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The map label for this gene is yafJ

Identifier: 45656486

GI number: 45656486

Start: 716324

End: 717100

Strand: Direct

Name: yafJ

Synonym: LIC10588

Alternate gene names: 45656486

Gene position: 716324-717100 (Clockwise)

Preceding gene: 45656482

Following gene: 45656487

Centisome position: 16.75

GC content: 38.35

Gene sequence:

>777_bases
ATGTGTGAACTTCTGGGAATGAGTGCAAATGTCCCGACAGATATTTGTTTCAGTCTGACCGGACTTGTACAAAGGGGTGG
AAAAACAGGACCTCATAGAGATGGTTGGGGAATTGCATTTTATGAAGGGAAAGGTCTGAGAGTATTTCACGATCCCGAAC
CAGGAGTAGAATCTAAAATTGCAGCCTTTGTTCAGAAGCTTCCTATTAAAAGCGAAACGGTGATCAGTCATATTCGCAAA
GCAAATCATGGAAAAGTGGAATTGAAAAATACTCATCCTTTTATTCGAGAATTTTGGGGTTCTTATTGGACCTTCGCCCA
TAACGGACAATTTAAAAAGATTAAACAAGAACCTTTAGGTGATTTTCATCCGGTCGGAAGTACGGATTCAGAACATGCTT
TTTGTTGGTTGTTGGGAAAGTTAAAACGAAAATTCAAAACTTACCCTAAAAAGGAAACAGAACTATTCAAAACCATTGAA
TCTTTATTGTGGGAACTCCATCAAAAAGGAGTTTCTAATATTCTATTAAGCGATTCTAAAAATTTATACGCGTTTTGTTC
CACAAAACTTTCCTGGATCACAAGAAAATCCCCATTTGGAAAAGCCAGACTTGTAGACGCAGACTTAAGCGTGGATTTTC
GAAAAGTAACTACACCGGGAGATCGAGTCACGGTAATTGCAACGTGCCCACTTACATCCAATGAAGAATGGACTATCTGC
AAACCGGGTGATTTTTCTGTTTGGAGAAAAGGTAAAATCGTATTTTCCAATCAATAA

Upstream 100 bases:

>100_bases
ATGATTCTCTCAAATTTTTTTTATACTGAACTTAAGGTTGTATTAAGAATTTAAAATTTAATGAATCGAGATTTGGAACA
AACTCTTAAGGAAAAACAAA

Downstream 100 bases:

>100_bases
AATTGGGATCTAATCGTAAGTTAACGTGAATTCGAGGTAGGAATTCGTTTTATAAAAACCTGATTTTTCCATAAAAAATA
AATGTGGGAACTACCCGACC

Product: glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHRDGWGIAFYEGKGLRVFHDPEPGVESKIAAFVQKLPIKSETVISHIRK
ANHGKVELKNTHPFIREFWGSYWTFAHNGQFKKIKQEPLGDFHPVGSTDSEHAFCWLLGKLKRKFKTYPKKETELFKTIE
SLLWELHQKGVSNILLSDSKNLYAFCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATCPLTSNEEWTIC
KPGDFSVWRKGKIVFSNQ

Sequences:

>Translated_258_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHRDGWGIAFYEGKGLRVFHDPEPGVESKIAAFVQKLPIKSETVISHIRK
ANHGKVELKNTHPFIREFWGSYWTFAHNGQFKKIKQEPLGDFHPVGSTDSEHAFCWLLGKLKRKFKTYPKKETELFKTIE
SLLWELHQKGVSNILLSDSKNLYAFCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATCPLTSNEEWTIC
KPGDFSVWRKGKIVFSNQ
>Mature_258_residues
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHRDGWGIAFYEGKGLRVFHDPEPGVESKIAAFVQKLPIKSETVISHIRK
ANHGKVELKNTHPFIREFWGSYWTFAHNGQFKKIKQEPLGDFHPVGSTDSEHAFCWLLGKLKRKFKTYPKKETELFKTIE
SLLWELHQKGVSNILLSDSKNLYAFCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATCPLTSNEEWTIC
KPGDFSVWRKGKIVFSNQ

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=60.2362204724409, Blast_Score=320, Evalue=7e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29218; Mature: 29218

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHRDGWGIAFYEGKGLRVFHDPEPGVESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHH
AAFVQKLPIKSETVISHIRKANHGKVELKNTHPFIREFWGSYWTFAHNGQFKKIKQEPLG
HHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHEEEEECCCHHHHHHCCCC
DFHPVGSTDSEHAFCWLLGKLKRKFKTYPKKETELFKTIESLLWELHQKGVSNILLSDSK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
NLYAFCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATCPLTSNEEWTIC
CEEEEECCHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCEEEE
KPGDFSVWRKGKIVFSNQ
CCCCCCEEECCEEEEECC
>Mature Secondary Structure
MCELLGMSANVPTDICFSLTGLVQRGGKTGPHRDGWGIAFYEGKGLRVFHDPEPGVESKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEECCCCCHHHHH
AAFVQKLPIKSETVISHIRKANHGKVELKNTHPFIREFWGSYWTFAHNGQFKKIKQEPLG
HHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHEEEEECCCHHHHHHCCCC
DFHPVGSTDSEHAFCWLLGKLKRKFKTYPKKETELFKTIESLLWELHQKGVSNILLSDSK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
NLYAFCSTKLSWITRKSPFGKARLVDADLSVDFRKVTTPGDRVTVIATCPLTSNEEWTIC
CEEEEECCHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEEECCCCCCCCEEEE
KPGDFSVWRKGKIVFSNQ
CCCCCCEEECCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]