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The map label for this gene is kpsF

Identifier: 45655954

GI number: 45655954

Start: 49034

End: 50002

Strand: Reverse

Name: kpsF

Synonym: LIC10040

Alternate gene names: 45655954

Gene position: 50002-49034 (Counterclockwise)

Preceding gene: 45655955

Following gene: 45655953

Centisome position: 1.17

GC content: 35.91

Gene sequence:

>969_bases
TTGGATCCAATTTTTGAAAAGATAGAAAAAGCAATCGATACCGAAATAGAATCCATTCTTTACTTTAGAAAAAATTTAGA
TCCATCTATTAAACAAGCAATCGAATTGATTTTAGAATGCAAAGGAAAATTAATCGTTACTGGCGTAGGCAAATCAGGAG
ACGTAGGAAAAAAAATTTCTTCTACTTTATCTTCCACAGGAACACCTTCTGTTTTTTTACATCCCGCAGACGCAGCCCAC
GGAGACGCCGGAATCATTTCGTGTGAAGACATTATCATAGCCATCGGCAAATCAGGTGAAAGTGAGGAATTACTAAATCT
AATTCCAACAATTAAAAATATAGGTGCAAAATTAGTTTCGATGACGGCTAACGTAGATTCTAAACTTGCAAAAGAATCAG
ACGTTGTTTTGATCACGCCAGTTTTAAAAGAAGCCTGTCCTCTTGAGCTAGCTCCTACATCTAGTACCACGATTGCTTTG
ATTTTAGGAGACGCCATTGCTATGTGTTTGATGGAATTGAAAAATTTTAAAAGAGAAAATTTTGCGCTTTATCATCCAGC
GGGCAGACTTGGAAAACGTTTGAGTTTGAAAATAGATGATGTAATGAGGAAAGATAAAGATTTAGCCAAGGTATTACCGG
ATACAAAGTTAGAAAATATACTCACGGAAATTACAGTAAAAAGACAAGGGGCCACGGGAGTTACAGATTTAAACGGAACA
CTTTTAGGAATCATTACAGACTTTGATATTCGTAAAAAACTAAAAGAAGGAAAATTGGATTCCTCAATTTCGGCAGAACA
ATTGATGAACCCAAGTCCTACTATGTTTCTATCTGGATCAAATGCTTATGACGTTTTAAAACAAATGGAATCTAGGCCTA
ATCCAATTTCAGTCGCTCCGATTGTAGATAATTCCAAAAGACTGATTGGAATCGTTTCCATCCACGACCTTTTACAAAAA
GGTCTTTGA

Upstream 100 bases:

>100_bases
CAAAGAAAAAATGAAGAACAAATCGTCGTTAAATTCCCCGGAATGAATTTGGTGACATCGGTAACTCTGGATATAAAAAC
GTTAAAATTGGAGGAAACTC

Downstream 100 bases:

>100_bases
TTTATGAATCAGCGAGAATTTAAAATCGTTCTTACGATTAACGAAGACGAATTCTTTTCTCTTAAAAAACATCCCTTTTG
TGATCGAATTGAAATTCGCC

Product: polysialic acid capsule expression protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDPIFEKIEKAIDTEIESILYFRKNLDPSIKQAIELILECKGKLIVTGVGKSGDVGKKISSTLSSTGTPSVFLHPADAAH
GDAGIISCEDIIIAIGKSGESEELLNLIPTIKNIGAKLVSMTANVDSKLAKESDVVLITPVLKEACPLELAPTSSTTIAL
ILGDAIAMCLMELKNFKRENFALYHPAGRLGKRLSLKIDDVMRKDKDLAKVLPDTKLENILTEITVKRQGATGVTDLNGT
LLGIITDFDIRKKLKEGKLDSSISAEQLMNPSPTMFLSGSNAYDVLKQMESRPNPISVAPIVDNSKRLIGIVSIHDLLQK
GL

Sequences:

>Translated_322_residues
MDPIFEKIEKAIDTEIESILYFRKNLDPSIKQAIELILECKGKLIVTGVGKSGDVGKKISSTLSSTGTPSVFLHPADAAH
GDAGIISCEDIIIAIGKSGESEELLNLIPTIKNIGAKLVSMTANVDSKLAKESDVVLITPVLKEACPLELAPTSSTTIAL
ILGDAIAMCLMELKNFKRENFALYHPAGRLGKRLSLKIDDVMRKDKDLAKVLPDTKLENILTEITVKRQGATGVTDLNGT
LLGIITDFDIRKKLKEGKLDSSISAEQLMNPSPTMFLSGSNAYDVLKQMESRPNPISVAPIVDNSKRLIGIVSIHDLLQK
GL
>Mature_322_residues
MDPIFEKIEKAIDTEIESILYFRKNLDPSIKQAIELILECKGKLIVTGVGKSGDVGKKISSTLSSTGTPSVFLHPADAAH
GDAGIISCEDIIIAIGKSGESEELLNLIPTIKNIGAKLVSMTANVDSKLAKESDVVLITPVLKEACPLELAPTSSTTIAL
ILGDAIAMCLMELKNFKRENFALYHPAGRLGKRLSLKIDDVMRKDKDLAKVLPDTKLENILTEITVKRQGATGVTDLNGT
LLGIITDFDIRKKLKEGKLDSSISAEQLMNPSPTMFLSGSNAYDVLKQMESRPNPISVAPIVDNSKRLIGIVSIHDLLQK
GL

Specific function: Unknown

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=308, Percent_Identity=39.9350649350649, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082151, Length=309, Percent_Identity=37.2168284789644, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 34772; Mature: 34772

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIFEKIEKAIDTEIESILYFRKNLDPSIKQAIELILECKGKLIVTGVGKSGDVGKKIS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
STLSSTGTPSVFLHPADAAHGDAGIISCEDIIIAIGKSGESEELLNLIPTIKNIGAKLVS
HHHHCCCCCEEEEECCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MTANVDSKLAKESDVVLITPVLKEACPLELAPTSSTTIALILGDAIAMCLMELKNFKREN
HHCCHHHHHHCCCCEEEEEHHHHHCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCC
FALYHPAGRLGKRLSLKIDDVMRKDKDLAKVLPDTKLENILTEITVKRQGATGVTDLNGT
EEEECCHHHHCCEEEEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCE
LLGIITDFDIRKKLKEGKLDSSISAEQLMNPSPTMFLSGSNAYDVLKQMESRPNPISVAP
EEEEEECHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEE
IVDNSKRLIGIVSIHDLLQKGL
EECCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MDPIFEKIEKAIDTEIESILYFRKNLDPSIKQAIELILECKGKLIVTGVGKSGDVGKKIS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
STLSSTGTPSVFLHPADAAHGDAGIISCEDIIIAIGKSGESEELLNLIPTIKNIGAKLVS
HHHHCCCCCEEEEECCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
MTANVDSKLAKESDVVLITPVLKEACPLELAPTSSTTIALILGDAIAMCLMELKNFKREN
HHCCHHHHHHCCCCEEEEEHHHHHCCCCEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCC
FALYHPAGRLGKRLSLKIDDVMRKDKDLAKVLPDTKLENILTEITVKRQGATGVTDLNGT
EEEECCHHHHCCEEEEEHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCE
LLGIITDFDIRKKLKEGKLDSSISAEQLMNPSPTMFLSGSNAYDVLKQMESRPNPISVAP
EEEEEECHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCEEEEE
IVDNSKRLIGIVSIHDLLQKGL
EECCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]