Click here to switch to the map view.

The map label for this gene is cofE [H]

Identifier: 45358500

GI number: 45358500

Start: 924790

End: 925536

Strand: Direct

Name: cofE [H]

Synonym: MMP0937

Alternate gene names: 45358500

Gene position: 924790-925536 (Clockwise)

Preceding gene: 45358499

Following gene: 45358508

Centisome position: 55.67

GC content: 34.54

Gene sequence:

>747_bases
ATGATAAAAGAAAGAGTAAAAATGGAAGTAATAGGCCTTGAAATTCCATTAATCAGTGGAAATGAAGATTATACGTTAGC
AGAGCTTATTTCCACATACCCGCTTGAAGATAAAGATGTAATAGTTATTGCAGAAACTGTTGTTTCAAAAATTGAAAAGA
ATGTGATTTTAAAAAATGAAATAACCCCGTCCAATGAAGCTATGGAATTATCCAAAAAACTTGGGAAAGAACCAGAAGTA
GTTCAGGTCATTTTAGACGAATCAAACGAAATCGTAAAATTAGGACCTAATTTTATAATAACTGAAACTAAACACGGATT
TGTTTGTGCAAACAGTGGTGTTGATGAAAGCAACACGTCAAAAGGAATAAAACCACTACCTAAAAATCCGGATAAAAGTG
CAGAAGAAATTAGAATGGGAATTGAAAAAATTACTGGAAAAAAAGTTGGCGTAATTATTAACGACAGTATGGGCAGACCA
TTTAGAAAAGGTTCATGCGGTATTGCAATAGGAGTTAGTGGAGTTTGTGGACTTTGGGATAGAAAAGGGGAAAAAGACTT
ATTTGGAAGGGAATTAAAAACAACAGAGGTTGGAATTGCAGATGAACTTGCAGCAACTGCTTCTGCTGTCATGGGGCAGT
CCAATGAGGGAATTCCCCTTGTAATTATTAGAAATGCGCCTGTTCCATTTACGAATGGAACTGGAAAAGAATTAATCAGA
AAAAAAGAAGAAGATGTATTCAGATAA

Upstream 100 bases:

>100_bases
CAGGGTGCAGTTTCCTTTGAGATATGGACAGGAATAAAACCAGACATATTCGTGATGAAGAAAAGTATTATTTCAAAAAT
TTAAAAAATCGTGGGAAAAA

Downstream 100 bases:

>100_bases
CTGAATATTTTTAATTTAATTATTTATCCATACTTTTTTAATTTTTTATTTAATTACCCAATAAGTGAAAAATATGGGGT
AAATGCAATAATTACAATCA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNEITPSNEAMELSKKLGKEPEV
VQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTSKGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRP
FRKGSCGIAIGVSGVCGLWDRKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
KKEEDVFR

Sequences:

>Translated_248_residues
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNEITPSNEAMELSKKLGKEPEV
VQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTSKGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRP
FRKGSCGIAIGVSGVCGLWDRKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
KKEEDVFR
>Mature_248_residues
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNEITPSNEAMELSKKLGKEPEV
VQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTSKGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRP
FRKGSCGIAIGVSGVCGLWDRKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
KKEEDVFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 26990; Mature: 26990

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNE
CCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCEEEECC
ITPSNEAMELSKKLGKEPEVVQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTS
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCC
KGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRPFRKGSCGIAIGVSGVCGLWD
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
RKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHH
KKEEDVFR
HHHHHCCC
>Mature Secondary Structure
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNE
CCCCCEEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCEEEECC
ITPSNEAMELSKKLGKEPEVVQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTS
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCEEEEECCCCEEEECCCCCCCCCC
KGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRPFRKGSCGIAIGVSGVCGLWD
CCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
RKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
CCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHH
KKEEDVFR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA