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The map label for this gene is nifN [H]

Identifier: 45358422

GI number: 45358422

Start: 849999

End: 851375

Strand: Direct

Name: nifN [H]

Synonym: MMP0859

Alternate gene names: 45358422

Gene position: 849999-851375 (Clockwise)

Preceding gene: 45358421

Following gene: 45358423

Centisome position: 51.17

GC content: 38.2

Gene sequence:

>1377_bases
ATGACTGAAAAAAAATTTGCTGTAATAAATCCATGTAAAATATGCCAGCCAATGGGTGCAATATTTGCATTAATGGGTGT
AAAGAATTCAATGCCGTTAATACACGGATCGCACGGATGCAGCACCTACATGAGATTCCAACTTGGACGTCATTTTAAAG
AAGCTGTAAACGTGGCATCTTCATCAATGAGTGAGGCAAATGCTGTACTAGGTGGTGAAGAAAATTTAAAACATGCGATC
AGAAACGTGAGCAAAATTTATGGTCCTGAAATAATTGGTGTAACTACAAGCTGTCTTAGTGAAACTATTGGTGACGACGT
TGAAGGGATCATCCGGTTATTCAAGACACAAAATGATGATTTACCCGAAATAGTCCCGATATCAACCCCGAGTTATAAAC
ATACACATGTAGAAGGATACGATTCAACAGTTAAGGCATTGGTTGAAAATTTAGCAACTCCATCAAAGCCAAATTCCAAG
TTAAACGTGATTACTGGAATGGTTTCTCCTGGAGACATTGCAGAAACCAAAAGGATTTTACGTGAAATGGGTATAGAATC
AATAATTCTAACCGATACTTCGGAATCTATGGACGCCCCTTTCGATGGAGAAGTTTCATTCGTGTCATCTGCAGGAACTA
CTGTGGAAGAACTTAAAGACACTGCAAATTCATTTGCAACGATTTCACTCTGTAAGCATGCAAATTCCGCCGCCAAATTC
CTCCATGAAGCTTACGGAATAAACTCTGTGTCACCATATCTTCCAATAGGACTGCAGAGTACTGATGAATTTATGAATGA
ACTTTGTAAACTTACGAATAAAGAGATACCTGAACAGATAACTAGAGAACGTGGAAAATTAATCGATGCCATGATCGACG
CCCACCAACATAATTTTGGAAGAAAAGTTGCAATCTACGGCGATCCCGATGTCGTAGTTGGATTTACAAGATTTGTTTTG
GAACTTGGAATGATTCCTTCAATAGTCTGTACAGGAAGCGAAAGTAAAACTTTCGTTGAAGACATCAAAGCAGTAACTAA
AGATAGCAAACATAAACCAGTAATCTTAGCTCCCGGGGATCTTTACGATCTCCACCAAGAAATTAAAAAATCAAAAGTGG
ATCTTTTAATTGGAAATTCGTACGGTTCAAGAATTGCAGGTGCCGAAAATATTCCATTAATTAGGATGGGTTTCCCAATC
TATGATAGAGTTGGCGCTCAAAGAATAACTTCTTTAGGCTATACTGCAGGAATTAGACTTGTAGATACTATAACCAATAC
AATACTCGACAGATACTTTGACGAGTTTGGATGGGCATTAAACGAAAATACAGAGGTAGACCATGGAGAGTTTGAAAGTA
GCAATTGCATCAACTGA

Upstream 100 bases:

>100_bases
CAGGTTATCAGGGCTTCATAAATTTTGCAAGAGTTGTACATACCGCAGTTATGACGCCAATTTGGAGACTTTCTAGAAAA
TGATGATTTAGAGGAATATT

Downstream 100 bases:

>100_bases
TGGTGTAAATGTCAACATGCACTTCGGAGCTGCCACACATTTTCTTATTTTTGAAATAAAAGATAATGTAGCAGAATTTT
TAGAATTTAGGGAAAACCCA

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTEKKFAVINPCKICQPMGAIFALMGVKNSMPLIHGSHGCSTYMRFQLGRHFKEAVNVASSSMSEANAVLGGEENLKHAI
RNVSKIYGPEIIGVTTSCLSETIGDDVEGIIRLFKTQNDDLPEIVPISTPSYKHTHVEGYDSTVKALVENLATPSKPNSK
LNVITGMVSPGDIAETKRILREMGIESIILTDTSESMDAPFDGEVSFVSSAGTTVEELKDTANSFATISLCKHANSAAKF
LHEAYGINSVSPYLPIGLQSTDEFMNELCKLTNKEIPEQITRERGKLIDAMIDAHQHNFGRKVAIYGDPDVVVGFTRFVL
ELGMIPSIVCTGSESKTFVEDIKAVTKDSKHKPVILAPGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPI
YDRVGAQRITSLGYTAGIRLVDTITNTILDRYFDEFGWALNENTEVDHGEFESSNCIN

