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The map label for this gene is ptpS

Identifier: 45656191

GI number: 45656191

Start: 330129

End: 330515

Strand: Direct

Name: ptpS

Synonym: LIC10287

Alternate gene names: 45656191

Gene position: 330129-330515 (Clockwise)

Preceding gene: 45656189

Following gene: 45656192

Centisome position: 7.72

GC content: 37.98

Gene sequence:

>387_bases
ATGGAAGAAATCGAACTCACAAAAGAATTTCGTTTTGACGCAGCTCACCTCCTACCCAATGTACCAGACGGTCATAAATG
CAAACGACTTCATGGGCATAGTTTTCGTTTTAAACTTCATTTAAAAGGTACGATCGACCCCAAGACCGGTTGGTTGATCG
ATTATGCGGCGGTCAGTAAAATCGTAAAACCATTGATTGAAAATCATTTGGATCATTATTATCTAAACGATGTTCCGGGA
CTTGAAAATCCTACTTCCGAAAATCTTTCTATCTGGCTTTGGAATCATCTCAAACCACTTTTGCCCTTACTTTATAAGAT
TACTTTGAATGAAACTTGTACCAGCGCTTGTATTTATGAAGGCCCTAATAAAAATTCTAGTGTGTAG

Upstream 100 bases:

>100_bases
CGTCGACTTGTTTTCAAAATTGAATTTCCAAATAGTAAAAAATTTGAAGTTACAAGTGGCGCGTAGGCGTCGGGTTCAAT
GATTATACCGGACAGGAACT

Downstream 100 bases:

>100_bases
GGATGAATTTTGAATTCTTCAAGTAACGAAAAAAATAAGGATCTAAATCGAAAAAATTTTTCCTCTAAAACTGATTCATC
AAACAACAAAGCAGTTGTAC

Product: 6-pyruvoyl tetrahydrobiopterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: CPH4 synthase; Queuosine biosynthesis protein queD [H]

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MEEIELTKEFRFDAAHLLPNVPDGHKCKRLHGHSFRFKLHLKGTIDPKTGWLIDYAAVSKIVKPLIENHLDHYYLNDVPG
LENPTSENLSIWLWNHLKPLLPLLYKITLNETCTSACIYEGPNKNSSV

Sequences:

>Translated_128_residues
MEEIELTKEFRFDAAHLLPNVPDGHKCKRLHGHSFRFKLHLKGTIDPKTGWLIDYAAVSKIVKPLIENHLDHYYLNDVPG
LENPTSENLSIWLWNHLKPLLPLLYKITLNETCTSACIYEGPNKNSSV
>Mature_128_residues
MEEIELTKEFRFDAAHLLPNVPDGHKCKRLHGHSFRFKLHLKGTIDPKTGWLIDYAAVSKIVKPLIENHLDHYYLNDVPG
LENPTSENLSIWLWNHLKPLLPLLYKITLNETCTSACIYEGPNKNSSV

Specific function: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde [H]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTPS family. QueD subfamily [H]

Homologues:

Organism=Homo sapiens, GI4506331, Length=134, Percent_Identity=29.8507462686567, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1789124, Length=121, Percent_Identity=56.198347107438, Blast_Score=154, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007116
- InterPro:   IPR007115 [H]

Pfam domain/function: PF01242 PTPS [H]

EC number: 4.2.3.12

Molecular weight: Translated: 14717; Mature: 14717

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIELTKEFRFDAAHLLPNVPDGHKCKRLHGHSFRFKLHLKGTIDPKTGWLIDYAAVSK
CCCCCCHHHHCCCHHHHCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCCEEHHHHHHH
IVKPLIENHLDHYYLNDVPGLENPTSENLSIWLWNHLKPLLPLLYKITLNETCTSACIYE
HHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEC
GPNKNSSV
CCCCCCCC
>Mature Secondary Structure
MEEIELTKEFRFDAAHLLPNVPDGHKCKRLHGHSFRFKLHLKGTIDPKTGWLIDYAAVSK
CCCCCCHHHHCCCHHHHCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCCEEHHHHHHH
IVKPLIENHLDHYYLNDVPGLENPTSENLSIWLWNHLKPLLPLLYKITLNETCTSACIYE
HHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEC
GPNKNSSV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]