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The map label for this gene is radB [H]

Identifier: 45358180

GI number: 45358180

Start: 612707

End: 613357

Strand: Direct

Name: radB [H]

Synonym: MMP0617

Alternate gene names: 45358180

Gene position: 612707-613357 (Clockwise)

Preceding gene: 45358179

Following gene: 45358181

Centisome position: 36.88

GC content: 31.64

Gene sequence:

>651_bases
ATGCTTGAAGAACTTTTGAACGGAAATATCGAAAAAAAGACGATAACTCAAATTTACGGGCCTCCTGGCGTTGGAAAAAC
AAATATTTGTATAATTTCAATGTTAAAAGCAATTGAAAATGGCAAAAATGTGGTTTACATCGATACAGAAGGGAGTTTAT
CAATTGAACGAATAAAACAGCTGTCTGGAAAGGATTGTGATGAATTACTTAAAAATATAATAATTTATGAACCGTCTTCG
TTTGAAGAACAGTCTGAAGCTTTAGAAAAGATATTTCTTCTTGAAAATGTGGGGCTTATAATCATTGACGGCATTGTTTC
GCTTTATAGACTTGAACTCTGTGACAAAATCAATGAAAATACTAAATTAAATAGGATGCTTGGAAAACAGATTTCAAATC
TTTTAAAAGTTTCCCGACAGAAAAATTCTGGAATTTTGATTACGAATCAGGTGAAAGACTCGATAAATGGTATCGAACCT
GCTGGAGGGAGGCTTTTAGAATATTGGAGTAAATCAATCATAAAAATTGAAAAAAGTGAATCTATTAGAAAACTTACCTT
GGAAAAACACAGACACGCAAAAGAAGGCGAGAATTTAAGGTTCAAAATACTTCAGAATGGTCTTGAAATAATAAATAAGT
CCTATCAATAA

Upstream 100 bases:

>100_bases
CATTTAAGTAAACCTCTCGTGTTATACGACTTTGATCTGGAAAATATAAGGTAAATTATTTATTAGATAATTATACATTT
CATTATATCAATGTGATTTT

Downstream 100 bases:

>100_bases
ACTTTTTTCTAATTATATTAATTTAAACCTGTGCTTTATAAAAAAACTATATATATCATGTTTCACACATATGTGTAAGT
AGTCATTTTATAAGGGGGGA

Product: DNA repair and recombination protein RadB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLEELLNGNIEKKTITQIYGPPGVGKTNICIISMLKAIENGKNVVYIDTEGSLSIERIKQLSGKDCDELLKNIIIYEPSS
FEEQSEALEKIFLLENVGLIIIDGIVSLYRLELCDKINENTKLNRMLGKQISNLLKVSRQKNSGILITNQVKDSINGIEP
AGGRLLEYWSKSIIKIEKSESIRKLTLEKHRHAKEGENLRFKILQNGLEIINKSYQ

Sequences:

>Translated_216_residues
MLEELLNGNIEKKTITQIYGPPGVGKTNICIISMLKAIENGKNVVYIDTEGSLSIERIKQLSGKDCDELLKNIIIYEPSS
FEEQSEALEKIFLLENVGLIIIDGIVSLYRLELCDKINENTKLNRMLGKQISNLLKVSRQKNSGILITNQVKDSINGIEP
AGGRLLEYWSKSIIKIEKSESIRKLTLEKHRHAKEGENLRFKILQNGLEIINKSYQ
>Mature_216_residues
MLEELLNGNIEKKTITQIYGPPGVGKTNICIISMLKAIENGKNVVYIDTEGSLSIERIKQLSGKDCDELLKNIIIYEPSS
FEEQSEALEKIFLLENVGLIIIDGIVSLYRLELCDKINENTKLNRMLGKQISNLLKVSRQKNSGILITNQVKDSINGIEP
AGGRLLEYWSKSIIKIEKSESIRKLTLEKHRHAKEGENLRFKILQNGLEIINKSYQ

Specific function: Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family. RadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23238219, Length=199, Percent_Identity=26.6331658291457, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI46255039, Length=188, Percent_Identity=27.1276595744681, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI19924117, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI10835029, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321027, Length=198, Percent_Identity=28.2828282828283, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651285, Length=227, Percent_Identity=25.5506607929515, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17864108, Length=198, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011939
- InterPro:   IPR020588 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 24456; Mature: 24456

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEELLNGNIEKKTITQIYGPPGVGKTNICIISMLKAIENGKNVVYIDTEGSLSIERIKQ
CCHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
LSGKDCDELLKNIIIYEPSSFEEQSEALEKIFLLENVGLIIIDGIVSLYRLELCDKINEN
CCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
TKLNRMLGKQISNLLKVSRQKNSGILITNQVKDSINGIEPAGGRLLEYWSKSIIKIEKSE
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHHEEECCHH
SIRKLTLEKHRHAKEGENLRFKILQNGLEIINKSYQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLEELLNGNIEKKTITQIYGPPGVGKTNICIISMLKAIENGKNVVYIDTEGSLSIERIKQ
CCHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
LSGKDCDELLKNIIIYEPSSFEEQSEALEKIFLLENVGLIIIDGIVSLYRLELCDKINEN
CCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
TKLNRMLGKQISNLLKVSRQKNSGILITNQVKDSINGIEPAGGRLLEYWSKSIIKIEKSE
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHHEEECCHH
SIRKLTLEKHRHAKEGENLRFKILQNGLEIINKSYQ
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA