG
TGGTCATCCTGCTCCTATGCTCTATCAAAACGGAGAGGTTTCGATTCTTCCTGGAATGGGTCCTTTGATTATTCCGATTG
AAATCAACGTTAAAGAAGAATTTAAATCTGTCCAACTTCAAAAGGATTCTTATATGCTTCTTTATACCGATGGTGCGACC
GAGATCAGCGATAAGAGTATGAATATTCTCGGAGAAGATAAGCTAAAAAAAATATTTCAAGAATCCGTTTCTATAGGCGG
GGACATTTTAGTTTCTATGATGGAATCGATTCTTGCGCATTCCGATAGAGGTACGAATGACGACGATATTGCTATGATGG
TGTTGAAGTTATGA

Upstream 100 bases:

>100_bases
CTATCACGTTTTGTTTTTGGTTTTATTTCGTTTTGTTTATCGTTTGTCTAAGATCGAAAATCTGAAAGAAAATCTACCGG
AAAGGGAAGCGTAAAAAATC

Downstream 100 bases:

>100_bases
ATCCTTCTTACAAAGGAAAGGTCAGAGACATTTATGATCTAGGAGATAAATTGATTCTTTCTTCTTCTGATCGTATTTCC
GCGTTTGACGTTGTGTTTCC

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSPTPNYSSYTVLAVDDSEINLKLIVHTLQPFGFQIFTAESAADARNVLLTNRIDVLLLDVSMPDQDGFSFCRELREIER
FKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDELLEQKKNYERELIMARKVQQELLPE
KELEWNGVSLSTIFQPLMQIGGDYTDAWIEDDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPDQSLQEKVQTLRHNLE
KILPDEFSITFFYGILHKDLSFEYSNGGHPAPMLYQNGEVSILPGMGPLIIPIEINVKEEFKSVQLQKDSYMLLYTDGAT
EISDKSMNILGEDKLKKIFQESVSIGGDILVSMMESILAHSDRGTNDDDIAMMVLKL

Sequences:

>Translated_377_residues
MSPTPNYSSYTVLAVDDSEINLKLIVHTLQPFGFQIFTAESAADARNVLLTNRIDVLLLDVSMPDQDGFSFCRELREIER
FKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDELLEQKKNYERELIMARKVQQELLPE
KELEWNGVSLSTIFQPLMQIGGDYTDAWIEDDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPDQSLQEKVQTLRHNLE
KILPDEFSITFFYGILHKDLSFEYSNGGHPAPMLYQNGEVSILPGMGPLIIPIEINVKEEFKSVQLQKDSYMLLYTDGAT
EISDKSMNILGEDKLKKIFQESVSIGGDILVSMMESILAHSDRGTNDDDIAMMVLKL
>Mature_376_residues
SPTPNYSSYTVLAVDDSEINLKLIVHTLQPFGFQIFTAESAADARNVLLTNRIDVLLLDVSMPDQDGFSFCRELREIERF
KLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDELLEQKKNYERELIMARKVQQELLPEK
ELEWNGVSLSTIFQPLMQIGGDYTDAWIEDDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPDQSLQEKVQTLRHNLEK
ILPDEFSITFFYGILHKDLSFEYSNGGHPAPMLYQNGEVSILPGMGPLIIPIEINVKEEFKSVQLQKDSYMLLYTDGATE
ISDKSMNILGEDKLKKIFQESVSIGGDILVSMMESILAHSDRGTNDDDIAMMVLKL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=35, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=33.8983050847458, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=32.4786324786325, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=23.8297872340426, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788394, Length=95, Percent_Identity=35.7894736842105, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI145693157, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=34.453781512605, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=29.6610169491525, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42522; Mature: 42391

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTPNYSSYTVLAVDDSEINLKLIVHTLQPFGFQIFTAESAADARNVLLTNRIDVLLLD
CCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCHHHEEEECCCEEEEEE
VSMPDQDGFSFCRELREIERFKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELI
ECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHH
AKIRAFIRIKILQDELLEQKKNYERELIMARKVQQELLPEKELEWNGVSLSTIFQPLMQI
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
GGDYTDAWIEDDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPDQSLQEKVQTLRHNLE
CCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KILPDEFSITFFYGILHKDLSFEYSNGGHPAPMLYQNGEVSILPGMGPLIIPIEINVKEE
HHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEECCCEEEECCCCCEEEEEEECCHHH
FKSVQLQKDSYMLLYTDGATEISDKSMNILGEDKLKKIFQESVSIGGDILVSMMESILAH
HHHHEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
SDRGTNDDDIAMMVLKL
CCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SPTPNYSSYTVLAVDDSEINLKLIVHTLQPFGFQIFTAESAADARNVLLTNRIDVLLLD
CCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEECCCCCHHHEEEECCCEEEEEE
VSMPDQDGFSFCRELREIERFKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELI
ECCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHH
AKIRAFIRIKILQDELLEQKKNYERELIMARKVQQELLPEKELEWNGVSLSTIFQPLMQI
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHH
GGDYTDAWIEDDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPDQSLQEKVQTLRHNLE
CCCCCCCEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KILPDEFSITFFYGILHKDLSFEYSNGGHPAPMLYQNGEVSILPGMGPLIIPIEINVKEE
HHCCCCCCHHHHHHHHHHCCCEEECCCCCCCCEEEECCCEEEECCCCCEEEEEEECCHHH
FKSVQLQKDSYMLLYTDGATEISDKSMNILGEDKLKKIFQESVSIGGDILVSMMESILAH
HHHHEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
SDRGTNDDDIAMMVLKL
CCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA