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The map label for this gene is topA [H]

Identifier: 45358519

GI number: 45358519

Start: 943765

End: 946236

Strand: Direct

Name: topA [H]

Synonym: MMP0956

Alternate gene names: 45358519

Gene position: 943765-946236 (Clockwise)

Preceding gene: 45358518

Following gene: 45358524

Centisome position: 56.81

GC content: 35.52

Gene sequence:

>2472_bases
ATGACTGGACTAATCATCTGTGAGAAACCAAACGTTGCTAAAAAAATTGCAGAAGCCCTTGGAAAGCCAAAGAAAAAATC
CCATAATTCAGTGCCATATTATGAATTAGAACGAAATGGGGAAAGTATAGTGGTAGCTTCTGCGGTAGGTCACTTATACA
CGCTCCAAGAAAAAACCAAAACTAAATTTGGAGATTACCCTGTTTACGATATCAATTGGGTTCCCGCATCAACTCTTGAT
GAAAAAAAATACATTCAAAAATATATTGATGCACTGAAAAAAGTTTCAAAAGACGCAAACAAATTCTACGTTGCAACGGA
TTGGGATATTGAAGGGGAATTGATTGGTTACCATGCACTGAATTTTTCATGCGGTCAGAAAAACGCTCACAGATTAAGAT
TTTCAAGTTTAACTAAAAAAGAAATCCTGAAAGCTTATGAAAATCCAAATGAAATAGATTTTGGACTTGTTGACGCAGGA
GACAGCAGGCACAAAATCGATTGGTATTTTGGTATAAATGTTTCAAGAGCACTCATGCAGGCAGTAAGTTCAGTTAAACG
ATGGAAAACCCTCAGTACAGGTAGGGTTCAGGGCCCAGCACTTGCTTTTTTAGTAGATAAAGAATTAGAAATTAGAAATT
TTGTTCCAACCCCATATTGGGTGCTTGAAGCTCTTTTAGATAATGACTTAATTGCAGTTCACGAGCTTGAAAAATTCTGG
GATGAAGAACAGGCAAATAAAGCTTATGAAAAGGTAAAGGGTCAAAAACAGGCAACAGTTTCAGACGTTAAAAAAAGCAT
GAAAACCATCCCTCCAAATCCGCCTTTCGATTTAGGTGCACTGCAAAGGGAAGCTCACAACATGTTTAGGTTTTCCCCTA
AAAAAACGCAAGAAGTTGCACAAAAACTTTATGAAAAAGGATACTGTTCATACCCAAGAACAAGTTCACAAAAACTTCCA
GACGACAAGGCATACATGGACGAAGTCTTGAAAAACCTTTCAAAAAATAAAAATTATAAGCCATACATTGAAACAATTTT
AAATGAAAATAGAAAACCGATTTCAGGTAAAAATGACGACCCTGCACACCCTGCGGTACACGCAGTAGATGTTCCAAAGG
AAAAACTGCCCGATGATGAATTAAAGTTATACGAACTTATTGCAAGAAGAACAATTGCACTTTACTGGGATAACGCTAAA
AGGGAATATTCAAAAATCAATCTCGATATTAACGGCGAAACATTTAAATTAAGCGGTTCAAGAACCGTTGAAGAAGGATG
GCATGAAATATATTACTATACTAAATTCGATGAAGTAGAACTCCCAGAATTAAAGAAAAATGACGTAATAAATGTAAATA
ACATTAATTTCGAAGCAAAAGAGACGAAGCCTCCAAAAAGATACACGATGGCTTCGATTATCAAGGAACTTGAAAAAAGA
AAACTCGGAACAAAAGCTACAAGAGCAGATATCCTCGAAAAACTAACCAAACGAGGTTATGTAATTGAAGATGGTTCTTT
AACGGTTACAGATCTTGGAATTGGAGTTACCGAAACTTTAAGAAAATACTGTCCAGAAATTGTTGAAGAAACCCTTACAA
GAGACCTTGAAGATAAATTAGAGCTCATTCAGGACAAAAAAATCAAAAAAGAAGAAGTTATTCAGGAAACTAAAGACAAA
CTTACAAAAATCCTTGGAGAGTTCCGTTTAAAAGAAAAAGAAATCGGAACTGAACTTGTAGATAAACTCGATTCAACCAA
TAGGTCCTTACAAATAATTGGAAAATGTAAATGTGGCGGAGACATGATTATCATCCGTACAAAGGGCAAAAAAAGATTTG
TTGGATGCAGTAACTATCCTGACTGTGAAGTTACATTCCCACTCCCGCAAAAGGGAAGAATCAAGGTATTAAACGAAGTC
TGTGAAAAATGCCAAAATCCAGTAATCGGGCTTGATAGAATAAAAATTTGTGTAAATCCCGACTGCAGTACGAGAATTTC
AGAAGAAGATAAAAAAGAAATTGAAAAAGCTGAAAAGGAAGAAAAAATTTGTCCAAAATGCGGCAATAAACTTTTGATTA
AAAAAGGACCTTATGGAGTTTTCAGAGGCTGTGAAAACTACCCAAAATGTAAATATACTGAAAAATTAAACGGGGAATCC
AATGAAAAAGAAATCGTTGGAAAATGCCCAAAATGCGGAAGCGACCTTTATAAAAGAAAAGGACGATTTGGAGAGTTTAT
CGGATGCAGTAACTACCCAAAATGTAGGCATACTGAAAAGATAGACAAGAAAAAAGAAGATACGCCGGGAGAAACTAAGG
CAGAAACAAAGAAAGAAGCAAAACCAAAAACTACTAAAAAAGCAGCTCCAAAAAAGACCGCAACTAAAACTACTAAAACT
ACTGCCAAAAAGACGGCTACGAAAAAAACCACAACTAAAAAAGCAACTGCAAAGAAAACCGCAGAAAAATAA

