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The map label for this gene is pykF [C]

Identifier: 45359168

GI number: 45359168

Start: 1552396

End: 1553739

Strand: Direct

Name: pykF [C]

Synonym: MMP1605

Alternate gene names: 45359168

Gene position: 1552396-1553739 (Clockwise)

Preceding gene: 45359166

Following gene: 45359169

Centisome position: 93.45

GC content: 30.88

Gene sequence:

>1344_bases
TTGGTTGAAAAATCGACGGAATATAGGAAAACCAAAATTCTGGTTACTATGGGGCCATCTCTTGACGGAACTTTTGAAAA
AAGTCTTGAATTTATTGATGGAATTCGCTTTAACATGTCTCACACAAATACAAAACATATTGAAAAATATCTCGATATTT
TAAATAAAAAAAATATTGCAAAACTCATGGATTTAAAGGGAAACAAGATTAGGATAAAAAAATCAAATTTTTCAGAACTC
ATTGAAAATCAGGTTGTAAAACTTGGAAAAGATATTCTAATTTCATATATTCCAGAATGTATTAAAAAGGGCCAAAATAT
CCTGATTAATGATGGAAAAATAAAATTAATCGTTGAAAGTGTTGAAAAAGATATAACTGCACGTGTTTTAGTTGGAGGAC
ACATAAAAGAAAAAATGGGAGTAAACTTTCCTGAAGGATGCTTTCCAAACGATTTAACAGATGAAGATATAAAAAACGTG
AAATTTGCAGTTGAAAATGAATTTGAATACATTGCACTATCTTTCGTTAGAAGTGAAACGGATATTTTGAATTTAAAAAA
TTTAATTAAAGAATATTCTGGAAATTGCTCAGTTATTGCAAAAATCGAGACCAAAGAAGGATTGAAAAACATTGATGAGA
TTTTAAATCATTCGGATGGCGTAATGATTGCGAGAGGCGATCTTGGAGTTGAAGTTGCCATTGAATATCTGCCGATAATT
CAAAAAAATATTATAAAACTTGCAAATGAGAAGGGAAAACTCATAATTACTGCAACCCAGATGCTCGACTCAATGGTAAA
TAATCCGTATCCCACAAGAGCAGAAGTTACGGATGTTGCAAATGCAATATTTGATGGAACCGACTGTTTAATGCTTTCAA
ATGAAACCACAATTGGAAAATATCCAATCGAATCAATAAAAACTCTCGATAATATTGCAAGAGTTTCAGAAAATGATATT
TCAAAATACAAACAGGAAATAGAATTTGAAGAACATTCAATCTATTCAGGAATTGCCTATGCAGTTGAAGCTCTTTCAAA
AAAATTAAATCCTAAGATAGTAATTACTCCAACAACTTCGGGAAAAACTCCGCTCAGAATTTCAAAATTCCGGTTTAATT
CACCAATTATTGCCCTTGCTCCTGAAAATATAGTTTTTAGAAAATTAAGATTGGTTTGGGGAGTAATTCCAATAAAAATT
GATGAAATAGGAGATGTTGATGAAGTACTTGAAATATCTAAAAAAATTGCGAAAAATATAATTGACAGCGGTATTTACGT
TGTAACGCTCGGGCACCCAAAAGGCGAGAAAAAAACAAACGTTATAAAAGTTGAGAGCATATAA

Upstream 100 bases:

>100_bases
CACCCGCGATGTTATTATAATTTAATTTTGATATAGTTATTAAATTATTTTTCTTCTCGCATATCTAGGTAAGTATTATT
TTATATTTTGGAGGGGTATT

Downstream 100 bases:

>100_bases
TCAAATAACAGCTTGAAAAAAGGAGATTTAAATGCACCCTACAAAAACACTTCACGGAGCAAAATCAAAATTACTGGATG
GTAAAAAACTCGTTGTAGCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MVEKSTEYRKTKILVTMGPSLDGTFEKSLEFIDGIRFNMSHTNTKHIEKYLDILNKKNIAKLMDLKGNKIRIKKSNFSEL
IENQVVKLGKDILISYIPECIKKGQNILINDGKIKLIVESVEKDITARVLVGGHIKEKMGVNFPEGCFPNDLTDEDIKNV
KFAVENEFEYIALSFVRSETDILNLKNLIKEYSGNCSVIAKIETKEGLKNIDEILNHSDGVMIARGDLGVEVAIEYLPII
QKNIIKLANEKGKLIITATQMLDSMVNNPYPTRAEVTDVANAIFDGTDCLMLSNETTIGKYPIESIKTLDNIARVSENDI
SKYKQEIEFEEHSIYSGIAYAVEALSKKLNPKIVITPTTSGKTPLRISKFRFNSPIIALAPENIVFRKLRLVWGVIPIKI
DEIGDVDEVLEISKKIAKNIIDSGIYVVTLGHPKGEKKTNVIKVESI

Sequences:

