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The map label for this gene is leuB [H]

Identifier: 45358102

GI number: 45358102

Start: 543913

End: 544905

Strand: Direct

Name: leuB [H]

Synonym: MMP0539

Alternate gene names: 45358102

Gene position: 543913-544905 (Clockwise)

Preceding gene: 45358101

Following gene: 45358103

Centisome position: 32.74

GC content: 37.97

Gene sequence:

>993_bases
ATGCATAAGATTTGCGTAATCGAAGGCGATGGGATTGGAAAAGAAGTGGTTCCTGCTGCCGTAGAAGTTTTAAAAGCTAC
GGGTGTCGAATTTGAATTTAATCATGCCTTGGCAGGGGACGAGGTATTTGAAAAAACAGGTGTAGCACTTCCAGAAGAAA
CAATTGCTGCTGCAAAAGCTGCTGATGCAATATTATTCGGTGCTGCTGGAGAGACTGCCGCTGACGTTATTGTAAAATTG
AGAAAAATATTGGATACTTATGCAAATATAAGGCCAGTAAAATCATACAATGGAATAAACTGCCTAAAAACAGGTATCGA
CTACATAATAGTAAGGGAAAACACAGAAGGCCTCTATAAAGGTATTGAAGCTGAAATTGCAGACGGAGTTACTGTTGCAA
CAAGGGTTATTACCAAAGATGCATCTGAAAGAATATTCAAACACGCATTTGAAATGGCAAAAGACAGGAAAAAACAAGGA
ACTGCCGGAAAAGTCACATGTGCACACAAAGCAAACGTTTTAAAAATAACTGATGGGTTATTTAAGAAAACATTCTACGA
AGTTTCAAATGATTACAAAGATATACAAACTGAAGACTTTTATGTCGATGCAATGAACATGTTTATAATTTCAAGACCTG
AAACATTTGACATAGTTGTTACATCGAATTTATTTGGAGACATTTTATCAGACGGGGCTGTTGGAATGGTTGGCGGACTT
GGAATGGCACCATCAGGAAATGTTGGTGACAAATACGGTCTTTTTGAACCAATCCACGGTTCAGCTCCAGATATCGCTGG
AAAAGGACTTGCAAATCCAACTGCAACAATACTGACCGCAGTAATGATGTTAAGATACTTAAAAGAAAATGAAGCTGCTG
AAAAAGTCGAAAAAGCGCTTGAAGAAGTTTTAAGCCTTGGATTAACAACACCTGATTTAGGTGGTACTCTTTCAACAAGT
GAAATGGGTCAGGAAGTTGCAAAAAGAGTATAA

Upstream 100 bases:

>100_bases
CCAAAGGTGGTACCGGCGTTAGATTTTAATATATCGATAATCAATATAATTCCAAAAGTCAACGTAAAAACTGGGTTTGG
CCCTTAAAGGTGATTTTACA

Downstream 100 bases:

>100_bases
CTTAAAAATAACACATTAATTTTCGATTTTTTTATTTTTACTATCTAATTTATAACAATAATTGTTATTATATTATAATT
TGAAGATCTGGTGAAATCAT

Product: multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase

Products: NA

Alternate protein names: 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MHKICVIEGDGIGKEVVPAAVEVLKATGVEFEFNHALAGDEVFEKTGVALPEETIAAAKAADAILFGAAGETAADVIVKL
RKILDTYANIRPVKSYNGINCLKTGIDYIIVRENTEGLYKGIEAEIADGVTVATRVITKDASERIFKHAFEMAKDRKKQG
TAGKVTCAHKANVLKITDGLFKKTFYEVSNDYKDIQTEDFYVDAMNMFIISRPETFDIVVTSNLFGDILSDGAVGMVGGL
GMAPSGNVGDKYGLFEPIHGSAPDIAGKGLANPTATILTAVMMLRYLKENEAAEKVEKALEEVLSLGLTTPDLGGTLSTS
EMGQEVAKRV

Sequences:

