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The map label for this gene is hisH

Identifier: 45358645

GI number: 45358645

Start: 1073455

End: 1074063

Strand: Direct

Name: hisH

Synonym: MMP1082

Alternate gene names: 45358645

Gene position: 1073455-1074063 (Clockwise)

Preceding gene: 45358644

Following gene: 45358646

Centisome position: 64.62

GC content: 28.74

Gene sequence:

>609_bases
ATGATAGCAATAATTGATTATGGAATGGGAAACGTTGGCTCAATTAAAAACATGATCGCAAAAATAGGTTTTGACGCTAT
TATAACCAATGATCCTGAATTAATAAGTAAAGCTACTAAATTAATTTTACCGGGAGTGGGATCTTTCGATTCAGGAATGA
CTAATTTAAAAGAGTTGGGTTTAATAGATATACTGAATAAAAAGGTTGTTCAAGAAAAAACACCCTTGTTAGGCATTTGT
TTAGGAATGCATTTATTAACCAATTCGAGTGAAGAAGGGCGTTTAAAAGGATTAGGCTTTATTAATGCAAAAACTGTTAA
ATTTAAATTATCGAATAAATTTAAAATACCCCATATGGGTTGGAACTACGTTAAATTCAGTATTAAAAATAAATTATCTG
ATAATTTAATCGAAAATTCAAGGTTTTATTTTGTGCATAGTTATTATGTAATTTGTGAAGATAAAAAGAATATACTAATG
ACTACGGAATACGAAAATGAATTTACAAGTGCTGTTTCGAAGGATAACATCTATGGAGTTCAATTTCATCCTGAAAAAAG
CCATAAGTTTGGAATGAAATTAATGGAGAATTTTATAAAACAGGCATAG

Upstream 100 bases:

>100_bases
GAAATTACCAAATAAATCATACTATGATTATCCCTCTTATGAAAATAGTAAATTTTATAAATTTTTAAGAGAAACATATA
AAAAAATAAGGGTTTACTAA

Downstream 100 bases:

>100_bases
GAATAGAATAATACATTGTTTATTATTAAAAGATGAAAGGTTAGTTAAATGTATAGGAATAGAATAATACCTTGTTTATT
ATTAAAAGATGAAAGGTTAG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 2; IGP synthase subunit hisH 2; ImGP synthase subunit hisH 2; IGPS subunit hisH 2

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MIAIIDYGMGNVGSIKNMIAKIGFDAIITNDPELISKATKLILPGVGSFDSGMTNLKELGLIDILNKKVVQEKTPLLGIC
LGMHLLTNSSEEGRLKGLGFINAKTVKFKLSNKFKIPHMGWNYVKFSIKNKLSDNLIENSRFYFVHSYYVICEDKKNILM
TTEYENEFTSAVSKDNIYGVQFHPEKSHKFGMKLMENFIKQA

Sequences:

>Translated_202_residues
MIAIIDYGMGNVGSIKNMIAKIGFDAIITNDPELISKATKLILPGVGSFDSGMTNLKELGLIDILNKKVVQEKTPLLGIC
LGMHLLTNSSEEGRLKGLGFINAKTVKFKLSNKFKIPHMGWNYVKFSIKNKLSDNLIENSRFYFVHSYYVICEDKKNILM
TTEYENEFTSAVSKDNIYGVQFHPEKSHKFGMKLMENFIKQA
>Mature_202_residues
MIAIIDYGMGNVGSIKNMIAKIGFDAIITNDPELISKATKLILPGVGSFDSGMTNLKELGLIDILNKKVVQEKTPLLGIC
LGMHLLTNSSEEGRLKGLGFINAKTVKFKLSNKFKIPHMGWNYVKFSIKNKLSDNLIENSRFYFVHSYYVICEDKKNILM
TTEYENEFTSAVSKDNIYGVQFHPEKSHKFGMKLMENFIKQA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=40, Blast_Score=147, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=35.6807511737089, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS52_METMP (P61783)

Other databases:

- EMBL:   BX950229
- RefSeq:   NP_988202.1
- ProteinModelPortal:   P61783
- SMR:   P61783
- GeneID:   2761201
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP1082
- NMPDR:   fig|267377.1.peg.1082
- HOGENOM:   HBG292341
- OMA:   GAYDQGM
- ProtClustDB:   PRK13181
- BioCyc:   MMAR267377:MMP1082-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22797; Mature: 22797

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIIDYGMGNVGSIKNMIAKIGFDAIITNDPELISKATKLILPGVGSFDSGMTNLKELG
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
LIDILNKKVVQEKTPLLGICLGMHLLTNSSEEGRLKGLGFINAKTVKFKLSNKFKIPHMG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEECCEEECCCCC
WNYVKFSIKNKLSDNLIENSRFYFVHSYYVICEDKKNILMTTEYENEFTSAVSKDNIYGV
CCEEEEEEHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCCEEEE
QFHPEKSHKFGMKLMENFIKQA
EECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAIIDYGMGNVGSIKNMIAKIGFDAIITNDPELISKATKLILPGVGSFDSGMTNLKELG
CEEEEECCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
LIDILNKKVVQEKTPLLGICLGMHLLTNSSEEGRLKGLGFINAKTVKFKLSNKFKIPHMG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEECCEEECCCCC
WNYVKFSIKNKLSDNLIENSRFYFVHSYYVICEDKKNILMTTEYENEFTSAVSKDNIYGV
CCEEEEEEHHHHHHHHHCCCEEEEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCCEEEE
QFHPEKSHKFGMKLMENFIKQA
EECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA