Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 45656214

GI number: 45656214

Start: 353608

End: 354000

Strand: Reverse

Name: gcvH

Synonym: LIC10310

Alternate gene names: 45656214

Gene position: 354000-353608 (Counterclockwise)

Preceding gene: 229259641

Following gene: 45656213

Centisome position: 8.28

GC content: 40.97

Gene sequence:

>393_bases
ATGGCAGAAACACAGGCTCCCACAGGTTATCTTTTCTCCGAAAAACACGAATGGGTAAAAGTAGAAGGAGACATGGCTCT
TATCGGTATTTCGGATTTTGCTCAATCCGCATTAGGTGATATTGTGTTTGTAGATCTTCCAAAAACCGGAAAGAACATTA
AACAATTTGAAACCTTCGGAACAATCGAATCGGTAAAAGCGGCTGAAGACTTATACGCTCCTATTGGTGGAGAAGTGATT
GAATCCAATTCGGCTCTTTCTAAAAATCCGGGAGACGTCAACTCTAAACCATTCGATTCCTGGATGATTAAGGTGAAAGG
TTTTTCCACTTCCGAACTGGACAAACTTTTAACTCCGGAAAAATACAAGGCCTTAGTCGCCGGATTAGAATAA

Upstream 100 bases:

>100_bases
TCCAGATCGAAATTCGAGAACAACCGAAACAAGCCATTATAACAATGAAACCTTTTATTCCAGGCAGCATTAGAAAAAAC
TAAATTAGGAAAAACAAATT

Downstream 100 bases:

>100_bases
TAGAAAAGTCTAATATAAAGAATTTAGAAGTAGGCAAACATGAACTCGACTCTACAGAACCAAACAAAAACCAATCTTGA
AAAGGTGGGAACAGATCCTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MAETQAPTGYLFSEKHEWVKVEGDMALIGISDFAQSALGDIVFVDLPKTGKNIKQFETFGTIESVKAAEDLYAPIGGEVI
ESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELDKLLTPEKYKALVAGLE

Sequences:

>Translated_130_residues
MAETQAPTGYLFSEKHEWVKVEGDMALIGISDFAQSALGDIVFVDLPKTGKNIKQFETFGTIESVKAAEDLYAPIGGEVI
ESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELDKLLTPEKYKALVAGLE
>Mature_129_residues
AETQAPTGYLFSEKHEWVKVEGDMALIGISDFAQSALGDIVFVDLPKTGKNIKQFETFGTIESVKAAEDLYAPIGGEVIE
SNSALSKNPGDVNSKPFDSWMIKVKGFSTSELDKLLTPEKYKALVAGLE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=47.5, Blast_Score=122, Evalue=6e-29,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=48.3333333333333, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=42.4, Blast_Score=105, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=46.6101694915254, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=118, Percent_Identity=41.5254237288136, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=40.3100775193798, Blast_Score=101, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=44.0677966101695, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14118; Mature: 13987

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETQAPTGYLFSEKHEWVKVEGDMALIGISDFAQSALGDIVFVDLPKTGKNIKQFETFG
CCCCCCCCCCEECCCCCEEEEECCEEEEEHHHHHHHHHCCEEEEECCCCCCCHHHHHHHC
TIESVKAAEDLYAPIGGEVIESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELDKLLTPE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCH
KYKALVAGLE
HHHHHHHCCC
>Mature Secondary Structure 
AETQAPTGYLFSEKHEWVKVEGDMALIGISDFAQSALGDIVFVDLPKTGKNIKQFETFG
CCCCCCCCCEECCCCCEEEEECCEEEEEHHHHHHHHHCCEEEEECCCCCCCHHHHHHHC
TIESVKAAEDLYAPIGGEVIESNSALSKNPGDVNSKPFDSWMIKVKGFSTSELDKLLTPE
CHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCH
KYKALVAGLE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA