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The map label for this gene is prfA

Identifier: 45656013

GI number: 45656013

Start: 121705

End: 122769

Strand: Reverse

Name: prfA

Synonym: LIC10102

Alternate gene names: 45656013

Gene position: 122769-121705 (Counterclockwise)

Preceding gene: 45656016

Following gene: 45656012

Centisome position: 2.87

GC content: 38.4

Gene sequence:

>1065_bases
ATGATAGATAGACTTGAAAAAATACAAGAAAAATACCTTCGAATCAGTGAAGAGTTAAATCAGGCAAAAGATCCTTCTTC
GCTGAAAAATCTTTATAAGGAAAGATCGAGACTCACTCCTCTGTATCTCAAAGTAGAAGAATACCTCAAAATCTATAAGG
ATAGAAAAGACGCAGAAGAATTGATTCAGTCCGAAAAAGACGAAGAAATGCATTCTATGCTTAAGGAAGAAATTCGAGAA
GCGAATCTAAAATTAGAAACTCTCGAAAGAGAATTCGAAATTCTACTTTTACCTCCAGATCCTAATTCGGGAAAAAACAT
TCTCGTGGAAATCAGAGCCGGAACCGGCGGAGAAGAAGCAGGGTTATTCGTTGCGGATTTATTTAGAATGTATTCTAAAT
TTGCGGACAAACAAAAAATTAAAACCGAAATTATAGATTCTTCTCCAACTGGAATCGGTGGTCTAAAAGAAATTATTTTC
GCTTTAGAAGATGATCGTGCTTACGATCTTTTTAAGTTCGAGGGCGGGACACATCGGGTTCAAAGAATTCCAAGCACTGA
ATCAGGAGGAAGAATTCATACGAGCGCTGTGACAGTTGCAGTTCTTCCCGAAGCAGACGAAGAAGAAGTCCAAATTAATG
AAAGCGACCTTAGAATTGATGTATATCGTTCTTCCGGTGCGGGAGGTCAGCACGTAAATACTACAGACTCTGCGGTTCGG
ATTACCCATATTCCTACCGGAGTTGTAGTTGCGTGTCAGGACGAAAAATCTCAGCATAAAAATAAGGCAAAGGCTTTGAG
AATTTTAAGCGCTCGAATTTTAGAAAAACAAGCCGAAGATAAAAAGCAAGCTTCGGATGCGATCAAAAAACAAATGATTG
GAAGTGGAGATCGTTCTGAAAGAGTAAGAACTTATAATTTTCCTCAAGGTAGATGTACAGATCATAGAATCGGTTTTACG
AGCCATAATCTTTCTGCAATTATGGAAGGAGACTTAGACGAATTGATCGGAGCCTTAACAGAAGAGGATAGAGTTCGAAA
AATTTCCGAGACTCAAACACATTAG

Upstream 100 bases:

>100_bases
GGTCAATAAGGATAGAAATTTTTCAGAAAAAACTTCTGCGAAGGAAATATGGTTTTCCATAGAGTGCGTTGATTTATAAA
GGAAAGGTCGAGTTCATTCA

Downstream 100 bases:

>100_bases
AATTTTAATAAATTTACATATTAAAAAATTCCCAATGAACAACCACTGGAATCCATAAAATGTTAAAAGAAATCAACGAA
CCACACCGAACACTTTGCGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIDRLEKIQEKYLRISEELNQAKDPSSLKNLYKERSRLTPLYLKVEEYLKIYKDRKDAEELIQSEKDEEMHSMLKEEIRE
ANLKLETLEREFEILLLPPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKTEIIDSSPTGIGGLKEIIF
ALEDDRAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEVQINESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQAEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLDELIGALTEEDRVRKISETQTH

Sequences:

>Translated_354_residues
MIDRLEKIQEKYLRISEELNQAKDPSSLKNLYKERSRLTPLYLKVEEYLKIYKDRKDAEELIQSEKDEEMHSMLKEEIRE
ANLKLETLEREFEILLLPPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKTEIIDSSPTGIGGLKEIIF
ALEDDRAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEVQINESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQAEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLDELIGALTEEDRVRKISETQTH
>Mature_354_residues
MIDRLEKIQEKYLRISEELNQAKDPSSLKNLYKERSRLTPLYLKVEEYLKIYKDRKDAEELIQSEKDEEMHSMLKEEIRE
ANLKLETLEREFEILLLPPDPNSGKNILVEIRAGTGGEEAGLFVADLFRMYSKFADKQKIKTEIIDSSPTGIGGLKEIIF
ALEDDRAYDLFKFEGGTHRVQRIPSTESGGRIHTSAVTVAVLPEADEEEVQINESDLRIDVYRSSGAGGQHVNTTDSAVR
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQAEDKKQASDAIKKQMIGSGDRSERVRTYNFPQGRCTDHRIGFT
SHNLSAIMEGDLDELIGALTEEDRVRKISETQTH

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=291, Percent_Identity=44.3298969072165, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI34577120, Length=322, Percent_Identity=36.0248447204969, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI166795305, Length=194, Percent_Identity=42.2680412371134, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=48.7394957983193, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=36.5781710914454, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=35.6363636363636, Blast_Score=170, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=42.2492401215805, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19921226, Length=296, Percent_Identity=40.2027027027027, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40100; Mature: 40100

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRLEKIQEKYLRISEELNQAKDPSSLKNLYKERSRLTPLYLKVEEYLKIYKDRKDAEE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCHHHH
LIQSEKDEEMHSMLKEEIREANLKLETLEREFEILLLPPDPNSGKNILVEIRAGTGGEEA
HHHCCCHHHHHHHHHHHHHHHCCHHEEECCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFVADLFRMYSKFADKQKIKTEIIDSSPTGIGGLKEIIFALEDDRAYDLFKFEGGTHRV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHH
QRIPSTESGGRIHTSAVTVAVLPEADEEEVQINESDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQAEDKKQASDAIKKQMIGSGDRSE
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRTYNFPQGRCTDHRIGFTSHNLSAIMEGDLDELIGALTEEDRVRKISETQTH
CCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIDRLEKIQEKYLRISEELNQAKDPSSLKNLYKERSRLTPLYLKVEEYLKIYKDRKDAEE
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCHHHH
LIQSEKDEEMHSMLKEEIREANLKLETLEREFEILLLPPDPNSGKNILVEIRAGTGGEEA
HHHCCCHHHHHHHHHHHHHHHCCHHEEECCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
GLFVADLFRMYSKFADKQKIKTEIIDSSPTGIGGLKEIIFALEDDRAYDLFKFEGGTHRV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHH
QRIPSTESGGRIHTSAVTVAVLPEADEEEVQINESDLRIDVYRSSGAGGQHVNTTDSAVR
HCCCCCCCCCEEEEEEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCCCCCCCCCEEE
ITHIPTGVVVACQDEKSQHKNKAKALRILSARILEKQAEDKKQASDAIKKQMIGSGDRSE
EEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRTYNFPQGRCTDHRIGFTSHNLSAIMEGDLDELIGALTEEDRVRKISETQTH
CCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA