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The map label for this gene is hisF2 [H]

Identifier: 45358646

GI number: 45358646

Start: 1074112

End: 1074846

Strand: Direct

Name: hisF2 [H]

Synonym: MMP1083

Alternate gene names: 45358646

Gene position: 1074112-1074846 (Clockwise)

Preceding gene: 45358645

Following gene: 45358651

Centisome position: 64.66

GC content: 29.52

Gene sequence:

>735_bases
ATGTATAGGAATAGAATAATACCTTGTTTATTATTAAAAGATGAAAGGTTAGTTAAAACAGTTAAATTTAAAGATCCAAA
TTATATAGGTGATCCTTTAAATGCAGTCAGGATATTCAATGATAAAGAAGCTGATGAACTTTTTTTTATTGATATTAGTG
CTTCAAAAAGAGAGAGTATAAATTTTGATTTACTTAAAAAAATATCATCCCAAAGTTTCATGCCTCTGGGTTATGCTGGC
GGCATTAAATGTTTAAATGATGCGAAAAAGATTTTTTCCATAGGTTTTGAAAAAATCTCGTTAAATACCATTGTTCTCAA
AAATCCTGATTTGATAACGGAAATTTCTAAGATATATGGTTCTCAAAGTGTGTTAGTAACTATTGATGTTAAAAAAAATT
TTTTCGGAAAATATTATGTCTATAACCATTTGAAAAAAAAAATAACTAAATATAATCCCGTAGATTTTGCAAGAAAAATG
GAAGTTTTGGGTGCTGGAGAACTCGTAATAAACTGCGTAGATAATGATGGTGTTATGAAAGGTTATAATTTAAAATTAAT
AGGTGAAATTTCAAAAGCAGTTAAAATTCCAATCGTTGCGTTGGGTGGTGCAGGTAGTTTAAAAGATTTAGACGATGCAA
TTAATGTGGGAGCAAGTGCTGCAGGTGCAGGTAGTTTATTTATATATTATGGCCCGCATAAAGCGGTTTTAATAAACTAT
CCGAGAGGAGATTAA

Upstream 100 bases:

>100_bases
AAGCCATAAGTTTGGAATGAAATTAATGGAGAATTTTATAAAACAGGCATAGGAATAGAATAATACATTGTTTATTATTA
AAAGATGAAAGGTTAGTTAA

Downstream 100 bases:

>100_bases
TATTGCTCCAAATAAAAGCATTATACATAATTTATGATATCTCTTGTTTTTTAATCAATAAACTTTAGAATGAACTATCT
AAATTAGGTCCCTTTTGAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESINFDLLKKISSQSFMPLGYAG
GIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYGSQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKM
EVLGAGELVINCVDNDGVMKGYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
PRGD

Sequences:

>Translated_244_residues
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESINFDLLKKISSQSFMPLGYAG
GIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYGSQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKM
EVLGAGELVINCVDNDGVMKGYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
PRGD
>Mature_244_residues
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESINFDLLKKISSQSFMPLGYAG
GIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYGSQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKM
EVLGAGELVINCVDNDGVMKGYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
PRGD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=244, Percent_Identity=33.6065573770492, Blast_Score=147, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319725, Length=291, Percent_Identity=25.4295532646048, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27041; Mature: 27041

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESI
CCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
NFDLLKKISSQSFMPLGYAGGIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYG
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHC
SQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKMEVLGAGELVINCVDNDGVMK
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEE
GYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
CCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEC
PRGD
CCCC
>Mature Secondary Structure
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESI
CCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCC
NFDLLKKISSQSFMPLGYAGGIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYG
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHC
SQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKMEVLGAGELVINCVDNDGVMK
CCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEEECCCCEEE
GYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
CCCEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEC
PRGD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8939432; 12057956; 10984043 [H]