>Translated_330_residues
MHKICVIEGDGIGKEVVPAAVEVLKATGVEFEFNHALAGDEVFEKTGVALPEETIAAAKAADAILFGAAGETAADVIVKL
RKILDTYANIRPVKSYNGINCLKTGIDYIIVRENTEGLYKGIEAEIADGVTVATRVITKDASERIFKHAFEMAKDRKKQG
TAGKVTCAHKANVLKITDGLFKKTFYEVSNDYKDIQTEDFYVDAMNMFIISRPETFDIVVTSNLFGDILSDGAVGMVGGL
GMAPSGNVGDKYGLFEPIHGSAPDIAGKGLANPTATILTAVMMLRYLKENEAAEKVEKALEEVLSLGLTTPDLGGTLSTS
EMGQEVAKRV
>Mature_330_residues
MHKICVIEGDGIGKEVVPAAVEVLKATGVEFEFNHALAGDEVFEKTGVALPEETIAAAKAADAILFGAAGETAADVIVKL
RKILDTYANIRPVKSYNGINCLKTGIDYIIVRENTEGLYKGIEAEIADGVTVATRVITKDASERIFKHAFEMAKDRKKQG
TAGKVTCAHKANVLKITDGLFKKTFYEVSNDYKDIQTEDFYVDAMNMFIISRPETFDIVVTSNLFGDILSDGAVGMVGGL
GMAPSGNVGDKYGLFEPIHGSAPDIAGKGLANPTATILTAVMMLRYLKENEAAEKVEKALEEVLSLGLTTPDLGGTLSTS
EMGQEVAKRV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmal

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=37.5739644970414, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4758582, Length=335, Percent_Identity=36.1194029850746, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI28178838, Length=304, Percent_Identity=36.1842105263158, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=33.5329341317365, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=33.2335329341317, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI28178819, Length=205, Percent_Identity=34.1463414634146, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.6741573033708, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=36.01108033241, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787381, Length=372, Percent_Identity=31.989247311828, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71986051, Length=336, Percent_Identity=37.5, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17505779, Length=331, Percent_Identity=37.1601208459215, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17550882, Length=335, Percent_Identity=34.0298507462687, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=35.8823529411765, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=43.3908045977011, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324709, Length=338, Percent_Identity=34.3195266272189, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324291, Length=334, Percent_Identity=34.1317365269461, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=36.4902506963788, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=39.7058823529412, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=39.7058823529412, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24661184, Length=329, Percent_Identity=36.4741641337386, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI161078637, Length=334, Percent_Identity=30.2395209580838, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI161078635, Length=334, Percent_Identity=30.2395209580838, Blast_Score=178, Evalue=6e-45,
Organism=Drosophila melanogaster, GI161078633, Length=334, Percent_Identity=30.2395209580838, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24650122, Length=334, Percent_Identity=30.2395209580838, Blast_Score=177, Evalue=6e-45,
Organism=Drosophila melanogaster, GI161078639, Length=330, Percent_Identity=30.3030303030303, Blast_Score=174, Evalue=6e-44,
Organism=Drosophila melanogaster, GI281362242, Length=330, Percent_Identity=34.2424242424242, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24648872, Length=330, Percent_Identity=34.2424242424242, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI20130355, Length=327, Percent_Identity=29.3577981651376, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550436, Length=302, Percent_Identity=22.1854304635762, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85; =1.1.1.83 [H]

Molecular weight: Translated: 35318; Mature: 35318

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKICVIEGDGIGKEVVPAAVEVLKATGVEFEFNHALAGDEVFEKTGVALPEETIAAAKA
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHH
ADAILFGAAGETAADVIVKLRKILDTYANIRPVKSYNGINCLKTGIDYIIVRENTEGLYK
HCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHH
GIEAEIADGVTVATRVITKDASERIFKHAFEMAKDRKKQGTAGKVTCAHKANVLKITDGL
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHH
FKKTFYEVSNDYKDIQTEDFYVDAMNMFIISRPETFDIVVTSNLFGDILSDGAVGMVGGL
HHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCEEHHCCC
GMAPSGNVGDKYGLFEPIHGSAPDIAGKGLANPTATILTAVMMLRYLKENEAAEKVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EEVLSLGLTTPDLGGTLSTSEMGQEVAKRV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHKICVIEGDGIGKEVVPAAVEVLKATGVEFEFNHALAGDEVFEKTGVALPEETIAAAKA
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHH
ADAILFGAAGETAADVIVKLRKILDTYANIRPVKSYNGINCLKTGIDYIIVRENTEGLYK
HCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCHHHHH
GIEAEIADGVTVATRVITKDASERIFKHAFEMAKDRKKQGTAGKVTCAHKANVLKITDGL
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEHHHH
FKKTFYEVSNDYKDIQTEDFYVDAMNMFIISRPETFDIVVTSNLFGDILSDGAVGMVGGL
HHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCEEHHCCC
GMAPSGNVGDKYGLFEPIHGSAPDIAGKGLANPTATILTAVMMLRYLKENEAAEKVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
EEVLSLGLTTPDLGGTLSTSEMGQEVAKRV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]