>Translated_447_residues
MVEKSTEYRKTKILVTMGPSLDGTFEKSLEFIDGIRFNMSHTNTKHIEKYLDILNKKNIAKLMDLKGNKIRIKKSNFSEL
IENQVVKLGKDILISYIPECIKKGQNILINDGKIKLIVESVEKDITARVLVGGHIKEKMGVNFPEGCFPNDLTDEDIKNV
KFAVENEFEYIALSFVRSETDILNLKNLIKEYSGNCSVIAKIETKEGLKNIDEILNHSDGVMIARGDLGVEVAIEYLPII
QKNIIKLANEKGKLIITATQMLDSMVNNPYPTRAEVTDVANAIFDGTDCLMLSNETTIGKYPIESIKTLDNIARVSENDI
SKYKQEIEFEEHSIYSGIAYAVEALSKKLNPKIVITPTTSGKTPLRISKFRFNSPIIALAPENIVFRKLRLVWGVIPIKI
DEIGDVDEVLEISKKIAKNIIDSGIYVVTLGHPKGEKKTNVIKVESI
>Mature_447_residues
MVEKSTEYRKTKILVTMGPSLDGTFEKSLEFIDGIRFNMSHTNTKHIEKYLDILNKKNIAKLMDLKGNKIRIKKSNFSEL
IENQVVKLGKDILISYIPECIKKGQNILINDGKIKLIVESVEKDITARVLVGGHIKEKMGVNFPEGCFPNDLTDEDIKNV
KFAVENEFEYIALSFVRSETDILNLKNLIKEYSGNCSVIAKIETKEGLKNIDEILNHSDGVMIARGDLGVEVAIEYLPII
QKNIIKLANEKGKLIITATQMLDSMVNNPYPTRAEVTDVANAIFDGTDCLMLSNETTIGKYPIESIKTLDNIARVSENDI
SKYKQEIEFEEHSIYSGIAYAVEALSKKLNPKIVITPTTSGKTPLRISKFRFNSPIIALAPENIVFRKLRLVWGVIPIKI
DEIGDVDEVLEISKKIAKNIIDSGIYVVTLGHPKGEKKTNVIKVESI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=31.1111111111111, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=31.1111111111111, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI33286422, Length=488, Percent_Identity=31.7622950819672, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI33286420, Length=488, Percent_Identity=31.7622950819672, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=32.6530612244898, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=30.7958477508651, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=30.7958477508651, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=26.890756302521, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=26.890756302521, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787965, Length=441, Percent_Identity=32.6530612244898, Blast_Score=221, Evalue=8e-59,
Organism=Escherichia coli, GI1788160, Length=437, Percent_Identity=33.1807780320366, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=33.4016393442623, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=31.3278008298755, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=31.3278008298755, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=31.3278008298755, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=31.3278008298755, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324923, Length=485, Percent_Identity=29.8969072164948, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319279, Length=492, Percent_Identity=31.5040650406504, Blast_Score=196, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24648964, Length=429, Percent_Identity=33.7995337995338, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI28571814, Length=429, Percent_Identity=33.7995337995338, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24648966, Length=331, Percent_Identity=34.1389728096677, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=29.0650406504065, Blast_Score=189, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=33.8235294117647, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50144; Mature: 50144

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKSTEYRKTKILVTMGPSLDGTFEKSLEFIDGIRFNMSHTNTKHIEKYLDILNKKNIA
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCHH
KLMDLKGNKIRIKKSNFSELIENQVVKLGKDILISYIPECIKKGQNILINDGKIKLIVES
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEH
VEKDITARVLVGGHIKEKMGVNFPEGCFPNDLTDEDIKNVKFAVENEFEYIALSFVRSET
HHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHCEEEEECCCEEEEEEEHHCCH
DILNLKNLIKEYSGNCSVIAKIETKEGLKNIDEILNHSDGVMIARGDLGVEVAIEYLPII
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
QKNIIKLANEKGKLIITATQMLDSMVNNPYPTRAEVTDVANAIFDGTDCLMLSNETTIGK
HHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
YPIESIKTLDNIARVSENDISKYKQEIEFEEHSIYSGIAYAVEALSKKLNPKIVITPTTS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
GKTPLRISKFRFNSPIIALAPENIVFRKLRLVWGVIPIKIDEIGDVDEVLEISKKIAKNI
CCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
IDSGIYVVTLGHPKGEKKTNVIKVESI
HCCCEEEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure
MVEKSTEYRKTKILVTMGPSLDGTFEKSLEFIDGIRFNMSHTNTKHIEKYLDILNKKNIA
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCHH
KLMDLKGNKIRIKKSNFSELIENQVVKLGKDILISYIPECIKKGQNILINDGKIKLIVES
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEEEH
VEKDITARVLVGGHIKEKMGVNFPEGCFPNDLTDEDIKNVKFAVENEFEYIALSFVRSET
HHCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCHHHHHHCEEEEECCCEEEEEEEHHCCH
DILNLKNLIKEYSGNCSVIAKIETKEGLKNIDEILNHSDGVMIARGDLGVEVAIEYLPII
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
QKNIIKLANEKGKLIITATQMLDSMVNNPYPTRAEVTDVANAIFDGTDCLMLSNETTIGK
HHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
YPIESIKTLDNIARVSENDISKYKQEIEFEEHSIYSGIAYAVEALSKKLNPKIVITPTTS
CCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
GKTPLRISKFRFNSPIIALAPENIVFRKLRLVWGVIPIKIDEIGDVDEVLEISKKIAKNI
CCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHH
IDSGIYVVTLGHPKGEKKTNVIKVESI
HCCCEEEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]