Sequences:

>Translated_458_residues
MTEKKFAVINPCKICQPMGAIFALMGVKNSMPLIHGSHGCSTYMRFQLGRHFKEAVNVASSSMSEANAVLGGEENLKHAI
RNVSKIYGPEIIGVTTSCLSETIGDDVEGIIRLFKTQNDDLPEIVPISTPSYKHTHVEGYDSTVKALVENLATPSKPNSK
LNVITGMVSPGDIAETKRILREMGIESIILTDTSESMDAPFDGEVSFVSSAGTTVEELKDTANSFATISLCKHANSAAKF
LHEAYGINSVSPYLPIGLQSTDEFMNELCKLTNKEIPEQITRERGKLIDAMIDAHQHNFGRKVAIYGDPDVVVGFTRFVL
ELGMIPSIVCTGSESKTFVEDIKAVTKDSKHKPVILAPGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPI
YDRVGAQRITSLGYTAGIRLVDTITNTILDRYFDEFGWALNENTEVDHGEFESSNCIN
>Mature_457_residues
TEKKFAVINPCKICQPMGAIFALMGVKNSMPLIHGSHGCSTYMRFQLGRHFKEAVNVASSSMSEANAVLGGEENLKHAIR
NVSKIYGPEIIGVTTSCLSETIGDDVEGIIRLFKTQNDDLPEIVPISTPSYKHTHVEGYDSTVKALVENLATPSKPNSKL
NVITGMVSPGDIAETKRILREMGIESIILTDTSESMDAPFDGEVSFVSSAGTTVEELKDTANSFATISLCKHANSAAKFL
HEAYGINSVSPYLPIGLQSTDEFMNELCKLTNKEIPEQITRERGKLIDAMIDAHQHNFGRKVAIYGDPDVVVGFTRFVLE
LGMIPSIVCTGSESKTFVEDIKAVTKDSKHKPVILAPGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPIY
DRVGAQRITSLGYTAGIRLVDTITNTILDRYFDEFGWALNENTEVDHGEFESSNCIN

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50134; Mature: 50003

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKKFAVINPCKICQPMGAIFALMGVKNSMPLIHGSHGCSTYMRFQLGRHFKEAVNVAS
CCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
SSMSEANAVLGGEENLKHAIRNVSKIYGPEIIGVTTSCLSETIGDDVEGIIRLFKTQNDD
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCC
LPEIVPISTPSYKHTHVEGYDSTVKALVENLATPSKPNSKLNVITGMVSPGDIAETKRIL
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
REMGIESIILTDTSESMDAPFDGEVSFVSSAGTTVEELKDTANSFATISLCKHANSAAKF
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LHEAYGINSVSPYLPIGLQSTDEFMNELCKLTNKEIPEQITRERGKLIDAMIDAHQHNFG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RKVAIYGDPDVVVGFTRFVLELGMIPSIVCTGSESKTFVEDIKAVTKDSKHKPVILAPGD
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCC
LYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPIYDRVGAQRITSLGYTAGIRL
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
VDTITNTILDRYFDEFGWALNENTEVDHGEFESSNCIN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEKKFAVINPCKICQPMGAIFALMGVKNSMPLIHGSHGCSTYMRFQLGRHFKEAVNVAS
CCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
SSMSEANAVLGGEENLKHAIRNVSKIYGPEIIGVTTSCLSETIGDDVEGIIRLFKTQNDD
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCC
LPEIVPISTPSYKHTHVEGYDSTVKALVENLATPSKPNSKLNVITGMVSPGDIAETKRIL
CCCEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
REMGIESIILTDTSESMDAPFDGEVSFVSSAGTTVEELKDTANSFATISLCKHANSAAKF
HHCCCCEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
LHEAYGINSVSPYLPIGLQSTDEFMNELCKLTNKEIPEQITRERGKLIDAMIDAHQHNFG
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RKVAIYGDPDVVVGFTRFVLELGMIPSIVCTGSESKTFVEDIKAVTKDSKHKPVILAPGD
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCC
LYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPIYDRVGAQRITSLGYTAGIRL
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
VDTITNTILDRYFDEFGWALNENTEVDHGEFESSNCIN
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309 [H]