Upstream 100 bases:

>100_bases
CATGTTGCAAAAAGAATTTCAGATATTCCATTATTATTGAAAGAAATTATTTAAACTGCTTTTTTCAATTATTTTATTAT
TAATTTAAAACGTGAAAAAA

Downstream 100 bases:

>100_bases
ATTAAACACTAAATATTAAAAATAATCCTAAAATTATCAATATAATTCCTGAGATATATGTTATAAATTTCTTTTTTACT
ATTTTTTCAACTATTTGTTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 823; Mature: 822

Protein sequence:

>823_residues
MTGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTKTKFGDYPVYDINWVPASTLD
EKKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHALNFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAG
DSRHKIDWYFGINVSRALMQAVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFW
DEEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVAQKLYEKGYCSYPRTSSQKLP
DDKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDDPAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAK
REYSKINLDINGETFKLSGSRTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKR
KLGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKLELIQDKKIKKEEVIQETKDK
LTKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGGDMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEV
CEKCQNPVIGLDRIKICVNPDCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGES
NEKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEAKPKTTKKAAPKKTATKTTKT
TAKKTATKKTTTKKATAKKTAEK

Sequences:

>Translated_823_residues
MTGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTKTKFGDYPVYDINWVPASTLD
EKKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHALNFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAG
DSRHKIDWYFGINVSRALMQAVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFW
DEEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVAQKLYEKGYCSYPRTSSQKLP
DDKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDDPAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAK
REYSKINLDINGETFKLSGSRTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKR
KLGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKLELIQDKKIKKEEVIQETKDK
LTKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGGDMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEV
CEKCQNPVIGLDRIKICVNPDCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGES
NEKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEAKPKTTKKAAPKKTATKTTKT
TAKKTATKKTTTKKATAKKTAEK
>Mature_822_residues
TGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTKTKFGDYPVYDINWVPASTLDE
KKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHALNFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAGD
SRHKIDWYFGINVSRALMQAVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFWD
EEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVAQKLYEKGYCSYPRTSSQKLPD
DKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDDPAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAKR
EYSKINLDINGETFKLSGSRTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKRK
LGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKLELIQDKKIKKEEVIQETKDKL
TKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGGDMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEVC
EKCQNPVIGLDRIKICVNPDCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGESN
EKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEAKPKTTKKAAPKKTATKTTKTT
AKKTATKKTTTKKATAKKTAEK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=693, Percent_Identity=26.1183261183261, Blast_Score=193, Evalue=5e-49,
Organism=Homo sapiens, GI4507635, Length=776, Percent_Identity=25.2577319587629, Blast_Score=184, Evalue=4e-46,
Organism=Escherichia coli, GI1787529, Length=819, Percent_Identity=28.6935286935287, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788061, Length=615, Percent_Identity=25.5284552845528, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI48994931, Length=87, Percent_Identity=50.5747126436782, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=630, Percent_Identity=27.3015873015873, Blast_Score=192, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI32563869, Length=779, Percent_Identity=25.6739409499358, Blast_Score=174, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323263, Length=636, Percent_Identity=24.685534591195, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24585251, Length=701, Percent_Identity=24.3937232524964, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24640096, Length=676, Percent_Identity=25.7396449704142, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 93929; Mature: 93798

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTK
CCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHH
TKFGDYPVYDINWVPASTLDEKKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHAL
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEE
NFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAGDSRHKIDWYFGINVSRALMQ
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHH
AVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFW
HHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
DEEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QKLYEKGYCSYPRTSSQKLPDDKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDD
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCC
PAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAKREYSKINLDINGETFKLSGS
CCCCCEEEECCCHHHCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEECCCEEEECCC
RTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKR
CCHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHH
KLGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKL
HCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELIQDKKIKKEEVIQETKDKLTKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
DMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEVCEKCQNPVIGLDRIKICVNP
CEEEEEECCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEECCEEEEEECC
DCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGES
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCHHHCCCCC
NEKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEA
CCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHC
KPKTTKKAAPKKTATKTTKTTAKKTATKKTTTKKATAKKTAEK
CCCCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTK
CCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCEEEEEEHHHHHHHHHHHHH
TKFGDYPVYDINWVPASTLDEKKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHAL
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEE
NFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAGDSRHKIDWYFGINVSRALMQ
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHH
AVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFW
HHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
DEEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVA
CHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QKLYEKGYCSYPRTSSQKLPDDKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDD
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCC
PAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAKREYSKINLDINGETFKLSGS
CCCCCEEEECCCHHHCCCHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEECCCEEEECCC
RTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKR
CCHHCCCCEEEEEEECCCCCCCCCCCCCEEEECCCCEEECCCCCCHHHHHHHHHHHHHHH
KLGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKL
HCCCHHHHHHHHHHHHHCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELIQDKKIKKEEVIQETKDKLTKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
DMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEVCEKCQNPVIGLDRIKICVNP
CEEEEEECCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEECCEEEEEECC
DCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGES
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCHHHCCCCC
NEKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEA
CCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHC
KPKTTKKAAPKKTATKTTKTTAKKTATKKTTTKKATAKKTAEK
CCCCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]