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The map label for this gene is trmB

Identifier: 45656036

GI number: 45656036

Start: 151273

End: 151926

Strand: Reverse

Name: trmB

Synonym: LIC10126

Alternate gene names: 45656036

Gene position: 151926-151273 (Counterclockwise)

Preceding gene: 45656040

Following gene: 45656030

Centisome position: 3.55

GC content: 34.71

Gene sequence:

>654_bases
ATGGTCCAAGATCTCGAACAAAAACTCTGGTCGATTGCAAGTGGTATTCCCTTCTCTTCTGATTATTTTTTGCAAGCTAG
CCCTATAAGGAAACTGAAAAAAGAAAATTTATTCTCAAAAGTGTTTGAGACATATTTTTTAGAATTAGGTTCCGGTTGGG
GGGAAGTGGCAATCTCTATGGCTCTTCAAAGACCTAACACCGGTTTTATACTAATGGAAAAGAAATTTGATCGAATCCGT
CATACGATACGCGAAATCGAAAAACATTCCCTCGATAATGTAAAAATTCTCTGCGTTAATTTTAATTGGTTCCTAGAGGA
AGTATTTGAAGAAAATTTATTTTCCGAAATCCTACTGAACTTTCCAGACCCTTGGCCTAAAAAAAGACACCATAAAAAAA
GAACTGTGAATTCTAAATTTCTGGAATCTCTAAAAATTCTACTCCCTGAAAAAGGTAAATTCTATTTTGCAACGGATTAC
GGTCCCTACGCAAGAAAGATAATTCGTCTTTTCAGAGATTCGAAAGCTTTCAGTCCGGAAAAAGTAGAACTTAAATCAGA
AAGAAACGAAATTCCAGTTTCTCATTTTGAAAGAAAAAAACGGGAAGAAGGAAAAAGAATTTATTATATCGACCGGGTTC
TTGTCCAAAAATAA

Upstream 100 bases:

>100_bases
ACTCAGTGGTGTGGGGAGCGAACCTCTTACGCCGTATAGTTTTATTTTCACTGTATTATATTAGACAAACAAAATGTGTA
AAATTCCCTGACGAGAAACC

Downstream 100 bases:

>100_bases
GAATTACTAAACTCAAAGAGAGTAATTTCTAAACTTGACGATAGACTTATAAAACTTTTATAAAATAAAATTGTTGAAGA
ATTCTATAATGGGATTAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MVQDLEQKLWSIASGIPFSSDYFLQASPIRKLKKENLFSKVFETYFLELGSGWGEVAISMALQRPNTGFILMEKKFDRIR
HTIREIEKHSLDNVKILCVNFNWFLEEVFEENLFSEILLNFPDPWPKKRHHKKRTVNSKFLESLKILLPEKGKFYFATDY
GPYARKIIRLFRDSKAFSPEKVELKSERNEIPVSHFERKKREEGKRIYYIDRVLVQK

Sequences:

>Translated_217_residues
MVQDLEQKLWSIASGIPFSSDYFLQASPIRKLKKENLFSKVFETYFLELGSGWGEVAISMALQRPNTGFILMEKKFDRIR
HTIREIEKHSLDNVKILCVNFNWFLEEVFEENLFSEILLNFPDPWPKKRHHKKRTVNSKFLESLKILLPEKGKFYFATDY
GPYARKIIRLFRDSKAFSPEKVELKSERNEIPVSHFERKKREEGKRIYYIDRVLVQK
>Mature_217_residues
MVQDLEQKLWSIASGIPFSSDYFLQASPIRKLKKENLFSKVFETYFLELGSGWGEVAISMALQRPNTGFILMEKKFDRIR
HTIREIEKHSLDNVKILCVNFNWFLEEVFEENLFSEILLNFPDPWPKKRHHKKRTVNSKFLESLKILLPEKGKFYFATDY
GPYARKIIRLFRDSKAFSPEKVELKSERNEIPVSHFERKKREEGKRIYYIDRVLVQK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=167, Percent_Identity=28.7425149700599, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_LEPIC (Q72W15)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000122.1
- ProteinModelPortal:   Q72W15
- SMR:   Q72W15
- GeneID:   2771316
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10126
- NMPDR:   fig|267671.1.peg.122
- HOGENOM:   HBG529957
- OMA:   NLRIVCG
- ProtClustDB:   CLSK573374
- BioCyc:   LINT-130-01:LINT-130-01-000122-MONOMER
- BioCyc:   LINT267671:LIC_10126-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25927; Mature: 25927

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: BINDING 48-48 BINDING 73-73 BINDING 100-100 BINDING 123-123 BINDING 127-127 BINDING 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQDLEQKLWSIASGIPFSSDYFLQASPIRKLKKENLFSKVFETYFLELGSGWGEVAISM
CCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALQRPNTGFILMEKKFDRIRHTIREIEKHSLDNVKILCVNFNWFLEEVFEENLFSEILLN
HHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHC
FPDPWPKKRHHKKRTVNSKFLESLKILLPEKGKFYFATDYGPYARKIIRLFRDSKAFSPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
KVELKSERNEIPVSHFERKKREEGKRIYYIDRVLVQK
HHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHCC
>Mature Secondary Structure
MVQDLEQKLWSIASGIPFSSDYFLQASPIRKLKKENLFSKVFETYFLELGSGWGEVAISM
CCCHHHHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ALQRPNTGFILMEKKFDRIRHTIREIEKHSLDNVKILCVNFNWFLEEVFEENLFSEILLN
HHCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHC
FPDPWPKKRHHKKRTVNSKFLESLKILLPEKGKFYFATDYGPYARKIIRLFRDSKAFSPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCC
KVELKSERNEIPVSHFERKKREEGKRIYYIDRVLVQK
HHHHHHCCCCCCHHHHHHHHHHCCCEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA