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The map label for this gene is nifE

Identifier: 45358421

GI number: 45358421

Start: 848539

End: 849981

Strand: Direct

Name: nifE

Synonym: MMP0858

Alternate gene names: 45358421

Gene position: 848539-849981 (Clockwise)

Preceding gene: 45358420

Following gene: 45358422

Centisome position: 51.08

GC content: 37.91

Gene sequence:

>1443_bases
ATGGTTTTGAACCTTGATACAGAAAACCGGAAAATGCAAGATGGAAATAACGATGATGATTTTGACTTAGAAGTCAAAAT
CCCAAATTCCATTTTTGAAAAATTAAAATCTATTGAAGCGTTAAAAGCCAGAGAATCACAAATGTGTGTTTCTGGAAAAG
ACGATTCAATTCCCACATGTGACAAAAACTCAACCCCAGGAATGATTACCCAGAGATCCTGCGTATACGGGGGAGCAAGA
GTTGTTCTAATGCCTATAACTGATGCAGTACATCTCGTACACGGGCCAATAGGCTGCGCAGCATGCACTTGGGATATTCG
TGGAAGTAAATCTACAGGAGATAAACTTTACAAAAACGGATTCTCAACTGATTTGCAAGAGAAAGATATTGTATTTGGTG
GAGAAAAAAAGCTCTATGAATCCATTTTGGAAGTAAATAAGCTTTACCACCCTGGTGCAATATTTGTTTACTCTACTTGT
GTAGTGGGTTTGATTGGAGATGATCTTAAAGCAGTTTGTAGACAGGCACAAGAAGCAACGGGCTGCAGAGTTATTCCGGT
ACAGTCAGAAGGGTTCAAAAGCTTTAATAAAACTGCAGGTCACAAACTCGCATGTGACGCAATGCTTGATTACGTAATTG
GCACAGAAGAACCAGAAGAAGAGCACCCCTACAGTATTAATATAATTGGAGAATTCAATGTTGCAGGAGATCTCTGGGGA
ATTATTCCATTATATGAAAAAATGGGTGTAAAAGTACACACTGCAATTACTGGAGACTCAACAGTCGCTAAAGTTGCAAG
TGCGCACAGATCTAAATTAAATATAGTTCAATGCCAAAAATCATCCAATTATTTAGCCGCTCAAATGGAAAAGAAATACG
GAATACCAAGCATTAAAGTAAATTTCTTTGGGCTCGATGAAACTACAAAGTCATTAAGGGCTGTTGCAGAATTTTTCGGC
GACGAAGAAATGATTAAAAGAACTGAAGAACTCATAAAATCAGAAATTAAAAATCTTAGAGATGAAATTTCTGAATACAA
AAAAGATCTATCAGGTAGAACCGTGGCGATATATTCCGGAGCCCACAAATCTTGGGCACTGGTAAGCGCATTTGGTGAAC
TCGATATGGAAATTATCATGAGTGGTACCCAAAACGGAAAACCCGAAGATTACCAACAAATTAGAGATCACGTCTGCGAA
GGTACATTAATTGTGGACGATGCAAGTTCAATGGAACTTGTACAGCTTCTCAAAGAATACAAACCTGACATATTAATTTC
TGGAGCTAAAGAAAAATACTTATCACTGAAAAGTGGAATTCCTCACTGTGATTTCAATCACGACAGGATAACTGCCTTTT
CAGGTTATCAGGGCTTCATAAATTTTGCAAGAGTTGTACATACCGCAGTTATGACGCCAATTTGGAGACTTTCTAGAAAA
TGA

Upstream 100 bases:

>100_bases
TTGAACCTTGTCGATGGAATTACAAATACAATTCTCGATAAATACTACGAAACTCAAGACTGGAAACTCCAACAGTAAGC
CCAAAATAAAGAGGAATACT

Downstream 100 bases:

>100_bases
TGATTTAGAGGAATATTATGACTGAAAAAAAATTTGCTGTAATAAATCCATGTAAAATATGCCAGCCAATGGGTGCAATA
TTTGCATTAATGGGTGTAAA

Product: nitrogenase MoFe cofactor biosynthesis protein NifE

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVLNLDTENRKMQDGNNDDDFDLEVKIPNSIFEKLKSIEALKARESQMCVSGKDDSIPTCDKNSTPGMITQRSCVYGGAR
VVLMPITDAVHLVHGPIGCAACTWDIRGSKSTGDKLYKNGFSTDLQEKDIVFGGEKKLYESILEVNKLYHPGAIFVYSTC
VVGLIGDDLKAVCRQAQEATGCRVIPVQSEGFKSFNKTAGHKLACDAMLDYVIGTEEPEEEHPYSINIIGEFNVAGDLWG
IIPLYEKMGVKVHTAITGDSTVAKVASAHRSKLNIVQCQKSSNYLAAQMEKKYGIPSIKVNFFGLDETTKSLRAVAEFFG
DEEMIKRTEELIKSEIKNLRDEISEYKKDLSGRTVAIYSGAHKSWALVSAFGELDMEIIMSGTQNGKPEDYQQIRDHVCE
GTLIVDDASSMELVQLLKEYKPDILISGAKEKYLSLKSGIPHCDFNHDRITAFSGYQGFINFARVVHTAVMTPIWRLSRK

Sequences:

>Translated_480_residues
MVLNLDTENRKMQDGNNDDDFDLEVKIPNSIFEKLKSIEALKARESQMCVSGKDDSIPTCDKNSTPGMITQRSCVYGGAR
VVLMPITDAVHLVHGPIGCAACTWDIRGSKSTGDKLYKNGFSTDLQEKDIVFGGEKKLYESILEVNKLYHPGAIFVYSTC
VVGLIGDDLKAVCRQAQEATGCRVIPVQSEGFKSFNKTAGHKLACDAMLDYVIGTEEPEEEHPYSINIIGEFNVAGDLWG
IIPLYEKMGVKVHTAITGDSTVAKVASAHRSKLNIVQCQKSSNYLAAQMEKKYGIPSIKVNFFGLDETTKSLRAVAEFFG
DEEMIKRTEELIKSEIKNLRDEISEYKKDLSGRTVAIYSGAHKSWALVSAFGELDMEIIMSGTQNGKPEDYQQIRDHVCE
GTLIVDDASSMELVQLLKEYKPDILISGAKEKYLSLKSGIPHCDFNHDRITAFSGYQGFINFARVVHTAVMTPIWRLSRK
>Mature_480_residues
MVLNLDTENRKMQDGNNDDDFDLEVKIPNSIFEKLKSIEALKARESQMCVSGKDDSIPTCDKNSTPGMITQRSCVYGGAR
VVLMPITDAVHLVHGPIGCAACTWDIRGSKSTGDKLYKNGFSTDLQEKDIVFGGEKKLYESILEVNKLYHPGAIFVYSTC
VVGLIGDDLKAVCRQAQEATGCRVIPVQSEGFKSFNKTAGHKLACDAMLDYVIGTEEPEEEHPYSINIIGEFNVAGDLWG
IIPLYEKMGVKVHTAITGDSTVAKVASAHRSKLNIVQCQKSSNYLAAQMEKKYGIPSIKVNFFGLDETTKSLRAVAEFFG
DEEMIKRTEELIKSEIKNLRDEISEYKKDLSGRTVAIYSGAHKSWALVSAFGELDMEIIMSGTQNGKPEDYQQIRDHVCE
GTLIVDDASSMELVQLLKEYKPDILISGAKEKYLSLKSGIPHCDFNHDRITAFSGYQGFINFARVVHTAVMTPIWRLSRK

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor)

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFE_METMP (P71528)

Other databases:

- EMBL:   U75887
- EMBL:   BX950229
- PIR:   T10095
- RefSeq:   NP_987978.1
- ProteinModelPortal:   P71528
- SMR:   P71528
- GeneID:   2762315
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0858
- NMPDR:   fig|267377.1.peg.858
- HOGENOM:   HBG392742
- OMA:   YTWDIRG
- ProtClustDB:   CLSK878516
- BioCyc:   MMAR267377:MMP0858-MONOMER
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973
- TIGRFAMs:   TIGR01283

Pfam domain/function: PF00148 Oxidored_nitro

EC number: 1.18.6.1

Molecular weight: Translated: 53289; Mature: 53289

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLNLDTENRKMQDGNNDDDFDLEVKIPNSIFEKLKSIEALKARESQMCVSGKDDSIPTC
CEEECCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
DKNSTPGMITQRSCVYGGARVVLMPITDAVHLVHGPIGCAACTWDIRGSKSTGDKLYKNG
CCCCCCCCEEECCEECCCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCC
FSTDLQEKDIVFGGEKKLYESILEVNKLYHPGAIFVYSTCVVGLIGDDLKAVCRQAQEAT
CCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GCRVIPVQSEGFKSFNKTAGHKLACDAMLDYVIGTEEPEEEHPYSINIIGEFNVAGDLWG
CCEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHH
IIPLYEKMGVKVHTAITGDSTVAKVASAHRSKLNIVQCQKSSNYLAAQMEKKYGIPSIKV
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEE
NFFGLDETTKSLRAVAEFFGDEEMIKRTEELIKSEIKNLRDEISEYKKDLSGRTVAIYSG
EEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
AHKSWALVSAFGELDMEIIMSGTQNGKPEDYQQIRDHVCEGTLIVDDASSMELVQLLKEY
CCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHC
KPDILISGAKEKYLSLKSGIPHCDFNHDRITAFSGYQGFINFARVVHTAVMTPIWRLSRK
CCCEEEECCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLNLDTENRKMQDGNNDDDFDLEVKIPNSIFEKLKSIEALKARESQMCVSGKDDSIPTC
CEEECCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
DKNSTPGMITQRSCVYGGARVVLMPITDAVHLVHGPIGCAACTWDIRGSKSTGDKLYKNG
CCCCCCCCEEECCEECCCCEEEEEECCHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHCC
FSTDLQEKDIVFGGEKKLYESILEVNKLYHPGAIFVYSTCVVGLIGDDLKAVCRQAQEAT
CCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GCRVIPVQSEGFKSFNKTAGHKLACDAMLDYVIGTEEPEEEHPYSINIIGEFNVAGDLWG
CCEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHH
IIPLYEKMGVKVHTAITGDSTVAKVASAHRSKLNIVQCQKSSNYLAAQMEKKYGIPSIKV
HHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCCEEEE
NFFGLDETTKSLRAVAEFFGDEEMIKRTEELIKSEIKNLRDEISEYKKDLSGRTVAIYSG
EEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
AHKSWALVSAFGELDMEIIMSGTQNGKPEDYQQIRDHVCEGTLIVDDASSMELVQLLKEY
CCCCHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHC
KPDILISGAKEKYLSLKSGIPHCDFNHDRITAFSGYQGFINFARVVHTAVMTPIWRLSRK
CCCEEEECCHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309